Human IFN-beta Antibody Summary
Accession # P01574
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IFN‑ beta Inhibition of EMCV-induced Cytopathy and Neutralization by Human IFN‑ beta Antibody. Recombinant Human IFN-beta (8499-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Human IFN-beta (50 pg/mL) is neutralized (green line) by increasing concentrations of Human IFN-beta Monoclonal Antibody (Catalog # MAB814). The ND50 is typically 1.50-18.0 µg/mL.
Detection of IFN‑ beta in M1 macrophages by Flow Cytometry. M1 macrophages treated with 50 ng/mL each of rhGM-CSF (215-GM) for 6 days followed by an overnight polarization with IFNg and LPS (285-IF) and restimulated with monensin for 3 hours (3uM) were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human IFN‑ beta Monoclonal Antibody (Catalog # MAB814) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Interferon beta (IFN-beta ) is synthesized and secreted by fibroblasts and many other cell types in response to pathogens. IFN-beta binding to type I interferon receptors induces the upregulation of IRF-7 and activation of Rnase L. IRF-7 can exert a positive feedback on IFN-beta production. RNase L cleaves both viral and cellular single stranded mRNA, thereby limiting viral replication and dissemination.
Citations for Human IFN-beta Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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PUM1 is a biphasic negative regulator of innate immunity genes by suppressing LGP2
Authors: Y Liu, L Qu, Y Liu, B Roizman, GG Zhou
Proc. Natl. Acad. Sci. U.S.A., 2017-07-31;0(0):.
Sample Types: Whole Cells
Constitutively Active MAVS Inhibits HIV-1 Replication via Type I Interferon Secretion and Induction of HIV-1 Restriction Factors.
Authors: Gupta S, Termini J, Issac B, Guirado E, Stone G
PLoS ONE, 2016-02-05;11(2):e0148929.
Sample Types: Whole Cells
Sialylation of Campylobacter jejuni endotoxin promotes dendritic cell-mediated B cell responses through CD14-dependent production of IFN-beta and TNF-alpha.
Authors: Huizinga R, van Rijs W, Bajramovic J, Kuijf M, Laman J, Samsom J, Jacobs B
J Immunol, 2013-10-28;191(11):5636-45.
Sample Types: Cell Culture Supernates
An assessment of biological potency and molecular characteristics of different innovator and noninnovator interferon-beta products.
Authors: Meager A, Dolman C, Dilger P, Bird C, Giovannoni G, Schellekens H, Thorpe R, Wadhwa M
J. Interferon Cytokine Res., 2010-12-07;31(4):383-92.
Sample Types: Protein
Applications: Western Blot
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