Human/Mouse/Rat Growth Hormone Antibody

(6 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human Growth Hormone in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human Growth Hormone
    Phe27-Phe217
    Accession # CAA23779
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize Growth Hormone-induced proliferation in the Nb2‑11 rat lymphoma cell line. Gout, P.W. et al. (1980) Cancer Res. 40:2433. The Neutralization Dose (ND50) is typically 1.5-7.5 ng/mL in the presence of 0.2 ng/mL Recombinant Human Growth Hormone.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat Growth Hormone by Western Blot. Western blot shows lysates of human, mouse, and rat pituitary gland tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Growth Hormone at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
Growth Hormone in Human Pituitary. Growth Hormone was detected in immersion fixed paraffin-embedded sections of human pituitary using Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by Growth Hormone and Neutralization by Human Growth Hormone Antibody. Recombinant Human Growth Hormone (Catalog # 1067-GH) stimulates proliferation in the Nb2‑11 rat lymphoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Growth Hormone (0.2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Growth Hormone Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1067). The ND50 is typically 1.5-7.5 ng/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Growth Hormone

Growth Hormone (GH), also known as somatotropin, is a member of a family of growth factors that includes prolactin, placental lactogens, proliferins, and somatolactin (1, 2). It is synthesized primarily by somatotropes in the anterior pituitary and is stored in secretary granules. The pulsatile release of GH into circulation is regulated by the concerted actions of the hypothalamic hormones - GH-releasing hormone (GHRH) and somatostatin (SST) - as well as by signals from the periphery - ghrelin (3) and leptin (4). The human GH cDNA encodes a 217 amino acid (aa) residue precursor protein with a 26 aa putative signal peptide. By alternative splicing, at least four isoforms of GH have been identified (5).

Human GH is a pleiotropic cytokine that exerts its biological actions by binding to the transmembrane GH receptor, which is present in many cell types (1, 2). GH stimulates the liver and other tissues to produce IGF-I, which regulates growth and metabolism. GH has also been shown to have direct effects on growth that is independent of IGF-I. GH, directly or indirectly via IGF-I, can act on B cells, T cells, NK cells, macrophages, and neutrophils to exert immunomodulatory activities (6). In addition, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake, and protein synthesis (1, 2, 6).

  • References:
    1. Goffin, V. et al. (1996) Endocrine Rev. 17:385.
    2. Le Roith, D. et al. (2001) Endocrine Rev., 22:53.
    3. Kojima, K. et al. (1999) Nature, 402:656.
    4. Tannenbaum, G. et al. (1998) Endocrinol. 139:3871.
    5. Welniak, L.A. et al. (2002) J. Leukoc. Biol. 71:381.
  • Entrez Gene IDs:
    2688 (Human)
  • Alternate Names:
    GH1; GHIGHD1B; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; Somatotropin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. PDK1-FoxO1 pathway in AgRP neurons of arcuate nucleus promotes bone formation via GHRH-GH-IGF1 axis
    Authors: H Sasanuma, M Nakata, K Parmila, J Nakae, T Yada
    Mol Metab, 2017;6(5):428-439.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  2. Growth hormone is permissive for neoplastic colon growth
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. STAT3 upregulation in pituitary somatotroph adenomas induces growth hormone hypersecretion.
    Authors: Zhou, Cuiqi, Jiao, Yonghui, Wang, Renzhi, Ren, Song-Gua, Wawrowsky, Kolja, Melmed, Shlomo
    J Clin Invest, 2015;125(4):1692-702.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Troubleshooting PEG-hGH detection supporting pharmacokinetic evaluation in growth hormone deficient patients.
    Authors: Myler HA, McVay S, Kratzsch J
    J Pharmacol Toxicol Methods, 2010;61(2):92-7.
    Species: Human
    Sample Type: Serum
    Application: Electrochemiluminescent Assay
  5. Growth hormone is present in the human retina and vitreous fluid.
    Authors: Harvey S, Parker E, Macdonald I, Sanders EJ
    Neurosci. Lett., 2009;455(3):199-202.
    Species: Human
    Sample Type: Vitreous Humor
    Application: WB
  6. Shift of monocyte function toward cellular immunity during sleep.
    Authors: Lange T, Dimitrov S, Fehm HL, Westermann J, Born J
    Arch. Intern. Med., 2006;166(16):1695-700.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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Summary

ApplicationFunctional Assay
Sample TestedHuman recombinant antibody
SpeciesGoat

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