Human FoxP1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human FoxP1 in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 542721
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human FoxP1
    Leu231-Gly420
    Accession # Q9H334
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human FoxP1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB4534) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific bands for FoxP1 were detected at approximately 60, 85, and 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
FoxP1 in MCF‑7 Human Cell Line. FoxP1 was detected in immersion fixed MCF-7 human breast cancer cell line using Mouse Anti-Human FoxP1 Monoclonal Antibody (Catalog # MAB4534) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FoxP1
Forkhead Box P1 (FoxP1) is a member of the FOX family of transcription factors. Alternative splicing of human FoxP1 results in multiple transcript variants encoding different isoforms. FoxP1 contains both a DNA binding domain as well as protein‑protein interaction domains. FoxP1 can homo or heterodimerize with FoxP2 and FoxP4, with dimerization necessary for DNA binding and transcriptional activity. FoxP1 has been implicated in cardiac, lung, and lymphocyte development. FoxP1 knock out mice die at embryonic day 14.5 due to heart valve and outflow tract abnormalities. FoxP1 shows both oncogenic and tumor suppressive characteristics. Human isoforms of 489 to 677 amino acids contain alternate sequences within the first 60 amino acids and/or deletion of amino acids.
  • Long Name:
    Forkhead Box P1
  • Entrez Gene IDs:
    27086 (Human); 108655 (Mouse); 297480 (Rat)
  • Alternate Names:
    FLJ23741; fork head-related protein like B; forkhead box P1; forkhead box protein P1; FoxP1; glutamine-rich factor 1; hFKH1B; HSPC215; MGC12942; MGC88572; MGC99551; QRF1; QRF112CC4
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