Detects human IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL‑17 R, rmIL‑17B R, or recombinant human IL‑17B R is observed. In Western blots, no cross-reactivity with rmIL-17 R is observed
Monoclonal Mouse IgG1 Clone # 133617
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human IL‑17 RA/IL‑17 R Leu33-Trp320 Accession # Q96F46
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human IL‑17 RA/IL‑17 R Fc Chimera (Catalog # 177-IR)
2.5 µg/106 cells
K562 human chronic myelogenous leukemia cell line
Measured by its ability to neutralize IL‑17/IL‑17A-induced CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line. The Neutralization Dose (ND50) is typically 0.4-2 μg/mL in the presence of 40 ng/mL Recombinant Human IL‑17/IL‑17A.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
CXCL1/GRO alpha Secretion Induced by IL‑17/IL‑17A and Neutralization by Human IL‑17 RA/IL‑17 R Antibody.
Recombinant Human IL‑17/IL‑17A induces CXCL1/GRO alpha secretion in the HT‑29 human colon adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human CXCL1/GRO alpha DuoSet (Catalog # DY275). CXCL1/GRO alpha secretion elicited by Recombinant Human IL‑17/IL‑17A (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‑17 RA/IL‑17 R Monoclonal Antibody (Catalog # MAB177). The ND50 is typically 0.4-2 μg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-17 RA/IL-17 R
Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleotropic cytokine. IL-17 binds to IL-17 receptor (IL-17 R) which shares no homology with any known family of receptors. While the expression of IL-17 is restricted to activated T cells, the IL-17 R mRNA exhibits a broad tissue distribution, and has been detected in virtually all cells and tissues tested. Human IL-17 R is a 120 kDa, 866 amino acid (aa) type I membrane glycoprotein with a 293 aa extracellular domain, a 21 aa carboxy-proximal transmembrane domain, and a 525 aa cytoplasmic tail. Within the ECD, human IL-17 R shares 72% aa sequence identity with mouse and rat IL‑17 R. The signaling events of IL-17 includes activation of NF-kappa B and JNK, and require TNF receptor-associated factors 6 (TRAF6) in the signaling pathway.
Yao, Z. et al. (1997) Cytokine 9:794.
Schwander, R. et al. (2000) J. Exp. Med. 191:1233.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
CD molecules 2006--human cell differentiation molecules. Authors: Zola H, Swart B, Banham A, Barry S, Beare A, Bensussan A, Boumsell L, D Buckley C, Buhring HJ, Clark G, Engel P, Fox D, Jin BQ, Macardle PJ, Malavasi F, Mason D, Stockinger H, Yang X J. Immunol. Methods, 2007;319(1):1-5. Species: Human Sample Type: Whole Cells Application: Flow
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