Human GHRHR Antibody Summary
Accession # Q02643
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
GHRHR in Human Pituitary. GHRHR was detected in immersion fixed paraffin-embedded sections of human pituitary using Mouse Anti-Human GHRHR Monoclonal Antibody (Catalog # MAB10735) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
GHRHR is a GPCR that binds growth hormone-releasing hormone that is expressed in the pituitary. Significant amounts of GHRHR are also found in the hypothalamus, kidney, and placenta. GHRH regulates the secretion of growth hormone that controls the metabolism and growth of every tissue through its binding with GHRHR. Malfunction in GHRHR signaling is associated with abnormal growth. GHRHR is a therapeutic target against dwarfism, gigantism, lipodystrophy, and certain cancers.
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