Human HNF-4 gamma/NR2A2 MAb (Clone N3224) Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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HNF‑4 gamma /NR2A2 in Rat Small Intestine. HNF‑4 gamma /NR2A2 was detected in perfusion fixed paraffin-embedded sections of rat small intestine using HNF‑4 gamma /NR2A2 Monoclonal Antibody (Catalog # PP‑N3224‑00) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Detection of HNF-4 gamma/NR2A2 by Western Blot HNF4G promotes resistance to cisplatin in lung adenocarcinoma cells.(A) qRT-PCR and (B) western blot results of HNF4G expression after cisplatin treatment in A549 and HCC827 cells. (C) qRT-PCR results of HNF4G expression in HNF4G overexpression and knockdown cells. (D) Cisplatin sensitivity curves of HNF4G overexpression and knockdown cells. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36923501), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Detection of HNF-4 gamma/NR2A2 by Western Blot HNF4G promotes resistance to cisplatin in lung adenocarcinoma cells.(A) qRT-PCR and (B) western blot results of HNF4G expression after cisplatin treatment in A549 and HCC827 cells. (C) qRT-PCR results of HNF4G expression in HNF4G overexpression and knockdown cells. (D) Cisplatin sensitivity curves of HNF4G overexpression and knockdown cells. * p < 0.05, ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36923501), licensed under a CC-BY license. Not internally tested by R&D Systems.
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Preparation and Storage
Background: HNF-4 gamma/NR2A2
Hepatocyte Nuclear Factor 4 alpha (HNF4 alpha; NR2A1) is a member of the orphan nuclear receptor family. HNF4 alpha is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9. The expression of HNF-4 gamma (NR2A2) is observed in the pancreas, kidney, small intestine, and testes with no expression detected in the liver. The level of HNF-4 gamma is less 10% than that of HNF-4 alpha.
Product Datasheets
Citation for Human HNF-4 gamma/NR2A2 MAb (Clone N3224)
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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HNF4G increases cisplatin resistance in lung adenocarcinoma via the MAPK6/Akt pathway
Authors: J Liang, G Zhao, Y Bian, G Bi, Q Sui, H Zhang, H Shi, G Shan, Y Huang, Z Chen, L Wang, C Zhan
PeerJ, 2023-03-10;11(0):e14996.
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