Human FCRL4/FcRH4 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human FCRL4/FcRH4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant human (rh) IRTA2, rhIRTA3, rhIRTA4, and rhIRTA5 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human FCRL4/FcRH4
    Gln16-Asp385
    Accession # Q96PJ5
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human small intestine (Peyer's patch) and tonsil
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human FCRL4/FcRH4 by Western Blot. Western blot shows lysates of RPMI 8226 human multiple myeloma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FCRL4/FcRH4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2426) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FCRL4/FcRH4 at approximately 58 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FCRL4/FcRH4
FcRH4, also known as IRTA1 (immunoglobulin superfamily receptor translocation associated 1), is a member of the Ig superfamily. It shares sequence homology with the classical Fc receptors. FcRH4 is preferentially expressed in B cells and contains three potential ITIM motifs in its extracellular domain. The gene for FcRH4 is localized to the human chromosome Iq 21‑23 region, a hotspot for translocation events.
  • Long Name:
    Fc Receptor-like protein 4
  • Entrez Gene IDs:
    83417 (Human)
  • Alternate Names:
    CD307d antigen; CD307d; Fc receptor homolog 4; Fc receptor-like 4; FcRH4; FCRH4Fc receptor-like protein 4; FCRL4; FcR-like protein 4; hIFGP2; IFGP family protein 2; IFGP2; IGFP2; Immune receptor translocation-associated protein 1; immunoglobulin superfamily Fc receptor, gp42; immunoglobulin superfamily receptor translocation associated 1; IRTA1; IRTA1MGC150523; MGC150522
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