Human HTRA1/PRSS11 Antibody

Catalog # Availability Size / Price Qty
MAB29161-100
MAB29161-SP
Human HTRA1/PRSS11 Antibody in Immunohistochemistry
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Human HTRA1/PRSS11 Antibody Summary

Species Reactivity
Human
Specificity
Detects human HTRA1/PRSS11 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 275603
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human HTRA1/PRSS11
Gln23-Pro480
Accession # Q92743
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry HTRA1/PRSS11  in Human Hepatocellular Carcinoma and IBS Intestine. View Larger

HTRA1/PRSS11 in Human Hepatocellular Carcinoma and IBS Intestine. HTRA1/PRSS11 was detected in immersion fixed paraffin-embedded sections of human fibrolamellar hepatocellular carcinoma and IBS intestine (positive staining; upper panels) and human cervical squamous cell carcinoma and smooth muscle (negative staining; lower panels) using Mouse Anti-Human HTRA1/PRSS11 Monoclonal Antibody (Catalog # MAB29161) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane (luminal surface of intestinal gland). View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HTRA1/PRSS11

HTRA1 is a serine protease encoded by the PRSS11 gene and is expressed in many tissues. HTRA1 regulates the availability of IGFs by cleaving IGFBPs.

Long Name
High Temperature Requirement Protein-1
Entrez Gene IDs
5654 (Human)
Alternate Names
ARMD7; EC 3.4.21; EC 3.4.21.108; High-temperature requirement A serine peptidase 1; HtrA serine peptidase 1; HTRA1; IGFBP5-protease; L56; ORF480; PRSS11; Serine protease 11; serine, 11 (IGF binding)

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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