Detects human GDF-15 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 65% cross-reactivity with recombinant mouse (rm) GDF-15 is observed, and less than 1% cross-reactivity with rmGDF‑1, rmGDF-3, and rmGDF-11 is observed.
Polyclonal Goat IgG
Chinese hamster ovary cell line CHO-derived recombinant human GDF-15 Ala197-Ile308 Accession # Q99988
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human GDF‑15 by Western Blot. Western blot shows lysate of HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human GDF‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF957) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GDF‑15 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human GDF‑15 by Simple WesternTM. Simple Western lane view shows lysate of HT1080 human fibrosarcoma cell line, loaded at 0.2 mg/mL. A specific band was detected for GDF‑15 at approximately 47 kDa (as indicated) using 5 µg/mL of Goat Anti-Human GDF‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF957) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), placental transforming growth factor-beta, prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-beta ) superfamily. GDF-15 is highly expressed in placenta and is expressed at lower levels in kidney, pancreas, prostate and colon. It is also widely expressed in brain. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of GDF-15 contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Furthermore, the carboxy-terminal domain contains two additional cysteine residues that form a fourth intrachain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the carboxy-terminal 112 amino acid residues. Mature human GDF-15 shares 66.1% and 68.7% amino acid sequence similarity with rat and mouse GDF-15, respectively, which are remarkably low homologies between species in TGF-beta superfamily. GDF-15 has been shown to have various functions, including inhibition of production of tumor necrosis factor alpha (TNF-alpha ) from lipopolysaccharide-stimulated macrophages, induction of cartilage formation, early-stage endochonadal bone formation, and promotion of neuronal survival.
Bootcov, M.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11514.
Böttner, M. et al. (1999) Gene 237:105.
Fairlie, W.D. et al. (1998) J. Leukoc. Biol 65:2.
Fairlie, W.D. et al. (2001) J B.C 20:16911.
Bauskin, A.R. et al. (2000) EMBO J. 19:2212.
Strelau, J. et al. (2000) J. Neurosci. 20:8597.
Schober, A. et al. (2001) J. Comp. Neurol. 439:32.
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