Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1042202
View all GPR85 Primary Antibodies »

Product Specifications

Immunogen

Human GPR85 extracellular domain synthetic peptide

Specificity

Detects human GPR85 in cell based ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human GPR85 Antibody

GPR85 in U‑87 MG Human Cell Line.

GPR85 was detected in immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Mouse Anti-Human GPR85 Monoclonal Antibody (Catalog # MAB10761) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human GPR85 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: GPR85

GPR85 has been reported to be expressed in several areas of human and rat CNS Matsumoto et al., 2000), as well as in genital organs. ESTs have been isolated from B-cell/lung/testis, brain, testis, and embryo libraries, as well as many mouse tissue libraries.

Long Name

G Protein-coupled Receptor 85

Alternate Names

SREB2

Entrez Gene IDs

54329 (Human); 64450 (Mouse); 64020 (Rat)

Gene Symbol

GPR85

Additional GPR85 Products

Product Documents for Human GPR85 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human GPR85 Antibody

For research use only

Customer Reviews for Human GPR85 Antibody

There are currently no reviews for this product. Be the first to review Human GPR85 Antibody and earn rewards!

Have you used Human GPR85 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies