Human Galanin Antibody

(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Galanin in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 581403
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E.coli-derived recombinant human Galanin
    Ala20-Ser123
    Accession # P22466
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-reported
    This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Galanin in U-87MG Human Cell Line by Flow Cytometry. U‑87 MG human glioblastoma/astrocytoma cell line was stained with Human Galanin Monoclonal Antibody (Catalog # MAB5854, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by PerCP-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0114). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Immunohistochemistry
Galanin in Human Placenta. Galanin was detected in immersion fixed paraffin-embedded sections of human placenta using Human Galanin Monoclonal Antibody (Catalog # MAB5854) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblast cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galanin
Galanin (Glycine-Alanine, representing the N-and C-terminal amino acids in bovine Galanin; also GAL) is a 3 kDa (predicted), secreted member of the Galanin family of peptides. It is co-expressed with differing neuropeptides in a variety of neuron cell types. Galanin affects multiple metabolic processes by binding to one of three GPCRs. GalR1 blocks insulin secretion, GalR2 initiates neurogenesis and GalR3 influences addictive behavior. The human Galanin proform is 11‑12 kDa in size (predicted) and 104 amino acids (aa) in length. Proteolytic processing generates a 30 aa mature Galanin peptide (aa 33‑62), plus a phosphorylated (Ser117), C-terminal 59 aa GMAP fragment that is apparently involved in the processing of noxious stimuli. Once secreted, Galanin can undergo additional proteolytic degradation. Over aa 20‑123, the human Galanin proform shares 72% aa identity with mouse Galanin proform.
  • Entrez Gene IDs:
    51083 (Human); 14419 (Mouse); 29141 (Rat)
  • Alternate Names:
    GAL; GAL1; galanin prepropeptide; Galanin; GALN; GALNgalanin-related peptide; GLNNgalanin-message-associated peptide; GMAP; MGC40167
Related Research Areas
Cell and Tissue Staining Kits
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Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

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Immunohistochemistry Reagents
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Mounting Medium

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Antigen Retrieval Reagent Sampler (50 mL each)

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Antigen Retrieval Reagent-Acidic

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VisUCyte Antigen Retrieval Reagent-Universal

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VisUCyte Antigen Retrieval Reagent-Basic

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Isotype Controls
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Mouse IgG2B Isotype Control

Ctrl MAB004 32  
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Mouse IgG2B Isotype Control

Ctrl MAB0041 7  
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Mouse IgG2B Isotype Control

Ctrl MAB0042 6
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2B APC-conjugated Antibody

Flow F0133 1  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Mouse IgG2B Antibody

Flow MAB0043  
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Rat Anti-Mouse IgG2B PE-conjugated Antibody

Flow F0132  
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Rat Anti-Mouse IgG2B Fluorescein-conjugated Antibody

Flow F0134 1
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Average Rating: 4 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Human Galanin Antibody MAB5854
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Very Good
 IHC Human Anonymous 08/08/2016
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Immunohistochemistry Human Galanin Antibody MAB5854
Immunohistochemistry: Human Galanin Antibody [MAB5854]

Summary

ApplicationImmunohistochemistry
Sample TestedAdult brain
SpeciesHuman

Other Experimental Details

Other Experimental DetailsIn conjunction with SS kit (1:7000 dilution) for IHC and 1:200 for IF. FFPE human samples.

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