Human/Mouse/Rat Growth Hormone Antibody

Catalog # Availability Size / Price Qty
AF1067
AF1067-SP
Human/Mouse/Rat Growth Hormone Antibody in Data
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Product Details
Citations (6)
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Reviews (3)

Human/Mouse/Rat Growth Hormone Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human Growth Hormone in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Growth Hormone
Phe27-Phe217
Accession # CAA23779
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Growth Hormone Monoclonal Antibody (Catalog # MAB10671).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Growth Hormone (GH) DuoSet ELISA Kit (Catalog # DY1067) for convenient development of a sandwich ELISA or the Human Growth Hormone Quantikine ELISA Kit (Catalog # DGH00) for a complete optimized ELISA.

 
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize Growth Hormone-induced proliferation in the Nb2‑11 rat lymphoma cell line. Gout, P.W. et al. (1980) Cancer Res. 40:2433. The Neutralization Dose (ND50) is typically 1.5-7.5 ng/mL in the presence of 0.2 ng/mL Recombinant Human Growth Hormone.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat Growth Hormone by Western Blot. View Larger

Detection of Human, Mouse, and Rat Growth Hormone by Western Blot. Western blot shows lysates of human, mouse, and rat pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Growth Hormone at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Growth Hormone in Human Pituitary. View Larger

Growth Hormone in Human Pituitary. Growth Hormone was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Cell Proliferation Induced by Growth Hormone and Neutralization by Human Growth Hormone Antibody. View Larger

Cell Proliferation Induced by Growth Hormone and Neutralization by Human Growth Hormone Antibody. Recombinant Human Growth Hormone (Catalog # 1067-GH) stimulates proliferation in the Nb2-11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Growth Hormone (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067). The ND50is typically 1.5-7.5 ng/mL.

Human Growth Hormone ELISA Standard Curve. Recombinant Human Growth Hormone protein was serially diluted 2-fold and captured by Mouse Anti-Human Growth Hormone Monoclonal Antibody (Catalog # MAB10671) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Growth Hormone

Growth Hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones - GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin (3) and leptin (4). The human GH cDNA encodes a 217 amino acid (aa) residue precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified (5).

Human GH is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor, which is present in many cell types (1, 2). GH stimulates the liver and other tissues to produce IGF-I, which regulates growth and metabolism. GH has also been shown to have direct effects on growth that is independent of IGF-I. GH, directly or indirectly via IGF-I, can act on B cells, T cells, NK cells, macrophages, and neutrophils to exert immunomodulatory activities (6). In addition, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake, and protein synthesis (1, 2, 6).

References
  1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
  2. Le Roith, D. et al. (2001) Endocrine Rev. 22:53.
  3. Kojima, K. et al. (1999) Nature, 402:656.
  4. Tannenbaum, G. et al. (1998) Endocrinol. 139:3871.
  5. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
Entrez Gene IDs
2688 (Human)
Alternate Names
GH1; GHB5; GHIGHD1B; GHN; GH-N; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; IGHD1A; IGHD2; Somatotropin

Product Datasheets

Citations for Human/Mouse/Rat Growth Hormone Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. PDK1-FoxO1 pathway in AgRP neurons of arcuate nucleus promotes bone formation via GHRH-GH-IGF1 axis
    Authors: H Sasanuma, M Nakata, K Parmila, J Nakae, T Yada
    Mol Metab, 2017;6(5):428-439.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  2. Growth hormone is permissive for neoplastic colon growth
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. STAT3 upregulation in pituitary somatotroph adenomas induces growth hormone hypersecretion.
    Authors: Zhou, Cuiqi, Jiao, Yonghui, Wang, Renzhi, Ren, Song-Gua, Wawrowsky, Kolja, Melmed, Shlomo
    J Clin Invest, 2015;125(4):1692-702.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Troubleshooting PEG-hGH detection supporting pharmacokinetic evaluation in growth hormone deficient patients.
    Authors: Myler HA, McVay S, Kratzsch J
    J Pharmacol Toxicol Methods, 2010;61(2):92-7.
    Species: Human
    Sample Types: Serum
    Applications: Electrochemiluminescent Assay
  5. Growth hormone is present in the human retina and vitreous fluid.
    Authors: Harvey S, Parker E, Macdonald I, Sanders EJ
    Neurosci. Lett., 2009;455(3):199-202.
    Species: Human
    Sample Types: Vitreous Humor
    Applications: Western Blot
  6. Shift of monocyte function toward cellular immunity during sleep.
    Authors: Lange T, Dimitrov S, Fehm HL, Westermann J, Born J
    Arch. Intern. Med., 2006;166(16):1695-700.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Reviews for Human/Mouse/Rat Growth Hormone Antibody

Average Rating: 4.7 (Based on 3 Reviews)

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Human/Mouse/Rat Growth Hormone Antibody
By Anonymous on 04/17/2018
Application: Flow Sample Tested: human antibody IgG Species: Human

Human/Mouse/Rat Growth Hormone Antibody
By Anonymous on 12/08/2017
Application: ELISA Sample Tested: igg Species: Goat

Human Growth Hormone Antibody
By Anonymous on 04/26/2016
Application: Func Sample Tested: Human recombinant antibody Species: Goat