Human Growth Hormone Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10671-100
MAB10671-SP
Human Growth Hormone ELISA Standard Curve.
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Product Details
Citations (1)
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Human Growth Hormone Antibody Summary

Species Reactivity
Human
Specificity
Detects human Growth Hormone in direct ELISAs.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 178915R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human Growth Hormone
Phe27-Phe217
Accession # CAA23779
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Growth Hormone (GH) DuoSet ELISA Kit (Catalog # DY1067) for convenient development of a sandwich ELISA or the Human Growth Hormone Quantikine ELISA Kit (Catalog # DGH00) for a complete optimized ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Human Growth Hormone ELISA Standard Curve. Recombinant Human Growth Hormone protein was serially diluted 2-fold and captured by Mouse Anti-Human Growth Hormone Monoclonal Antibody (Catalog # MAB10671) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Growth Hormone

Growth hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones, GH-releasing hormone (GHRH) and somatostatin (SST), as well as by signals from the periphery, ghrelin (3) and leptin (4). The human GH cDNA encodes a 217 amino acid (aa) precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified (5). Human GH is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor, which is present in many cell types (1, 2). GH stimulates the liver and other tissues to produce IGF-I, which regulates growth and metabolism. GH has also been shown to have direct effects on growth that is independent of IGF-I. GH, directly or indirectly via IGF-I, can act on B cells, T cells, NK cells, macrophages, and neutrophils to exert immunomodulatory activities (6). In addition, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake, and protein synthesis (1, 2, 6).

References
  1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
  2. Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
  3. Kojima, K. et al. (1999) Nature 402:656.
  4. Tannenbaum, G. et al. (1998) Endocrinol. 139:3871.
  5. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
Entrez Gene IDs
2688 (Human)
Alternate Names
GH1; GHB5; GHIGHD1B; GHN; GH-N; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; IGHD1A; IGHD2; Somatotropin

Product Datasheets

Citation for Human Growth Hormone Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Structural and functional characterization of recombinant human growth hormone isolated from transgenic pig milk
    Authors: SY Lee, JH Han, EK Lee, YK Kim, SA Hwang, SH Lee, M Kim, GY Cho, JH Hwang, SJ Kim, JG Yoo, SK Cho, KJ Lee, WK Cho
    PLoS ONE, 2020;15(7):e0236788.
    Species: Porcine
    Sample Types: Protein
    Applications: Western Blot

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