Human IL-15 Antibody (34593)

R&D Systems | Catalog # MAB247

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Feline

Applications

Validated:

Western Blot, Neutralization

Cited:

Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Bioassay, ELISA Capture, ELISA Development, Immunogold Electron Microscopy

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 34593
View all IL-15 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IL-15
Asn49-Ser162
Accession # P40933

Specificity

Detects human IL-15 in Western blots. In Western blots, no cross-reactivity with recombinant human IL‑2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human IL-15 Antibody (34593)

Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody.

Cell Proliferation Induced by IL‑15 and Neutralization by Human IL‑15 Antibody.

Recombinant Human IL-15 (Catalog # 247-ILB) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-15 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-15 Monoclonal Antibody (Catalog # MAB247). The ND50 is typically 0.007-0.042 µg/mL.
Human IL-15 ELISA Standard Curve

Human IL-15 ELISA Standard Curve

Human BMP-7 was serially diluted and captured by Mouse Anti-Human/Primate IL‑15 Monoclonal Antibody (Catalog # MAB647) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL‑15 Monoclonal Antibody (Catalog # MAB247) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)
Detection of IL-15 by Immunohistochemistry

Detection of IL-15 by Immunohistochemistry

In vivo experiments confirmed that tumor-derived NOX4 inhibit the apoptosis of GC cells by recruiting neutrophils into the tumor microenvironment through GM-CSF. (A) Tumor formation and treatment model of NOD/SCID mice. (B-C) NOD/SCID mice (6 per group) were inoculated subcutaneously with NOX4-overexpression MKN45 GC cell. Once tumor size reached 2mm3, 5×106/200μL of neutrophils from healthy donors and pre-treated with different chemokine neutralizing antibodies (anti-CD14, anti-GM-CSF and anti-DKK1, respectively) were injected into the tail vein. Tumor growth curves (C) and tumor burdens (D) plotted from data measured every 4 days. Data points are shown only up to the time point at which the average tumor volume in any group first reached the predefined ethical limit. Mice were euthanized thereafter. (E) Neutrophils infiltration in tumors determined by CD66b IHC in each group. The red arrow refers to neutrophils. (F) Apoptosis of gastric cancer cells determined by Tunel Apoptosis Detection Kit in each group. Data are presented as the mean ± SD. *P < 0.05; **P < 0.01; ***P< 0.001. Image collected and cropped by CiteAb from the following open publication (https://www.ijbs.com/v22p4919.htm), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human IL-15 Antibody (34593)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human IL‑15 (Catalog # 247-ILB)

Neutralization

Measured by its ability to neutralize IL‑15-induced proliferation in the MO7e human megakaryocytic leukemic cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND50) is typically 0.007-0.042 µg/mL in the presence of 10 ng/mL Recombinant Human IL‑15.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-15

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).

References

  1. Grabstein, K. et al. (1994) Science 264:965.
  2. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259.
  3. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657.
  4. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
  5. Giri, J.G. et al. (1995) EMBO 14:3654.
  6. Giri, J. et al. (1994) EMBO J. 13:2822.
  7. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851.
  8. Dubois, S. et al. (2002) Immunity 17:537.
  9. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85.
  10. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  12. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  13. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167.
  14. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  15. Neely, G.G. et al. (2004) J. Immunol. 172:4225.

Long Name

Interleukin 15

Alternate Names

IL15

Entrez Gene IDs

3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)

Gene Symbol

IL15

UniProt

P40933

Additional IL-15 Products

Product Documents for Human IL-15 Antibody (34593)

Certificate of Analysis

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Product Specific Notices for Human IL-15 Antibody (34593)

For research use only

Citations for Human IL-15 Antibody (34593)

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