Galanin R2 in Human Brain. Galanin R2 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Human Galanin R2 Monoclonal Antibody (Catalog # MAB6544) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle cells of blood vessels. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galanin R2/GALR2
GALR2 (Galanin Receptor 2) is one of three 54-60 kDa G-protein coupled multipass transmembrane receptors for the Galanin neuropeptide. Galanin is co-expressed with and modulates noradrenaline and serotonin systems. GALR2 is thought to modulate growth and apoptosis. Unlike GALR and GALR3, it is thought to be anti-depressive. GALR2 mRNA is found mainly in the central nervous system (except for cerebral cortex), but also in intestine, heart, kidney, liver and many cancers. The combined extracellular domains of human GALR2 share 82% and 86% amino acid identity with mouse and rat GALR2, respectively.
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