Human IL-17 RA/IL-17 R Antibody

(12 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed
  • Source
    Monoclonal Mouse IgG1 Clone # 133617
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 R
    Leu33-Trp320
    Accession # Q96F46
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human IL‑17 RA/IL‑17 R Fc Chimera (Catalog # 177-IR)
  • Flow Cytometry
    2.5 µg/106 cells
    K562 human chronic myelogenous leukemia cell line
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize IL‑17/IL‑17A-induced CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line. The Neutralization Dose (ND50) is typically 0.4-2 μg/mL in the presence of 40 ng/mL Recombinant Human IL‑17/IL‑17A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

CXCL1/GRO alpha Secretion Induced by IL‑17/IL‑17A and Neutralization by Human IL‑17 RA/IL‑17 R Antibody. Recombinant Human IL‑17/IL‑17A induces CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GRO alpha DuoSet (Catalog # DY275). CXCL1/GRO alpha secretion elicited by Recombinant Human IL‑17/IL‑17A (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑17 RA/IL‑17 R Monoclonal Antibody (Catalog # MAB177). The ND50 is typically 0.4-2 μg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17 RA/IL-17 R

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.

  • References:
    1. Yao, Z. et al. (1997) Cytokine 9:794.
    2. Schwander, R. et al. (2000) J. Exp. Med. 191:1233.
  • Long Name:
    Interleukin 17 Receptor
  • Entrez Gene IDs:
    23765 (Human); 16172 (Mouse); 312679 (Rat)
  • Alternate Names:
    CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 RA; IL-17 receptor A; IL17RA; IL-17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Rapid and Rigorous IL-17A Production by a Distinct Subpopulation of Effector Memory T Lymphocytes Constitutes a Novel Mechanism of Toxic Shock Syndrome Immunopathology
    Authors: PA Szabo, A Goswami, DM Mazzuca, K Kim, DB O'Gorman, DA Hess, ID Welch, HA Young, B Singh, JK McCormick, SM Haeryfar
    J. Immunol, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  2. Autophagy impacts on oxaliplatin-induced hepatocarcinoma apoptosis via the IL-17/IL-17R-JAK2/STAT3 signaling pathway
    Authors: J Wu, J Guo, Q Cao, Y Wang, J Chen, Z Wang, Z Yuan
    Oncol Lett, 2017;13(2):770-776.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  3. Interleukin 17 inhibits progenitor cells in rheumatoid arthritis cartilage.
    Authors: Schminke B, Trautmann S, Mai B, Miosge N, Blaschke S
    Eur J Immunol, 2015;46(2):440-5.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. T-helper cell type 2 (Th2) memory T cell-potentiating cytokine IL-25 has the potential to promote angiogenesis in asthma.
    Authors: Corrigan CJ, Wang W, Meng Q, Fang C, Wu H, Reay V, Lv Z, Fan Y, An Y, Wang YH, Liu YJ, Lee TH, Ying S
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(4):1579-84.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. IL-17C regulates the innate immune function of epithelial cells in an autocrine manner.
    Authors: Ramirez-Carrozzi V, Sambandam A, Luis E
    Nat. Immunol., 2011;12(12):1159-66.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. TH17 cytokines induce human airway smooth muscle cell migration.
    Authors: Chang Y, Al-Alwan L, Risse PA, Roussel L, Rousseau S, Halayko AJ, Martin JG, Hamid Q, Eidelman DH
    J. Allergy Clin. Immunol., 2011;127(4):1046-53.e1-2.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  7. Functional coexpression of Interleukin (IL)-7 and its receptor (IL-7R) on Hodgkin and Reed-Sternberg cells: Involvement of IL-7 in tumor cell growth and microenvironmental interactions of Hodgkin's lymphoma.
    Authors: Cattaruzza L, Gloghini A, Olivo K, Di Francia R, Lorenzon D, De Filippi R, Carbone A, Colombatti A, Pinto A, Aldinucci D
    Int. J. Cancer, 2009;125(5):1092-101.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. IL-17 induces monocyte migration in rheumatoid arthritis.
    Authors: Shahrara S, Pickens SR, Dorfleutner A, Pope RM
    J. Immunol., 2009;182(6):3884-91.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  9. Interleukin-17 and interferon-gamma are produced concomitantly by human coronary artery-infiltrating T cells and act synergistically on vascular smooth muscle cells.
    Authors: Eid RE, Rao DA, Zhou J, Lo SF, Ranjbaran H, Gallo A, Sokol SI, Pfau S, Pober JS, Tellides G
    Circulation, 2009;119(10):1424-32.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  10. CD molecules 2006--human cell differentiation molecules.
    Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X
    J. Immunol. Methods, 2007;319(1):1-5.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
    Authors: Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
    J. Immunol., 2005;175(9):6177-89.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
    Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
    Mucosal Immunol, 0;5(2):140-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 12 Citations
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

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Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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