Human IL-17RB Antibody

Catalog # Availability Size / Price Qty
AF1207
AF1207-SP
Human IL-17RB Antibody in Flow Cytometry
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Product Details
Citations (1)
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Human IL-17RB Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL‑17 RB in Western blots. In Western blots, approximately 35% cross-reactivity with recombinant mouse (rm) IL‑17 RB is observed, approximately 5% cross-reactivity with rmIL-17 R is observed, and less than 1% cross-reactivity with recombinant human IL‑17 R is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human IL‑17 RB
Arg18-Gly289
Accession # NP_061195
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human IL‑17 RB Fc Chimera (Catalog # 1207-BR)
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of IL‑17 RB in K562 Human Cell Line by Flow Cytometry. View Larger

Detection of IL‑17 RB in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Goat Anti-Human IL-17 RB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1207, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunohistochemistry IL‑17 RB in Human Kidney. View Larger

IL‑17 RB in Human Kidney. IL-17 RB was detected in immersion fixed paraffin-embedded sections of human kidney using 15 µg/mL Goat Anti-Human IL-17 RB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1207) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17RB

The interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These pro-inflammatory cytokines can induce local cytokine productions and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1).

Interleukin-17 B Receptor (IL-17B R), also known as IL-17Rh1, IL-17E R. and EVI27, represents the second receptor of the IL-17 family of cytokines to be recognized (2-4). Human IL-17B R cDNA encodes a 502 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 275 aa extracellular domain, a 21 aa transmembrane domain, and a 189 aa cytoplasmic tail. By alternative splicing, a secreted variant of IL-17B R has also been identified (4). Human and mouse IL‑17B R share 76% aa sequence identity. The human IL-17B R protein sequence is only 19.2% identical to the human IL-17 R sequence, but the two receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. Three additional type I transmembrane receptors with homology to IL-17 R have been reported, increasing the number of the IL-17 R family members to five (5, 6). By Northern blot analysis, human IL-17B R is highly expressed in kidneys and liver but is expressed at lower levels in testes, brain, small intestine and other endocrine tissues (2-4). The expression of IL-17B R is significantly up-regulated under inflammatory conditions. IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17, IL-17C, and IL-17F. Activation of IL-17B R by its ligands results in the activation of nuclear factor kappa-B (2-4).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Shi Y, et al. (2000) J. Biol. Chem. 275:19167.
  3. Lee, J, et al. (2001) J. Biol. Chem. 276:1660.
  4. Tian E, et al. (2000) Oncogene 19:2098.
  5. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  6. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
Long Name
Interleukin 17B Receptor
Entrez Gene IDs
55540 (Human); 50905 (Mouse)
Alternate Names
CRL4IL-17 receptor homolog 1; cytokine receptor CRL4; Cytokine receptor-like 4; Evi27; EVI27IL-17B receptor; IL-17 RB; IL-17 receptor B; IL-17 RH1; IL17BRinterleukin 17B receptor; IL-17ER; IL17RB; IL-17RB; IL17Rh1; IL-17Rh1; IL17RH1MGC5245; interleukin 17 receptor B; interleukin 17 receptor homolog 1; interleukin-17 receptor B; Interleukin-17B receptor

Product Datasheets

Citation for Human IL-17RB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Interleukin-25-induced chemokines and interleukin-6 release from eosinophils is mediated by p38 mitogen-activated protein kinase, c-Jun N-terminal kinase, and nuclear factor-kappaB.
    Authors: Wong CK, Cheung PF, Ip WK, Lam CW
    Am. J. Respir. Cell Mol. Biol., 2005;33(2):186-94.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

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Secondary Antibodies

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