Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1055814
View all GPR116 Primary Antibodies »

Product Specifications

Immunogen

E. coli-expressed recombinant Human GPR116 extracellular domain.
His643-Asn945

Specificity

Detects Human GPR116 in Direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GPR116 Antibody

Detection of GPR116 in Human Lung.

GPR116 was detected in immersion fixed paraffin-embedded sections of Human Lung using Mouse Anti-Human GPR116 Monoclonal Antibody (Catalog # MAB11248) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human GPR116 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Lung.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPR116

GPR116 belongs to the LN-TM7 subfamily of the G protein-coupled receptor 2 family, also known as adhesion GPCRs. It exists as a highly glycosylated disulfide-linked dimer at the cell surface. GPR116 may have a role in the regulation of acid-base balance and is also being investigated for it's involvement in adipocyte biology.

Long Name

G Protein-coupled Receptor 116

Alternate Names

FLJ90640, G protein-coupled receptor 116, Ig-Hepta homolog, KIAA0758DKFZp564O1923, KPG_001, probable G-protein coupled receptor 116

Entrez Gene IDs

221395 (Human); 224792 (Mouse); 245977 (Rat)

Gene Symbol

ADGRF5

Additional GPR116 Products

Product Documents for Human GPR116 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GPR116 Antibody

For research use only

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Protocols

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