Human IGF-II R/IGF2R Antibody

Catalog # Availability Size / Price Qty
MAB2447
MAB2447-SP
IGF-II R/IGF2R in A172 Human Cell Line.
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Product Details
Citations (1)
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Reviews (1)

Human IGF-II R/IGF2R Antibody Summary

Species Reactivity
Human
Specificity
Detects human IGF-II R/IGF2R in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IGF‑I R/IGF1R is observed.
Source
Monoclonal Mouse IgG2B Clone # 372604
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IGF-II R/IGF2R
Ser1510-Phe2108
Accession # P11717.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Complete Your Experiment

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IGF-II R/IGF2R (Catalog # 2447-GR)
under non-reducing conditions only
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IGF-II R/IGF2R antibody in A172 Human Cell Line by Immunocytochemistry (ICC). View Larger

IGF-II R/IGF2R in A172 Human Cell Line. IGF-II R/IGF2R was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human IGF-II R/IGF2R Monoclonal Antibody (Catalog # MAB2447) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-II R/IGF2R

The type 2 insulin-like growth factor receptor (also known as cation-independent mannose-6 phosphate receptor/CI-MPR) is a 300 kDa member of the P-type lectin family of molecules. P-type lectins generate functional eukaryotic lysosomes by binding and sorting lysosomal enzymes expressing phosphorylated mannose residues (M6P) (1-3). IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment and a 124 aa cytoplasmic tail (4, 5). The extracellular region consists of 15 contiguous “binding” repeats of about 150 aa each. The odd-numbered repeats interact with “ligands” while the even-numbered repeats likely generate a nondisulfide homodimer in the membrane (1). Repeat #11 binds IGF-II, while repeats 3 and 9 bind mannose-6 phosphate; repeat #13 contains a fibronectin type II motif and assists in IGF-II binding (1, 2). In the extracellular region of IGF-II R expressed by R&D Systems (600 aa’s), human IGF-II R is 85% aa identical to both mouse and bovine IGF-II R. This expressed region includes binding repeats #11, 12, and 13. In addition to IGF-II, CI-MPR/IGF-II R binds non-M6P containing ligands such as retinoic acid, urokinase-type plasminogen-activator receptor and plasminogen, plus M6P-containing molecules such as lysosomal enzymes, TGF-beta 1 precursor, proliferin, LIF, CD26, herpes simplex glycoprotein D and granzymes A and B (2, 6). IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P-tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, it would appear that IGF-II R is principally a regulator of local IGF-II levels, targeting IGF-II for destruction in lysosomes (2). However, some evidence suggests the receptor will signal via G‑proteins, an effect that has yet to be conclusively shown (6).

References
  1. Ghosh, P. et al. (2003) Nat. Rev. Mol. Cell. Biol. 4:202.
  2. Dahms, N.M. and M.K. Hancock (2002) Biochim. Biophys. Acta. 1572:317.
  3. Zaina, S. and J. Nilsson (2003) Curr. Opin. Lipidol. 14:483.
  4. Morgan, D.O. et al. (1987) Nature 329:301.
  5. Oshima, A. et al. (1988) J. Biol. Chem. 263:2553.
  6. Hawkes, C. and S. Kar (2004) Brain Res. Rev. 44:117.
Long Name
Insulin-like Growth Factor II Receptor
Entrez Gene IDs
3482 (Human)
Alternate Names
300 kDa mannose 6-phosphate receptor; cation-independent mannose-6 phosphate receptor; cation-independent mannose-6-phosphate receptor; CD222 antigen; CD222; CI Man-6-P receptor; CIMPR; CI-MPR; IGF2R; IGF-II R; IGF-II receptor; IGFIIR; IGF-IIR; insulin-like growth factor 2 receptorM6P/IGF2R; Insulin-like growth factor II receptor; M6P/IGF2 receptor; M6PR; M6P-R; MPR 300; MPR1; MPRI; MPRIM6PR

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Citation for Human IGF-II R/IGF2R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Blockade of IGF2R improves muscle regeneration and ameliorates Duchenne muscular dystrophy
    Authors: P Bella, A Farini, S Banfi, D Parolini, N Tonna, M Meregalli, M Belicchi, S Erratico, P D'Ursi, F Bianco, M Legato, C Ruocco, C Sitzia, S Sangiorgi, C Villa, G D'Antona, L Milanesi, E Nisoli, P Mauri, Y Torrente
    EMBO Mol Med, 2019;0(0):e11019.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture

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Human IGF-II R MAb (Clone 372604)
By ZHIJIN CHEN on 04/15/2016
Application: WB Sample Tested: Cell Lysates Species: Human