Human IL-15 Antibody

(10 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-15 in Western blots. In Western blots, no cross-reactivity with recombinant human IL‑2 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 34593
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    E. coli-derived recombinant human IL-15
    Asn49-Ser162
    Accession # P40933
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human IL‑15 (Catalog # 247-ILB)
  • Neutralization
    Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 0.007-0.042 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑15.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody. Recombinant Human IL‑15 (Catalog # 247-ILB) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL‑15 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑15 Monoclonal Antibody (Catalog # MAB247). The ND50 is typically
0.007-0.042 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-15
Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).
  • References:
    1. Grabstein, K. et al. (1994) Science 264:965.
    2. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
    3. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
    4. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
    5. Giri, J.G. et al. (1995) EMBO 14:3654.
    6. Giri, J. et al. (1994) EMBO J. 13:2822.
    7. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
    8. Dubois, S. et al. (2002) Immunity 17:537.
    9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
    10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
    11. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
    12. Mortier, E. et al. (2004) J. Immunol. 173:1681.
    13. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
    14. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
    15. Neely, G.G. et al. (2004) J. Immunol. 172:4225.
  • Long Name:
    Interleukin 15
  • Entrez Gene IDs:
    3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
  • Alternate Names:
    IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum.
    Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y
    J Biol Chem, 2015;290(37):22338-51.
    Species: Human
    Sample Type: Protein
    Application: Neut
  2. IL-15 Expression on RA Synovial Fibroblasts Promotes B Cell Survival.
    Authors: Benito-Miguel M, Garcia-Carmona Y, Balsa A, Bautista-Caro MB, Arroyo-Villa I, Cobo-Ibanez T, Bonilla-Hernan MG, De Ayala CP, Sanchez-Mateos P, Martin-Mola E, Miranda-Carus ME
    PLoS ONE, 2012;7(7):e40620.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Development of an IL-15-autocrine CD8 T-cell leukemia in IL-15-transgenic mice requires the cis expression of IL-15R alpha.
    Authors: Sato N, Sabzevari H, Fu S, Ju W, Petrus MN, Bamford RN, Waldmann TA, Tagaya Y
    Blood, 2011;117(15):4032-40.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  4. TLR3 and Rig-like receptor on myeloid dendritic cells and Rig-like receptor on human NK cells are both mandatory for production of IFN-gamma in response to double-stranded RNA.
    Authors: Perrot I, Deauvieau F, Massacrier C
    J. Immunol., 2010;185(4):2080-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  5. Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
    Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
    Arthritis Rheum., 2006;54(7):2096-108.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Cloning and expression of feline interleukin 15.
    Authors: Dean GA, Barger A, Lavoy A
    Cytokine, 2005;29(2):77-83.
    Species: Feline
    Sample Type: Whole Cells
    Application: ICC
  7. Distinct roles of IL-12 and IL-15 in human natural killer cell activation by dendritic cells from secondary lymphoid organs.
    Authors: Ferlazzo G, Pack M, Thomas D, Paludan C, Schmid D, Strowig T, Bougras G, Muller WA, Moretta L, Munz C
    Proc. Natl. Acad. Sci. U.S.A., 2004;101(47):16606-11.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. IL-15 and the initiation of cell contact-dependent synovial fibroblast-T lymphocyte cross-talk in rheumatoid arthritis: effect of methotrexate.
    Authors: Miranda-Carus ME, Balsa A, Benito-Miguel M, Perez de Ayala C, Martin-Mola E
    J. Immunol., 2004;173(2):1463-76.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. Fibroblasts from human spleen regulate NK cell differentiation from blood CD34(+) progenitors via cell surface IL-15.
    Authors: Briard D, Brouty-Boye D, Azzarone B, Jasmin C
    J. Immunol., 2002;168(9):4326-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  10. Regulatory activity of autocrine IL-10 on dendritic cell functions.
    Authors: Corinti S, la Sala A, Pastore S
    J. Immunol., 2001;166(7):4312-8.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
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