Human IL-1 RII Antibody

Catalog # Availability Size / Price Qty
AF-263-NA
AF-263-SP
IL‑1 RII in Human PBMCs.
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Product Details
Citations (3)
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Reviews (3)

Human IL-1 RII Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑1 RII in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) IL‑1 alpha, rhIL‑1 RI, recombinant mouse (rm) IL‑1 alpha, rhIL‑1 beta, rmIL‑1 beta, recombinant rat IL‑1 beta, rhIL‑1ra, rmIL‑1ra, and recombinant rhesus monkey IL‑1ra is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑1 RII
Phe14-Glu343 (Ser56Gly and Glu297Gly)
Accession # P27930
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑1 RII (Catalog # 263-2R)
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑1 RII-mediated inhibition of proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 15-30 µg/mL in the presence of 2 µg/mL Recombinant Human IL‑1 RII, 50 pg/mL Recombinant Human IL‑1 beta /IL‑1F2, and 1.25 µg/mL concanavalin A.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry IL‑1 RII in Human PBMCs. View Larger

IL‑1 RII in Human PBMCs. IL‑1 RII was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 15 µg/mL Goat Anti-Human IL‑1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑263‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization IL‑1 RII Inhibition of IL‑1 beta /IL‑1F2-dependent Cell Proliferation and Neutralization by Human IL‑1 RII Antibody. View Larger

IL‑1 RII Inhibition of IL‑1 beta /IL‑1F2-dependent Cell Proliferation and Neutralization by Human IL‑1 RII Antibody. Recombinant Human IL‑1 RII (Catalog # 263‑2R) inhibits Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201‑LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL‑1 beta /IL‑1F2 (50 pg/mL) activity elicited by Recombinant Human IL‑1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-263-NA). The ND50 is typically 15‑30 µg/mL in the presence of concanavalin A (1.25 µg/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-1 RII

Two distinct types of receptors that bind the pleiotropic cytokines IL-1 alpha and IL-1 beta have been described. The IL-1 receptor type I is an 80 kDa transmembrane protein that is expressed predominantly by T cells, fibroblasts and endothelial cells. IL-1 receptor type II is a 68 kDa transmembrane protein found on B lymphocytes, neutrophils, monocytes, large granular leukocytes and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show approximately 28% sequence similarity in their extracellular domains. The two receptor types do not heterodimerize in a receptor complex.

An IL-1 receptor accessory protein that can heterodimerize with the type I receptor in the presence of IL-1 alpha or IL-1 beta but not IL-1ra, was identified (1). This type I receptor complex appears to mediate all the known IL-1 biological responses. The receptor type II has a short cytoplasmic domain and does not transduce IL-1 signals. In addition to the membrane-bound form of IL-1 RII, a naturally-occurring soluble form of IL-1 RII has been described. It has been suggested that the type II receptor, either as the membrane-bound or as the soluble form, serves as a decoy for IL-1 and inhibits IL-1 action by blocking the binding of IL-1 to the signaling type I receptor complex. Recombinant IL-1 soluble receptor type II is a potent antagonist of IL-1 action.

References
  1. Greenfeder, S. et al. (1995) J. Biol. Chem. 270:13757.
Long Name
Interleukin 1 Receptor II
Entrez Gene IDs
7850 (Human); 16178 (Mouse)
Alternate Names
beta; CD121b antigen; CD121b; IL-1 R beta; IL-1 RII; IL1R2; IL1RBCD121 antigen-like family member B; IL-1R-beta; IL1RII; IL-1RII; IL-1RT2; IL-1RT-2; interleukin 1 receptor, type II; Interleukin-1 receptor beta; MGC47725

Product Datasheets

Citations for Human IL-1 RII Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Ectopic expression of interleukin-1 receptor type II enhances cell migration through activation of the pre-interleukin 1alpha pathway.
    Authors: Chang SY, Su PF, Lee TC
    Cytokine, 2009;45(1):32-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Synergism of TNF and IL-1 in the induction of matrix metalloproteinase-3 in trabecular meshwork.
    Authors: Kelley MJ, Rose AY, Song K, Chen Y, Bradley JM, Rookhuizen D, Acott TS
    Invest. Ophthalmol. Vis. Sci., 2007;48(6):2634-43.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  3. Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells.
    Authors: Herrmann-Lavoie C, Rao CV, Akoum A
    Endocrinology, 2007;148(11):5377-84.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

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Reviews for Human IL-1 RII Antibody

Average Rating: 4.7 (Based on 3 Reviews)

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Human IL-1 RII Antibody
By Anonymous on 05/02/2018
Application: WB Sample Tested: Pancreatic cancer cells Species: Human

Human IL‑1 RII Antibody
By Anonymous on 03/23/2018
Application: WB Sample Tested: Human recombinant test antibody Species: Human

Human IL-1 RII Antibody
By Anonymous on 11/09/2017
Application: ELISA Sample Tested: Serum and Plasma,Cell culture supernatant Species: Human

MAB663 was used as the capture and AF263 was used as the detection antibody for the sandwich ELISA for sIL-1R2. The immunoassay standard was 263-2R. Assay had sensitivity of ~10pg/ml.