Human HGF Antibody

Catalog # Availability Size / Price Qty
MAB294-SP
MAB294-100
MAB294-500
Detection of Recombinant Human, Mouse, and Canine HGF by Western Blot.
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Product Details
Citations (19)
FAQs
Supplemental Products
Reviews (1)

Human HGF Antibody Summary

Species Reactivity
Human
Specificity
Detects human HGF in Western blots. In Western blots, no-cross reactivity with recombinant mouse HGF or recombinant canine HGF is observed.
Source
Monoclonal Mouse IgG1 Clone # 24612
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HGF
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Accession # P14210
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below
Neutralization
Measured by its ability to neutralize HGF-induced proliferation in the 4MBr‑5 rhesus monkey epithelial cell line. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 100 ng/mL Recombinant Human HGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Human, Mouse, and Canine HGF antibody by Western Blot. View Larger

Detection of Recombinant Human, Mouse, and Canine HGF by Western Blot. Western blot shows 25 ng of Recombinant Human HGF (Catalog # 294-HG), Recombinant Mouse HGF (2207-HG) and Recombinant Canine HGF (3386-HG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HGF at approximately 75 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry HGF antibody in Human Lung Adenocarcinoma Tissue by Immunohistochemistry (IHC-P). View Larger

HGF in Human Lung Adenocarcinoma Tissue. HGF was detected in immersion fixed paraffin-embedded sections of human lung adenocarcinoma tissue using Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB294) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (brown VC001) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Neutralization Cell Proliferation Induced by HGF and Neutralization by Human HGF Antibody. View Larger

Cell Proliferation Induced by HGF and Neutralization by Human HGF Antibody. Recombinant Human HGF (Catalog # 294-HG) stimulates proliferation in the 4MBr-5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HGF (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB294). The ND50 is typically 0.1-0.3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HGF

HGF, also known as Hepatopoietin A and Scatter Factor, is a liver- and fibroblast-derived soluble protein. It interacts with HGF R to promote mitogenesis and morphogenesis of endothelial and epithelial cells.

Long Name
Hepatocyte Growth Factor
Entrez Gene IDs
3082 (Human); 15234 (Mouse); 24446 (Rat); 403441 (Canine); 102133907 (Cynomolgus Monkey); 493705 (Feline)
Alternate Names
deafness, autosomal recessive 39; DFNB39; EC 3.4.21; EC 3.4.21.7; fibroblast-derived tumor cytotoxic factor; F-TCF; hepatocyte growth factor (hepapoietin A; scatter factor); Hepatopoeitin-A; Hepatopoietin A; HGF; HGFB; HPTA; HPTAhepatocyte growth factor; lung fibroblast-derived mitogen; Scatter factor; SF; SFhepatopoeitin-A

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Citations for Human HGF Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

19 Citations: Showing 1 - 10
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  1. LIFR recruits HGF-producing neutrophils to promote liver injury repair and regeneration
    Authors: Y Deng, Z Zhao, M Sheldon, Y Zhao, H Teng, C Martinez, J Zhang, C Lin, Y Sun, F Yao, H Zhu, L Ma
    bioRxiv : the preprint server for biology, 2023-03-21;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  2. Bone Marrow-Derived Vasculogenic Mesenchymal Stem Cells Enhance In Vitro Angiogenic Sprouting of Human Umbilical Vein Endothelial Cells
    Authors: HH Jang, Y Son, G Park, KS Park
    International Journal of Molecular Sciences, 2022-12-27;24(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Deciphering the Molecular Mechanism of Spontaneous Senescence in Primary Epithelial Ovarian Cancer Cells
    Authors: M Paku?a, E Ma?y, P Uruski, A Witucka, M Bogucka, N Jaroszewsk, N Makowska, A Niklas, R Moszy?ski, S Sajdak, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Cancers (Basel), 2020-01-27;12(2):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer
    Authors: M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek
    Cancers (Basel), 2019-05-13;11(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
    Authors: M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek
    Int J Mol Sci, 2019-01-02;20(1):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Targeting angiogenesis in multiple myeloma by the VEGF and HGF blocking DARPin� protein MP0250: a preclinical study
    Authors: L Rao, K De Veirman, D Giannico, I Saltarella, V Desantis, MA Frassanito, AG Solimando, D Ribatti, M Prete, A Harstrick, U Fiedler, H De Raeve, V Racanelli, K Vanderkerk, A Vacca
    Oncotarget, 2018-01-30;9(17):13366-13381.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. High EMT Signature Score of Invasive Non-Small Cell Lung Cancer (NSCLC) Cells Correlates with NFkappaB Driven Colony-Stimulating Factor 2 (CSF2/GM-CSF) Secretion by Neighboring Stromal Fibroblasts.
    Authors: Rudisch A, Dewhurst M, Horga L, Kramer N, Harrer N, Dong M, van der Kuip H, Wernitznig A, Bernthaler A, Dolznig H, Sommergruber W
    PLoS ONE, 2015-04-28;10(4):e0124283.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts.
    Authors: Angelucci C, Maulucci G, Colabianchi A, Iacopino F, D'Alessio A, Maiorana A, Palmieri V, Papi M, De Spirito M, Di Leone A, Masetti R, Sica G
    Br J Cancer, 2015-04-16;112(10):1675-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Hepatocyte growth factor and p38 promote osteogenic differentiation of human mesenchymal stem cells.
    Authors: Aenlle K, Curtis K, Roos B, Howard G
    Mol Endocrinol, 2014-03-27;28(5):722-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
    Authors: Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G
    Cell Stem Cell, 2014-03-06;14(3):342-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  11. Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesis.
    Authors: Tyan SW, Kuo WH, Huang CK
    PLoS ONE, 2011-01-13;6(1):e15313.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Neutralization, Western Blot
  12. A multiplex immunoassay for human adipokine profiling.
    Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
    Clin. Chem., 2010-06-08;56(0):1320.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Luminex Development
  13. Soluble c-Met receptors inhibit phosphorylation of c-Met and growth of hepatocyte growth factor: c-Met-dependent tumors in animal models.
    Authors: Coxon A, Rex K, Meyer S, Sun J, Sun J, Chen Q, Radinsky R, Kendall R, Burgess TL
    Mol. Cancer Ther., 2009-05-12;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Neutralization
  14. alpha vbeta 6 Integrin promotes the invasion of morphoeic basal cell carcinoma through stromal modulation.
    Authors: Marsh D, Dickinson S, Neill GW, Marshall JF, Hart IR, Thomas GJ
    Cancer Res., 2008-05-01;68(9):3295-303.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  15. Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
    Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
    Int. J. Cancer, 2006-11-15;119(10):2313-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005-11-01;175(9):6177-89.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  17. Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3.
    Authors: Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC
    Cancer Res., 2003-02-15;63(4):887-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Hepatocyte growth factor and vascular endothelial growth factor expression in pig-to-primate xenotransplanted hearts.
    Authors: Takayama K, Suzuki J, Kawauchi M, Tsukioka K, Wada Y, Zhang T, Endoh M, Takamoto S, Amano J, Isobe M
    Transplant. Proc., 2000-08-01;32(5):987-9.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-P

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Human HGF Antibody
By Anonymous on 09/08/2017
Application: WB Sample Tested: Cancer cell lysates Species: Human