Human IDO2 Antibody

Catalog # Availability Size / Price Qty
MAB10098-100
MAB10098-SP
IDO2 in  Human Liver.
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Human IDO2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IDO2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1001802
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing  human IDO2
Met1-Val407
Accession # Q6ZQW0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry IDO2 in  Human Liver. View Larger

IDO2 in Human Liver. IDO2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human IDO2 Monoclonal Antibody (Catalog # MAB10098) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IDO2

Indoleamine 2,3-dioxygenase (IDO2) is a 47 kDa heme-containing cytosolic dioxygenase. Human IDO2 shares 64% aa sequence identity with mouse IDO2. IDO2 is one of three dioxygenases capable of catalyzing the first and rate-limiting step of the L-kynurenine pathway (KP): oxidative cleavage of the essential amino acid L-tryptophan to form N formyl kynurenine (1). Of these proteins, IDO1 and IDO2 are both related, monomeric enzymes but share only 38% aa sequence identity. The IDO isoforms are not functionally redundant. Although expression of IDO2 has been upregulated in some cancers (2,3), IDO2 expression is generally restricted to the liver, kidney, brain, and certain immune cell types unlike the more ubiquitously expressed indoleamine 2,3-dioxygenase (IDO) (1). Differential inhibition of IDO1 and IDO2 is observed with several molecules (4,5). IDO2 has significantly lower tryptophan catabolic activity than IDO1 and TDO2 (4, 6-8) suggesting it does not play a significant physiological role in the KP. Instead, IDO2 may have an alternative functional role: either non-enzymatic or utilizing a more physiologically relevant substrate (5,7).  IDO2 function operates as a pro-inflammatory mediator in autoimmune inflammatory disorders (8, 9-11). It is a candidate for co-therapeutic targeting for treatment in these diseases (10-11).

References
  1. Prendergast, G.C. et al. (2014) Front. Immunol. 5:585.
  2. Witkiewicz, A.K. et al. (2009) J. Am. Coll. Surg. 208:781.
  3. Lob, S. et al. (2009) Cancer Immunol. Immunother. 58:153.
  4. Meininger, D. et al. (2011) Biochim. Biophys. Acta. 1814:1947.
  5. Pantouris, G. et al. (2014) Amino Acids 46:2155.
  6. Ball, H.J. et al. (2007) Gene 396:203.
  7. Yuasa, H.J. et al. (2009) Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 153:137.
  8. Metz, R. et al. (2014) Int. Immunol. 26:357.
  9. Merlo, L.M.F. et al. (2014) J. Immunol. 192:2082.
  10. Merlo, L.M. and L. Mandik-Nayak (2016) Clin. Med. Insights Pathol. 9:21.
  11. Merlo, L.M.F. et al (2017) Clin. Immunol. 179:8.
Long Name
Indoleamine 2,3-dioxygenase 2
Entrez Gene IDs
169355 (Human); 209176 (Mouse); 681319 (Rat)
Alternate Names
EC 1.13.11.-; IDO2; Ido-2; INDOL1; INDOL1indoleamine-pyrrole 2,3 dioxygenase-like 1; indoleamine 2,3-dioxygenase 2; Indoleamine 2,3-dioxygenase-2; indoleamine 2,3-dioxygenase-like 1 protein; Indoleamine 2,3-dioxygenase-like protein 1; Indoleamine-pyrrole 2,3-dioxygenase-like protein 1

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

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Reconstitution Buffers

Secondary Antibodies

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