Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 419859
View all GPR34 Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human GPR34
Accession # Q9UPC5

Specificity

Stains human GPR34 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human GPR34 Antibody

GPR34 antibody in A172 Human Cell Line by Immunocytochemistry (ICC).

GPR34 in A172 Human Cell Line.

GPR34 was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human GPR34 Monoclonal Antibody (Catalog # MAB4617) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of GPR34 antibody in A172 Human Cell Line antibody by Flow Cytometry.

Detection of GPR34 in A172 Human Cell Line by Flow Cytometry.

A172 human glioblastoma cell line was stained with Mouse Anti-Human GPR34 Monoclonal Antibody (Catalog # MAB4617, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human GPR34 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GPR34

GPR34 is a widely expressed 75-90 kDa seven transmembrane segment glycoprotein. It is upregulated on microglia during inflammation and functions as a receptor for lysophosphatidyl-L-serine on mast cells to promote degranulation. Multiple isoforms of GPR34 may result from multiple translation initiation sites and alternative splicing. Human GPR34 shares 89% amino acid sequence identity with mouse and rat GPR34.

Long Name

G Protein-coupled Receptor 34

Alternate Names

G protein-coupled receptor 34, probable G-protein coupled receptor 34

Entrez Gene IDs

2857 (Human); 23890 (Mouse); 554353 (Rat)

Gene Symbol

GPR34

UniProt

Q9UPC5

Additional GPR34 Products

Product Documents for Human GPR34 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human GPR34 Antibody

For research use only

Citations for Human GPR34 Antibody

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Protocols

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