GPR34 in A172 Human Cell Line. GPR34 was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human GPR34 Monoclonal Antibody (Catalog # MAB4617) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of GPR34 in A172 Human Cell Line by Flow Cytometry. A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR34 Monoclonal Antibody (Catalog # MAB4617, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
GPR34 is a widely expressed 75-90 kDa seven transmembrane segment glycoprotein. It is upregulated on microglia during inflammation and functions as a receptor for lysophosphatidyl-L-serine on mast cells to promote degranulation. Multiple isoforms of GPR34 may result from multiple translation initiation sites and alternative splicing. Human GPR34 shares 89% amino acid sequence identity with mouse and rat GPR34.
G Protein-coupled Receptor 34
Entrez Gene IDs:
2857 (Human); 23890 (Mouse); 554353 (Rat)
G protein-coupled receptor 34; GPR34; probable G-protein coupled receptor 34
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