Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1001802
View all IDO2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing  human IDO2
Met1-Val407
Accession # Q6ZQW0

Specificity

Detects human IDO2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human IDO2 Antibody

IDO2 antibody in Human Liver by Immunohistochemistry (IHC-P).

IDO2 in Human Liver.

IDO2 was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human IDO2 Monoclonal Antibody (Catalog # MAB10098) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human IDO2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Reviewed Applications

Read 1 review rated 5 using MAB10098 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: IDO2

Indoleamine 2,3-dioxygenase (IDO2) is a 47 kDa heme-containing cytosolic dioxygenase. Human IDO2 shares 64% aa sequence identity with mouse IDO2. IDO2 is one of three dioxygenases capable of catalyzing the first and rate-limiting step of the L-kynurenine pathway (KP): oxidative cleavage of the essential amino acid L-tryptophan to form N formyl kynurenine (1). Of these proteins, IDO1 and IDO2 are both related, monomeric enzymes but share only 38% aa sequence identity. The IDO isoforms are not functionally redundant. Although expression of IDO2 has been upregulated in some cancers (2,3), IDO2 expression is generally restricted to the liver, kidney, brain, and certain immune cell types unlike the more ubiquitously expressed indoleamine 2,3-dioxygenase (IDO) (1). Differential inhibition of IDO1 and IDO2 is observed with several molecules (4,5). IDO2 has significantly lower tryptophan catabolic activity than IDO1 and TDO2 (4, 6-8) suggesting it does not play a significant physiological role in the KP. Instead, IDO2 may have an alternative functional role: either non-enzymatic or utilizing a more physiologically relevant substrate (5,7).  IDO2 function operates as a pro-inflammatory mediator in autoimmune inflammatory disorders (8, 9-11). It is a candidate for co-therapeutic targeting for treatment in these diseases (10-11).

References

  1. Prendergast, G.C. et al. (2014) Front. Immunol. 5:585.
  2. Witkiewicz, A.K. et al. (2009) J. Am. Coll. Surg. 208:781.
  3. Lob, S. et al. (2009) Cancer Immunol. Immunother. 58:153.
  4. Meininger, D. et al. (2011) Biochim. Biophys. Acta. 1814:1947.
  5. Pantouris, G. et al. (2014) Amino Acids 46:2155.
  6. Ball, H.J. et al. (2007) Gene 396:203.
  7. Yuasa, H.J. et al. (2009) Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 153:137.
  8. Metz, R. et al. (2014) Int. Immunol. 26:357.
  9. Merlo, L.M.F. et al. (2014) J. Immunol. 192:2082.
  10. Merlo, L.M. and L. Mandik-Nayak (2016) Clin. Med. Insights Pathol. 9:21.
  11. Merlo, L.M.F. et al (2017) Clin. Immunol. 179:8.

Long Name

Indoleamine 2,3-dioxygenase 2

Alternate Names

Ido-2, INDOL1, Indoleamine 2,3-dioxygenase-2

Entrez Gene IDs

169355 (Human); 209176 (Mouse); 681319 (Rat)

Gene Symbol

IDO2

UniProt

Q6ZQW0

Additional IDO2 Products

Product Documents for Human IDO2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human IDO2 Antibody

For research use only

Customer Reviews for Human IDO2 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human IDO2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human IDO2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Liver tissue
    Species: Human
    Verified Customer | Posted 06/01/2022
    Human IDO2 Antibody MAB10098

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies