Recombinant Human IL‑18 R alpha /IL‑1 R5 Fc Chimera (Catalog # 816-LR)
2.5 µg/106 cells
Human CD3+ T cells treated with PHA and Recombinant Human IL‑2 (Catalog # 202-IL)
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑18/IL‑1F4.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
IL‑18 R alpha /IL‑1 R5 in Human Skin.
IL‑18 R alpha /IL‑1 R5 was detected in immersion fixed frozen sections of human skin using Goat Anti-Human IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Human IL‑18 R alpha /IL‑1 R5 Antibody.
Recombinant Human IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line, in a dose-dependent manner (orange line), as measured by the Human IFN‑ gamma Quantikine ELISA Kit (Catalog # DIF50). IFN‑ gamma secretion elicited by IL‑18/IL‑1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840). The ND50 is typically 0.1-0.4 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-18 R alpha/IL-1 R5
Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL‑18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate, and placenta.
Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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