Human Glypican 3 Antibody Summary
Accession # P51654.1
This antibody functions as an ELISA capture antibody when paired with Sheep Anti-Human Glypican 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2119).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Glypican 3 DuoSet ELISA Kit (Catalog # DY2119) for convenient development of a sandwich ELISA or the Human Glypican 3 Quantikine ELISA Kit (Catalog # DGLY30) for a complete optimized ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human Glypican 3 ELISA Standard Curve. Recombinant Human Glypican 3 protein was serially diluted 2-fold and captured by Mouse Anti-Human Glypican 3 Monoclonal Antibody (Catalog # MAB21191) coated on a Clear Polystyrene Microplate (DY990). Sheep Anti-Human Glypican 3 Antigen Affinity-purified Polyclonal Antibody (AF2119) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Glypican 3
Glypicans (GPC) are a family of heparan sulfate proteoglycans that are attached to the cell surface by a glycosylphosphatidylinositol (GPI) anchor. Six members of this family have been identified in mammals (GPC1-GPC6). All glypican core proteins contain an N-terminal signal peptide, a large globular cysteine-rich domain (CRD) with 14 invariant cysteine residues, a stalk-like region containing the heparan sulfate attachment sites, and a C-terminal GPI attachment site. While glypican proteins do not share strong amino acid sequence identity (they range from 17-63%), the conserved cysteine residues in their CRDs suggests similarity in their three‑dimensional structure (1, 2).
Mutations in GPC3 cause a rare disorder in humans, Simpson-Golabi-Behmel Syndrome, which is characterized by pre and postnatal overgrowth of multiple tissues and organs and an increased risk for developing embryonic tumors (3). These features are also present in the mouse knock-out of GPC3 indicating that GPC3 regulates cell survival and inhibits cell proliferation during development (4). Glypican 3 has been implicated in regulating many different signaling pathways including: IGF, FGF, BMP, and Wnt. An endoproteolytic processing of GPC3 by proprotein convertases is required for the modulation of Wnt signaling (5). Direct interaction with FGF-basic has been observed and is mediated by the heparan sulfate chains (6).
- Filmus, J. and S.B. Selleck (2001) J. Clinical Invest. 108:497.
- De Cat, B and G. David (2001) Seminars in Cell & Dev. Biol. 12:117.
- Pilia, G. et al. (1996) Nat. Genet. 12: 241.
- Cano-Gauci, D.F. et al. (1999) J. Cell Biol. 146: 255.
- De Cat, B. et al. (2003) J. Cell Biol. 163:625.
- Song, H.H. et al. (1997) J. Biol. Chem. 272:7574.
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