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Human IFN-beta Antibody

Catalog #: MAB814
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB814-SP
MAB814-100
MAB814-500

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IFN‑ beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ beta Antibody.
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Citations (7)
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Human IFN-beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human IFN-beta in Western blots. In Western blots, this antibody does not cross-react with recombinant human IFN‑ alpha.
Source
Monoclonal Mouse IgG1 Clone # 76703
Purification
Protein A or G purified from ascites
Immunogen
E. coli-derived recombinant human IFN-beta
Met22-Asn187
Accession # P01574
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IFN-beta
Flow Cytometry
0.25 µL/106 cells
M1 macrophages treated with 50 ng/mL each of rhGM-CSF (Catalog # 215-GM) for 6 days followed by an overnight polarization with IFNg and LPS ( Catalog # 285-IF) and restimulated with monensin for 3 hours (3uM)
Neutralization
Measured by its ability to neutralize IFN‑ beta inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 1.50-18.0 µg/mL in the presence of 50 pg/mL Recombinant Human IFN‑ beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization IFN‑ beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ beta Antibody. View Larger

IFN‑ beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ beta Antibody. Recombinant Human IFN-beta (8499-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-beta (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IFN-beta Monoclonal Antibody (Catalog # MAB814). The ND50 is typically 1.50-18.0 µg/mL.

Flow Cytometry View Larger

Detection of IFN‑ beta in M1 macrophages by Flow Cytometry. M1 macrophages treated with 50 ng/mL each of rhGM-CSF (215-GM) for 6 days followed by an overnight polarization with IFNg and LPS (285-IF) and restimulated with monensin for 3 hours (3uM) were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human IFN‑ beta Monoclonal Antibody (Catalog # MAB814) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-beta

Interferon beta (IFN-beta ) is synthesized and secreted by fibroblasts and many other cell types in response to pathogens. IFN-beta binding to type I interferon receptors induces the upregulation of IRF-7 and activation of Rnase L. IRF-7 can exert a positive feedback on IFN-beta production. RNase L cleaves both viral and cellular single stranded mRNA, thereby limiting viral replication and dissemination.

Long Name
Interferon beta
Entrez Gene IDs
3456 (Human); 15977 (Mouse); 24481 (Rat)
Alternate Names
Fibroblast interferon; IFB; IFBIFNB; IFF; IFNB; IFNB1; IFNbeta; IFN-beta; interferon beta; interferon, beta 1, fibroblast; MGC96956

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Citations for Human IFN-beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Epigenetic Repression of STING by MYC Promotes Immune Evasion and Resistance to Immune Checkpoint Inhibitors in Triple-Negative Breast Cancer
    Authors: Kyung-min Lee, Chang-Ching Lin, Alberto Servetto, Joonbeom Bae, Vishal Kandagatla, Dan Ye et al.
    Cancer Immunology Research
  2. Targeting KDM4A epigenetically activates tumor-cell-intrinsic immunity by inducing DNA replication stress
    Authors: Wuchang Zhang, Wei Liu, Lingfei Jia, Demeng Chen, Insoon Chang, Michael Lake et al.
    Molecular Cell
  3. Adipose Tissue-Derived Mesenchymal Stem Cells Suppress Growth of Huh7 Hepatocellular Carcinoma Cells via Interferon (IFN)-beta -Mediated JAK/STAT1 Pathway in vitro
    Authors: Chun Sung Byun, Soonjae Hwang, Sung-Hun Woo, Moon Young Kim, Jin Suk Lee, Jong In Lee et al.
    International Journal of Medical Sciences
  4. PUM1 is a biphasic negative regulator of innate immunity genes by suppressing LGP2
    Authors: Y Liu, L Qu, Y Liu, B Roizman, GG Zhou
    Proc. Natl. Acad. Sci. U.S.A., 2017-07-31;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Constitutively Active MAVS Inhibits HIV-1 Replication via Type I Interferon Secretion and Induction of HIV-1 Restriction Factors.
    Authors: Gupta S, Termini J, Issac B, Guirado E, Stone G
    PLoS ONE, 2016-02-05;11(2):e0148929.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Sialylation of Campylobacter jejuni endotoxin promotes dendritic cell-mediated B cell responses through CD14-dependent production of IFN-beta and TNF-alpha.
    Authors: Huizinga R, van Rijs W, Bajramovic J, Kuijf M, Laman J, Samsom J, Jacobs B
    J Immunol, 2013-10-28;191(11):5636-45.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neutralization
  7. An assessment of biological potency and molecular characteristics of different innovator and noninnovator interferon-beta products.
    Authors: Meager A, Dolman C, Dilger P, Bird C, Giovannoni G, Schellekens H, Thorpe R, Wadhwa M
    J. Interferon Cytokine Res., 2010-12-07;31(4):383-92.
    Species: Human
    Sample Types: Protein
    Applications: Western Blot

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