Key Product Details

Species Reactivity

Validated:

Human, Primate

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Western Blot, Neutralization, ELISA Capture, ELISA Development, ELISA Development (Capture), In vivo assay, Immunoassay Development, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 147627
View all GDF-15 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human GDF-15
Ala197-Ile308
Accession # Q99988

Specificity

Detects human GDF-15 in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human GDF‑11, recombinant mouse (rm) GDF‑5, rmGDF‑6, rmGDF‑7, or rmGDF‑8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Primate GDF‑15 Antibody

Detection of Human GDF-15 by Western Blot

Detection of Human GDF-15 by Western Blot

AML cell lines highly express GDF15. a RT-qPCR analysis of different cytokines associated with the regulation of adipogenesis in AML cell lines (K562, THP-1 and HL-60). GAPDH was used as a housekeeping gene. *P < 0.05, ***P < 0.001. b and c, RT-qPCR (b) and Western blotting (c) analysis of GDF15 in different cell lines (Kasumi, HL-60, THP-1, K562 and HEL). The densitometry values of protein expression changes were indicated. beta -actin was used as an internal control for RT-qPCR and Western blotting analysis. d ELISA detection of GDF15 expression in the supernatant of THP-1 cells with different cell densities Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29566722), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human GDF-15 by Immunohistochemistry

Detection of Human GDF-15 by Immunohistochemistry

The relationship of GDF15 expression and small marrow adipocytes in AML patients. a RT-qPCR analysis of GDF15 mRNA expression in BM from AML patients (n = 15) and the controls (n = 12). The results shown are from three independent experiments. *P < 0.05. b Western blotting analysis of GDF15 protein levels in BM from AML patients and the controls. The densitometry values of protein expression changes were indicated. beta -actin protein was used as an internal control for Western blotting analysis. c and d Representative confocal images showed the expression of GDF15 and leukemic cell markers CD34 (c) or CD117 (d) in BM sections of AML patients. DAPI was used to stain the nuclei. White triangles showed the leukemic cells with GDF15+. White arrows showed the non-leukemic cells with GDF15. Scale bar represents 40 μm. e and f Scatter plot showed the positive correlation of small adipocyte volume (e) or small adipocyte number (f) with the level of GDF15 in BM of AML (n = 20, R = 0.6679, P = 0.0013, or n = 20, R = 0.7205, P = 0.003, Spearman correlation test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29566722), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Primate GDF‑15 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human GDF‑15 (Catalog # 957-GD)
under non-reducing conditions only

Human/Primate GDF-15 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human/Primate GDF‑15 Biotinylated Antibody (Catalog # BAF940)
  • Standard: Recombinant Human GDF-15 (CHO-expressed) Protein (Catalog # 957-GD)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 7 reviews rated 4.6 using MAB957 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDF-15

Growth Differentiation Factor 15 (GDF-15), also called Macrophage inhibitory cytokine-1 (MIC-1), placental transforming growth factor-beta, prostate-derived factor, and placental bone morphogenetic protein, is a divergent member of the transforming growth factor beta (TGF-beta ) superfamily. GDF-15 is highly expressed in placenta and is expressed at lower levels in kidney, pancreas, prostate and colon. It is also widely expressed in brain. Similarly to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of GDF-15 contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine knot and the single interchain disulfide bond. Furthermore, the carboxy-terminal domain contains two additional cysteine residues that form a fourth intrachain disulfide bond. Biologically active GDF-15 is a disulfide-linked homodimer of the carboxy-terminal 112 amino acid residues. Mature human GDF-15 shares 66.1% and 68.7% amino acid sequence similarity with rat and mouse GDF-15, respectively, which are remarkably low homologies between species in TGF-beta superfamily. GDF-15 has been shown to have various functions, including inhibition of production of tumor necrosis factor alpha (TNF-alpha ) from lipopolysaccharide-stimulated macrophages, induction of cartilage formation, early-stage endochonadal bone formation, and promotion of neuronal survival.

References

  1. Bootcov, M.R. et al. (1997) Proc. Natl. Acad. Sci. USA 94:11514.
  2. Böttner, M. et al. (1999) Gene 237:105.
  3. Fairlie, W.D. et al. (1998) J. Leukoc. Biol 65:2.
  4. Fairlie, W.D. et al. (2001) J B.C 20:16911.
  5. Bauskin, A.R. et al. (2000) EMBO J. 19:2212.
  6. Strelau, J. et al. (2000) J. Neurosci. 20:8597.
  7. Schober, A. et al. (2001) J. Comp. Neurol. 439:32.

Long Name

Growth Differentiation Factor 15

Alternate Names

GDF15, MIC-1, NAG-1, PDF, PLAB, PTGF-beta

Entrez Gene IDs

9518 (Human); 23886 (Mouse); 29455 (Rat)

Gene Symbol

GDF15

UniProt

Q99988

Additional GDF-15 Products

Product Documents for Human/Primate GDF‑15 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Primate GDF‑15 Antibody

For research use only

Citations for Human/Primate GDF‑15 Antibody

Customer Reviews for Human/Primate GDF‑15 Antibody (7)

4.6 out of 5
7 Customer Ratings
5 Stars
57%
4 Stars
43%
3 Stars
0%
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1 Stars
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Showing  1 - 5 of 7 reviews Showing All
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  • Human/Primate GDF-15 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/26/2021
    Human/Primate GDF‑15 Antibody MAB957
  • Human/Primate GDF-15 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Serum
    Species: Human
    Verified Customer | Posted 08/31/2021
    Human/Primate GDF‑15 Antibody MAB957
  • Human/Primate GDF-15 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Human
    Verified Customer | Posted 07/08/2020
    Human/Primate GDF‑15 Antibody MAB957
  • Human/Primate GDF-15 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/08/2019
    This antibody was used as capture in an ELISA with BAF940 as the detection. Human Serum and Plasma samples were measurable.
    Human/Primate GDF‑15 Antibody MAB957
  • Human/Primate GDF-15 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 06/11/2019
    Together with cat# BAF940 this antibody was used in an immunoassay to measure GDF-15 in human serum samples.
    Human/Primate GDF‑15 Antibody MAB957
  • Human/Primate GDF-15 Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/20/2017
  • Human/Primate GDF-15 Antibody
    Name: Ruhul Choudhury
    Application: Block/Neutralize
    Sample Tested: Extravillous trophoblasts conditioned medium
    Species: Mouse
    Verified Customer | Posted 01/22/2017
    Human uterine endothelial cells were cultured in Extravillous trophoblasts conditioned medium. Antibody was used to neutralize Extravillous trophoblast secreted GDF-15.

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