Human IFN-lambda 4 Antibody
Human IFN-lambda 4 Antibody Summary
Accession # K9M1U5
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IFN-lambda 4 in Human Small Intestine. IFN-lambda 4 was detected in immersion fixed paraffin-embedded sections of human small intestine using Mouse Anti-Human IFN-lambda 4 Monoclonal Antibody (Catalog # MAB9165) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-lambda 4
Interferon-lambda 4 (IFN-lambda 4) is a secreted, approximately 17 kDa member of the type III interferon family of molecules (1). It is generated by a frameshift mutation based on a TT > ∆G polymorphism (2). The TT allele inactivates expression of IFN-lambda 4 (2). The ∆G allele is associated with reduced clearance of hepatitis C virus and reduced degranulation of CTL, NK, and NKT cells in the liver during hepatitis (2, 3), although IFN- lambda 4 itself shows in vitro activity against hepatitis C virus (4). IFN-lambda 4 is expressed in the liver of some chronic hepatitis C patients (5). It signals through a receptor complex containing IL-28 R/IFN-lambda R1 and IL-10 R beta to induce the expression of several interferon stimulated genes (ISG) (2, 4). Alternative splicing generates additional isoforms with large internal deletions or a substituted C-terminal region.
- Wack, A. et al. (2015) Nat. Immunol. 16:802.
- Prokunina-Olsson, L. et al. (2013) Nat. Genet. 45:164.
- Jouvin-Marche, E. et al. (2014) J. Infec. Dis. 209:1907.
- Hamming, O.J. et al. (2013) EMBO J. 32:3055.
- Amanzada, A. et al. (2013) PLoS One 8:e84026.
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