Human IL-18 R alpha /IL-1 R5 Antibody

(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IL‑18 R alpha /IL-1 R5 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse IL‑18 R alpha is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5
    Glu20-Arg329
    Accession # Q13478
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human IL‑18 R alpha /IL‑1 R5 Fc Chimera (Catalog # 816-LR)
  • Flow Cytometry
    2.5 µg/106 cells
    Human CD3+ T cells treated with PHA and Recombinant Human IL‑2 (Catalog # 202-IL)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑18/IL‑1F4.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
IL‑18 R alpha /IL‑1 R5 in Human Skin. IL‑18 R alpha /IL‑1 R5 was detected in immersion fixed frozen sections of human skin using Goat Anti-Human IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of keratinocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by Human IL‑18 R alpha /IL‑1 R5 Antibody. Recombinant Human IL‑18/IL‑1F4 stimulates IFN‑ gamma secretion in the KG‑1 human acute myelogenous leukemia cell line, in a dose-dependent manner (orange line), as measured by the Human IFN‑ gamma Quantikine ELISA Kit (Catalog # DIF50). IFN‑ gamma secretion elicited by IL‑18/IL‑1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑18 R alpha /IL‑1 R5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF840). The ND50 is typically 0.1-0.4 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-18 R alpha/IL-1 R5

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immuno-regulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low-affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL-18 R alpha and IL‑18 R beta is required for high-affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa residues. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate, and placenta.

  • References:
    1. Parnet, P. et al. (1996) J. Biol. Chem. 271:3967.
    2. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737.
    3. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
  • Long Name:
    Interleukin 18 Receptor alpha
  • Entrez Gene IDs:
    8809 (Human); 16182 (Mouse)
  • Alternate Names:
    CD218a; IL-1 R5; IL-1 Rrp; IL-18 R alpha; IL-18 R1; IL18R alpha; IL18R1; IL-18Ra
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. MAIT cells reside in the female genital mucosa and are biased towards IL-17 and IL-22 production in response to bacterial stimulation
    Authors: A Gibbs, E Leeansyah, A Introini, D Paquin-Pro, K Hasselrot, E Andersson, K Broliden, JK Sandberg, A Tjernlund
    Mucosal Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Whole Tissue
    Application: IF
  2. Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma
    Sci Rep, 2016;6(0):26757.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells.
    Authors: Dusseaux M, Martin E, Serriari N, Peguillet I, Premel V, Louis D, Milder M, Le Bourhis L, Soudais C, Treiner E, Lantz O
    Blood, 2011;117(4):1250-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Differentiation of human SH-SY5Y neuroblastoma cells by all-trans retinoic acid activates the interleukin-18 system.
    Authors: Sallmon H, Hoene V, Weber SC, Dame C
    J. Interferon Cytokine Res., 2010;30(2):55-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Caspase-1-processed interleukins in hyperoxia-induced cell death in the developing brain.
    Authors: Felderhoff-Mueser U, Sifringer M, Polley O, Dzietko M, Leineweber B, Mahler L, Baier M, Bittigau P, Obladen M, Ikonomidou C, Buhrer C
    Ann. Neurol., 2005;57(1):50-9.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
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