Intracellular Staining by Flow Cytometry
|Detection of Galanin in U-87MG Human Cell Line by Flow Cytometry. U‑87 MG human glioblastoma/astrocytoma cell line was stained with Human Galanin Monoclonal Antibody (Catalog # MAB5854, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by PerCP-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0114). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.|
|Galanin in Human Placenta. Galanin was detected in immersion fixed paraffin-embedded sections of human placenta using Human Galanin Monoclonal Antibody (Catalog # MAB5854) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
Have you used Human Galanin Antibody?
Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.
Read what people are saying who have used Human Galanin Antibody.
We have 1 review tested in 1 species: Human.We have 1 review tested in 1 application: Immunohistochemistry.
Immunohistochemistry: Human Galanin Antibody [MAB5854]
Other Experimental Details
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided