Human GDF-15 Antibody Summary
Accession # Q99988
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human GDF‑15 by Western Blot. Western blot shows lysate of HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human GDF‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF957) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GDF‑15 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human GDF‑15 by Simple WesternTM.
Simple Western lane view shows lysate of HT1080 human fibrosarcoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GDF‑15 at approximately 47 kDa (as indicated) using 5 µg/mL of Goat Anti-Human GDF‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF957) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), placental transforming growth factor-beta, prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-beta ) superfamily. GDF-15 is highly expressed in placenta and is expressed at lower levels in kidney, pancreas, prostate and colon. It is also widely expressed in brain. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of GDF-15 contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Furthermore, the carboxy-terminal domain contains two additional cysteine residues that form a fourth intrachain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the carboxy-terminal 112 amino acid residues. Mature human GDF-15 shares 66.1% and 68.7% amino acid sequence similarity with rat and mouse GDF-15, respectively, which are remarkably low homologies between species in TGF-beta superfamily. GDF-15 has been shown to have various functions, including inhibition of production of tumor necrosis factor alpha (TNF-alpha ) from lipopolysaccharide-stimulated macrophages, induction of cartilage formation, early-stage endochonadal bone formation, and promotion of neuronal survival.
- Bootcov, M.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11514.
- Böttner, M. et al. (1999) Gene 237:105.
- Fairlie, W.D. et al. (1998) J. Leukoc. Biol 65:2.
- Fairlie, W.D. et al. (2001) J B.C 20:16911.
- Bauskin, A.R. et al. (2000) EMBO J. 19:2212.
- Strelau, J. et al. (2000) J. Neurosci. 20:8597.
- Schober, A. et al. (2001) J. Comp. Neurol. 439:32.
Citations for Human GDF-15 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
Filter your results:
An autocrine/paracrine circuit of growth differentiation factor (GDF) 15 has a role for maintenance of breast cancer stem-like cells
Authors: A Sasahara, K Tominaga, T Nishimura, M Yano, E Kiyokawa, M Noguchi, M Noguchi, H Kanauchi, T Ogawa, H Minato, K Tada, Y Seto, A Tojo, N Gotoh
Sample Types: Whole Cells
Panel of three novel serum markers predicts liver stiffness and fibrosis stages in patients with chronic liver disease
Authors: M Krawczyk, S Zimmermann, G Hess, R Holz, M Dauer, J Raedle, F Lammert, F Grünhage
PLoS ONE, 2017;12(3):e0173506.
Sample Types: Serum
Applications: ELISA Development
Growth Differentiation Factor-15-Induced Contractile Activity and Extracellular Matrix Production in Human Trabecular Meshwork Cells
Invest. Ophthalmol. Vis. Sci, 2016;57(15):6482-6495.
Sample Types: Whole Tissue
Identification of macrophage inhibitory cytokine-1 in adipose tissue and its secretion as an adipokine by human adipocytes.
Authors: Ding Q, Mracek T, Gonzalez-Muniesa P, Kos K, Wilding J, Trayhurn P, Bing C
Sample Types: Cell Lysates
Applications: Western Blot
Prognostic value of growth-differentiation factor-15 in patients with non-ST-elevation acute coronary syndrome.
Authors: Wollert KC, Kempf T, Peter T, Olofsson S, James S, Johnston N, Lindahl B, Horn-Wichmann R, Brabant G, Simoons ML, Armstrong PW, Califf RM, Drexler H, Wallentin L
Sample Types: Serum
Applications: ELISA Development
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human GDF-15 Antibody
Average Rating: 5 (Based on 1 Review)
Have you used Human GDF-15 Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image