Hepatocyte Nuclear Factor 4 alpha (HNF4 alpha; NR2A1) is a member of the orphan nuclear receptor family. HNF4 alpha is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9. The expression of HNF-4 gamma (NR2A2) is observed in the pancreas, kidney, small intestine, and testes with no expression detected in the liver. The level of HNF-4 gamma is less 10% than that of HNF-4 alpha.
Human HNF-4 gamma/NR2A2 Antibody (N3224)
R&D Systems | Catalog # PP-N3224-00
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # N3224
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Product Specifications
Immunogen
Human HNF-4 gamma/NR2A2
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human HNF-4 gamma/NR2A2 Antibody (N3224)
HNF‑4 gamma /NR2A2 in Rat Small Intestine.
HNF‑4 gamma /NR2A2 was detected in perfusion fixed paraffin-embedded sections of rat small intestine using HNF‑4 gamma /NR2A2 Monoclonal Antibody (Catalog # PP‑N3224‑00) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of HNF-4 gamma/NR2A2 by Western Blot
HNF4G promotes resistance to cisplatin in lung adenocarcinoma cells.(A) qRT-PCR and (B) western blot results of HNF4G expression after cisplatin treatment in A549 and HCC827 cells. (C) qRT-PCR results of HNF4G expression in HNF4G overexpression and knockdown cells. (D) Cisplatin sensitivity curves of HNF4G overexpression and knockdown cells. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36923501), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of HNF-4 gamma/NR2A2 by Western Blot
HNF4G promotes resistance to cisplatin in lung adenocarcinoma cells.(A) qRT-PCR and (B) western blot results of HNF4G expression after cisplatin treatment in A549 and HCC827 cells. (C) qRT-PCR results of HNF4G expression in HNF4G overexpression and knockdown cells. (D) Cisplatin sensitivity curves of HNF4G overexpression and knockdown cells. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36923501), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human HNF-4 gamma/NR2A2 Antibody (N3224)
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed paraffin-embedded sections of rat small intestine
Sample: Perfusion fixed paraffin-embedded sections of rat small intestine
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using PP-N3224-00 in the following applications:
Formulation, Preparation, and Storage
Formulation
A liquid formulation in physiologic saline with 0.1% NaN<sub>3</sub>.
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: HNF-4 gamma/NR2A2
Long Name
Hepatocyte Nuclear Factor 4 gamma
Alternate Names
HNF4 gamma, HNF4G, NR2A2
Entrez Gene IDs
3174 (Human)
Gene Symbol
HNF4G
Additional HNF-4 gamma/NR2A2 Products
Product Documents for Human HNF-4 gamma/NR2A2 Antibody (N3224)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human HNF-4 gamma/NR2A2 Antibody (N3224)
For research use only
Related Research Areas
Citations for Human HNF-4 gamma/NR2A2 Antibody (N3224)
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Customer Reviews for Human HNF-4 gamma/NR2A2 Antibody (N3224) (1)
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Application: ImmunohistochemistrySample Tested: Small intestine tissueSpecies: HumanVerified Customer | Posted 10/23/2022
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
