Human HNF-4 gamma/NR2A2 Antibody

  
  • Species Reactivity
    Human
  • Source
    Monoclonal Mouse IgG2a Clone # N3224
  • Formulation
    A liquid formulation in physiologic saline with 0.1% NaN3.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    Please refer to the PDF datasheet for specifications.
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
HNF‑4 gamma /NR2A2 in Rat Small Intestine. HNF‑4 gamma /NR2A2 was detected in perfusion fixed paraffin-embedded sections of rat small intestine using HNF‑4 gamma /NR2A2 Monoclonal Antibody (Catalog # PP‑N3224‑00) overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Stability & Storage
    Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: HNF-4 gamma/NR2A2

Hepatocyte Nuclear Factor 4 alpha (HNF4 alpha; NR2A1) is a member of the orphan nuclear receptor family. HNF4 alpha is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9. The expression of HNF-4 gamma (NR2A2) is observed in the pancreas, kidney, small intestine, and testes with no expression detected in the liver. The level of HNF-4 gamma is less 10% than that of HNF-4 alpha.

  • Long Name:
    Hepatocyte Nuclear Factor 4 gamma
  • Entrez Gene IDs:
    3174 (Human)
  • Alternate Names:
    hepatocyte nuclear factor 4, gamma; hepatocyte nuclear factor 4-gamma; HNF4 gamma; HNF-4 gamma; HNF4G; HNF-4-gamma; NR2A2; NR2A2Nuclear receptor subfamily 2 group A member 2; NR2A3
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