Detection of Galanin in U-87MG Human Cell Line by Flow Cytometry.
U‑87 MG human glioblastoma/astrocytoma cell line was stained with Human Galanin Monoclonal Antibody (Catalog # MAB5854, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by PerCP-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0114). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Galanin in Human Placenta.
Galanin was detected in immersion fixed paraffin-embedded sections of human placenta using Human Galanin Monoclonal Antibody (Catalog # MAB5854) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Galanin (Glycine-Alanine, representing the N-and C-terminal amino acids in bovine Galanin; also GAL) is a 3 kDa (predicted), secreted member of the Galanin family of peptides. It is co-expressed with differing neuropeptides in a variety of neuron cell types. Galanin affects multiple metabolic processes by binding to one of three GPCRs. GalR1 blocks insulin secretion, GalR2 initiates neurogenesis and GalR3 influences addictive behavior. The human Galanin proform is 11‑12 kDa in size (predicted) and 104 amino acids (aa) in length. Proteolytic processing generates a 30 aa mature Galanin peptide (aa 33‑62), plus a phosphorylated (Ser117), C-terminal 59 aa GMAP fragment that is apparently involved in the processing of noxious stimuli. Once secreted, Galanin can undergo additional proteolytic degradation. Over aa 20‑123, the human Galanin proform shares 72% aa identity with mouse Galanin proform.
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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunohistochemistry.