Human IL-1 RII Antibody Summary
Phe14-Glu343 (Ser56Gly and Glu297Gly)
Accession # P27930
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
IL‑1 RII in Human PBMCs. IL‑1 RII was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 15 µg/mL Goat Anti-Human IL‑1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑263‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
IL‑1 RII Inhibition of IL‑1 beta /IL‑1F2-dependent Cell Proliferation and Neutralization by Human IL‑1 RII Antibody. Recombinant Human IL‑1 RII (Catalog # 263‑2R) inhibits Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201‑LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑1 beta /IL‑1F2 (50 pg/mL) activity elicited by Recombinant Human IL‑1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-263-NA). The ND50 is typically 15‑30 µg/mL in the presence of concanavalin A (1.25 µg/mL).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-1 RII
Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex.
An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.
Citations for Human IL-1 RII Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Ectopic expression of interleukin-1 receptor type II enhances cell migration through activation of the pre-interleukin 1alpha pathway.
Authors: Chang SY, Su PF, Lee TC
Sample Types: Whole Cells
Synergism of TNF and IL-1 in the induction of matrix metalloproteinase-3 in trabecular meshwork.
Authors: Kelley MJ, Rose AY, Song K, Chen Y, Bradley JM, Rookhuizen D, Acott TS
Invest. Ophthalmol. Vis. Sci., 2007;48(6):2634-43.
Sample Types: Cell Lysates
Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells.
Authors: Herrmann-Lavoie C, Rao CV, Akoum A
Sample Types: Cell Lysates
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MAB663 was used as the capture and AF263 was used as the detection antibody for the sandwich ELISA for sIL-1R2. The immunoassay standard was 263-2R. Assay had sensitivity of ~10pg/ml.