Human IL-10 R alpha Antibody

Catalog # Availability Size / Price Qty

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

IL‑10 R alpha  in Human PBMCs.
2 Images
Product Details
Citations (2)
Supplemental Products

Human IL-10 R alpha Antibody Summary

Species Reactivity
Detects human IL‑10 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse
IL-10 R alpha is observed and less than 5% cross-reactivity with recombinant human (rh) CNTF sR alpha and rhIL-2 sR gamma is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑10 R alpha
Accession # Q13651
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human IL‑10 R alpha (Catalog # 274-R1)
5-15 µg/mL
See below
Measured by its ability to neutralize IL‑10 R alpha -mediated IL‑10 response in LPS-activated human peripheral blood mononuclear cells (PBMC) [Ralph, P. et al. (1991) J. Immunology 148:808]. The Neutralization Dose (ND50) is typically 20-40 µg/mL in the presence of 0.25 ng/mL Recombinant Human IL‑10 and 0.25-3 ng/mL LPS.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry IL‑10 Ra antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

IL‑10 R alpha in Human PBMCs. IL‑10 R alpha was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 15 µg/mL Goat Anti-Human IL‑10 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑274‑NA) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization IL‑10 Inhibition of IL‑1 beta  secretion and Neutralization by Human IL‑10 R alpha  Antibody. View Larger

IL‑10 Inhibition of IL‑1 beta secretion and Neutralization by Human IL‑10 R alpha Antibody. Recombinant Human IL-10 (Catalog # 217-IL) inhibits IL-1 beta secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL-1 beta /IL-1F2 Quantikine ELISA Kit (Catalog # DLB50). IL-1 beta secretion inhibited by Recombinant Human IL-10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-10 Ra Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-274-NA). The ND50 is typically 20-40 µg/mL in the presence of LPS (0.25-3 ng/mL).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10 R alpha

IL-10, initially designated cytokine synthesis inhibitory factor (CSIF), is a potent immunosuppressant of macrophage functions. IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. IL-10 binds specifically and with high affinity to cell-surface receptors. Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. The IL-10 R mRNA has been detected in all cell types that are known to respond to IL-10.

Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. These receptors are members of the class II subgroup of the cytokine receptor superfamily. The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. The human IL-10 R gene has been mapped to chromosome 11q23.3. Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist.

Long Name
Interleukin 10 Receptor alpha
Entrez Gene IDs
3587 (Human); 16154 (Mouse); 102127536 (Cynomolgus Monkey)
Alternate Names
CD210 antigen; CDw210a; HIL-10R; IBD28; IL-10 R alpha; IL-10 receptor subunit alpha; IL10R alpha; IL-10R subunit 1; IL-10R1; IL10RA; IL-10Ra; IL10RIL-10R subunit alpha; interleukin 10 receptor, alpha; interleukin-10 receptor alpha chain; Interleukin-10 receptor subunit 1; interleukin-10 receptor subunit alpha

Product Datasheets

You must select a language.


Citations for Human IL-10 R alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

  1. Multi-Step Regulation of the TLR4 Pathway by the miR-125a~99b~let-7e Cluster
    Authors: Graziella Curtale, Tiziana A. Renzi, Massimiliano Mirolo, Lorenzo Drufuca, Manuel Albanese, Mariacristina De Luca et al.
    Frontiers in Immunology
  2. Differential regulation of CD44 expression by lipopolysaccharide (LPS) and TNF-alpha in human monocytic cells: distinct involvement of c-Jun N-terminal kinase in LPS-induced CD44 expression.
    Authors: Gee K, Lim W, Ma W, Nandan D, Diaz-Mitoma F, Kozlowski M, Kumar A
    J. Immunol., 2002-11-15;169(10):5660-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization


  1. Immunoprecipitaiton with the Human IL-10 Rα antibody (Catalog # AF-274-NA) followed by western blotting for Human IL-10 Rα produces a 150 kDa band. What is the identity of this band?

    • Human IL-10 Rα antibody has not been validated for immunoprecipitaiton application. The MW of Human IL-10 Rα is about 90-110 kDa and the receptor binds with high affinity to the IL-10 homodimer, which has a MW of 40 kDa. Under non-denaturing conditions, the IL-10Rα may be complexed with IL-10 homodimer to give a 150 kDa band.

View all Antibody FAQs

Reviews for Human IL-10 R alpha Antibody

There are currently no reviews for this product. Be the first to review Human IL-10 R alpha Antibody and earn rewards!

Have you used Human IL-10 R alpha Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review