Human HTRA1/PRSS11 Antibody Summary
Accession # Q92743
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
HTRA1/PRSS11 in Human Hepatocellular Carcinoma and IBS Intestine. HTRA1/PRSS11 was detected in immersion fixed paraffin-embedded sections of human fibrolamellar hepatocellular carcinoma and IBS intestine (positive staining; upper panels) and human cervical squamous cell carcinoma and smooth muscle (negative staining; lower panels) using Mouse Anti-Human HTRA1/PRSS11 Monoclonal Antibody (Catalog # MAB29161) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane (luminal surface of intestinal gland). View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
HTRA1 is a serine protease encoded by the PRSS11 gene and is expressed in many tissues. HTRA1 regulates the availability of IGFs by cleaving IGFBPs.
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Cell and Tissue Staining Kits
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