Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 991620
View all IFN-lambda 4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IFN-lambda 4
Ala23-Leu179
Accession # K9M1U5

Specificity

Detects human IFN-lambda 4 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human IFN-lambda 4 Antibody

IFN-?4 antibody in Human Small Intestine by Immunohistochemistry (IHC-P).

IFN-lambda 4 in Human Small Intestine.

IFN-lambda 4 was detected in immersion fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human IFN-lambda 4 Monoclonal Antibody (Catalog # MAB9165) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human IFN-lambda 4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spleen

Reviewed Applications

Read 1 review rated 5 using MAB9165 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-lambda 4

Interferon-lambda 4 (IFN-lambda 4) is a secreted, approximately 17 kDa member of the type III interferon family of molecules (1). It is generated by a frameshift mutation based on a TT > ∆G polymorphism (2). The TT allele inactivates expression of IFN-lambda 4 (2). The ∆G allele is associated with reduced clearance of hepatitis C virus and reduced degranulation of CTL, NK, and NKT cells in the liver during hepatitis (2, 3), although IFN- lambda 4 itself shows in vitro activity against hepatitis C virus (4). IFN-lambda 4 is expressed in the liver of some chronic hepatitis C patients (5). It signals through a receptor complex containing IL-28 R/IFN-lambda R1 and IL-10 R beta to induce the expression of several interferon stimulated genes (ISG) (2, 4). Alternative splicing generates additional isoforms with large internal deletions or a substituted C-terminal region.

References

  1. Wack, A. et al. (2015) Nat. Immunol. 16:802.
  2. Prokunina-Olsson, L. et al. (2013) Nat. Genet. 45:164.
  3. Jouvin-Marche, E. et al. (2014) J. Infec. Dis. 209:1907.
  4. Hamming, O.J. et al. (2013) EMBO J. 32:3055.
  5. Amanzada, A. et al. (2013) PLoS One 8:e84026.

Long Name

Interferon, Lambda 4

Alternate Names

IFNAN, IFNL4, IFNlambda 4

Entrez Gene IDs

101180976 (Human)

Gene Symbol

IFNL4

UniProt

K9M1U5

Additional IFN-lambda 4 Products

Product Documents for Human IFN-lambda 4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IFN-lambda 4 Antibody

For research use only

Related Research Areas

Interferons

Customer Reviews for Human IFN-lambda 4 Antibody (1)

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  • Human IFN-lambda 4 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Small intestine tissue
    Species: Human
    Verified Customer | Posted 09/10/2022
    Human IFN-lambda 4 Antibody MAB9165

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