Human HGF Antibody
Human HGF Antibody Summary
Gln32-Ser728 (Asp384Asn, Asp416Asn, Leu622Ser, His645Arg, Val678Ile)
Accession # P14210
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Recombinant Human, Mouse, and Canine HGF by Western Blot. Western blot shows 25 ng of Recombinant Human HGF (Catalog # 294-HG), Recombinant Mouse HGF (2207-HG) and Recombinant Canine HGF (3386-HG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB294) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for HGF at approximately 75 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 3.
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HGF in Human Lung Adenocarcinoma Tissue. HGF was detected in immersion fixed paraffin-embedded sections of human lung adenocarcinoma tissue using Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB294) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (brown VC001) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
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Cell Proliferation Induced by HGF and Neutralization by Human HGF Antibody. Recombinant Human HGF (Catalog # 294-HG) stimulates proliferation in the 4MBr-5 rhesus monkey epithelial cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human HGF (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human HGF Monoclonal Antibody (Catalog # MAB294). The ND50 is typically 0.1-0.3 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HGF
HGF, also known as Hepatopoietin A and Scatter Factor, is a liver- and fibroblast-derived soluble protein. It interacts with HGF R to promote mitogenesis and morphogenesis of endothelial and epithelial cells.
Product Datasheets
Citations for Human HGF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
16
Citations: Showing 1 - 10
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Deciphering the Molecular Mechanism of Spontaneous Senescence in Primary Epithelial Ovarian Cancer Cells
Authors: M Paku?a, E Ma?y, P Uruski, A Witucka, M Bogucka, N Jaroszewsk, N Makowska, A Niklas, R Moszy?ski, S Sajdak, A Tykarski, J Miku?a-Pie, K Ksi??ek
Cancers (Basel), 2020;12(2):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells
Authors: M Paku?a, J Miku?a-Pie, A Witucka, K Kostka-Jez, P Uruski, R Moszy?ski, E Naumowicz, S Sajdak, A Tykarski, K Ksi??ek
Int J Mol Sci, 2019;20(1):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer
Authors: M Paku?a, P Uruski, A Niklas, A Wo?niak, D Szpurek, A Tykarski, J Miku?a-Pie, K Ksi??ek
Cancers (Basel), 2019;11(5):.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Targeting angiogenesis in multiple myeloma by the VEGF and HGF blocking DARPin� protein MP0250: a preclinical study
Authors: L Rao, K De Veirman, D Giannico, I Saltarella, V Desantis, MA Frassanito, AG Solimando, D Ribatti, M Prete, A Harstrick, U Fiedler, H De Raeve, V Racanelli, K Vanderkerk, A Vacca
Oncotarget, 2018;9(17):13366-13381.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
CD44v6 is a marker of constitutive and reprogrammed cancer stem cells driving colon cancer metastasis.
Authors: Todaro M, Gaggianesi M, Catalano V, Benfante A, Iovino F, Biffoni M, Apuzzo T, Sperduti I, Volpe S, Cocorullo G, Gulotta G, Dieli F, De Maria R, Stassi G
Cell Stem Cell, 2014;14(3):342-56.
Species: Human
Sample Types: Whole Cells
Applications: Functional Assay -
Hepatocyte growth factor and p38 promote osteogenic differentiation of human mesenchymal stem cells.
Authors: Aenlle K, Curtis K, Roos B, Howard G
Mol Endocrinol, 2014;28(5):722-30.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Breast cancer cells induce cancer-associated fibroblasts to secrete hepatocyte growth factor to enhance breast tumorigenesis.
Authors: Tyan SW, Kuo WH, Huang CK
PLoS ONE, 2011;6(1):e15313.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A multiplex immunoassay for human adipokine profiling.
Authors: Schipper HS, De Jager W, van Dijk ME, Meerding J, Zelissen PM, Adan RA, Prakken BJ, Kalkhoven E
Clin. Chem., 2010;56(0):1320.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Luminex Development -
Soluble c-Met receptors inhibit phosphorylation of c-Met and growth of hepatocyte growth factor: c-Met-dependent tumors in animal models.
Authors: Coxon A, Rex K, Meyer S, Sun J, Sun J, Chen Q, Radinsky R, Kendall R, Burgess TL
Mol. Cancer Ther., 2009;0(0):.
Species: Human
Sample Types: Recombinant Protein
Applications: Neutralization -
alpha vbeta 6 Integrin promotes the invasion of morphoeic basal cell carcinoma through stromal modulation.
Authors: Marsh D, Dickinson S, Neill GW, Marshall JF, Hart IR, Thomas GJ
Cancer Res., 2008;68(9):3295-303.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Microvascular endothelial cells increase proliferation and inhibit apoptosis of native human acute myelogenous leukemia blasts.
Authors: Hatfield K, Ryningen A, Corbascio M, Bruserud O
Int. J. Cancer, 2006;119(10):2313-21.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
J. Immunol., 2005;175(9):6177-89.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization -
Expression of the CD44v2-10 isoform confers a metastatic phenotype: importance of the heparan sulfate attachment site CD44v3.
Authors: Barbour AP, Reeder JA, Walsh MD, Fawcett J, Antalis TM, Gotley DC
Cancer Res., 2003;63(4):887-92.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Hepatocyte growth factor and vascular endothelial growth factor expression in pig-to-primate xenotransplanted hearts.
Authors: Takayama K, Suzuki J, Kawauchi M, Tsukioka K, Wada Y, Zhang T, Endoh M, Takamoto S, Amano J, Isobe M
Transplant. Proc., 2000;32(5):987-9.
Species: Porcine
Sample Types: Whole Tissue
Applications: IHC-P -
High EMT Signature Score of Invasive Non-Small Cell Lung Cancer (NSCLC) Cells Correlates with NFkappaB Driven Colony-Stimulating Factor 2 (CSF2/GM-CSF) Secretion by Neighboring Stromal Fibroblasts.
Authors: Rudisch A, Dewhurst M, Horga L, Kramer N, Harrer N, Dong M, van der Kuip H, Wernitznig A, Bernthaler A, Dolznig H, Sommergruber W
PLoS ONE, 0;10(4):e0124283.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
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