Human IL-23R Antibody

Catalog #: MAB14001
9 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB14001
MAB14001-SP

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Detection of Human IL-23 R by Western Blot.

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All IL-23R Antibodies

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Citations (9)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human IL-23R Antibody Summary

Species Reactivity

Human

Specificity

Detects human IL-23 R in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) IL‑23 R.

Source

Monoclonal Mouse IgG_2B Clone # 218213

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human IL-23 R
Gly24-Ile354
Accession # Q5VWK5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human IL-23 R by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Human IL-23 R Monoclonal Antibody (Catalog # MAB14001) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-23 R at approximately 58 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-23R

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12 (1 - 5). The functional IL-23 receptor complex consists of two receptor subunits, the IL-12 receptor beta 1 subunit (IL-12 R beta 1) and the IL-23-specific receptor subunit (IL-23 R) (3). Human IL-23 R cDNA encodes a 629 aa type I transmembrane protein with a 23 aa residue signal peptide, a 330 aa residue extracellular domain, a 23 aa residue transmembrane domain and a 253 aa residue cytoplasmic region. IL-23 R shares structural features with the IL-12 R beta 2, including an N-terminal Ig-like domain, two cytokine receptor domains and multiple glycosylation sites in the extracellular domain. IL-23 R lacks the three extracellular membrane-proximal fibronectin-type III domains present on IL-12 R beta 2. IL-23 R has a WQPWS sequence in the transmembrane-proximal cytokine receptor domain similar to the cytokine receptor signature WSXWS motif. The cytoplasmic region of IL-23 R has three potential Src homology 2 domain-binding sites and two potential Stat-binding sites. The gene for human IL-23 R is located on human chromosome 1 within 150 kb of IL-12 R beta 2. Human and mouse IL-23 R share 66% amino acid sequence identity. Based on quantitative real-time PCR, human IL-23 R mRNA is expressed in a human Th1 and Th0 clone as well as several NK cell lines and clones. Low but detectable levels of IL-23 R mRNA is also expressed in EBV-transformed B cells and activated PBMC. IL-23 initiates a signal transduction cascade similar to that of IL-12, and involves Jak2, Tyk2, Stat1, Stat3, Stat4, and Stat5. IL-23 has biological activities that are similar to, but distinct from IL-12.

References

Oppmann, B. et al. (2000) Immunity 13:715.
Lankford, C.S. and D.M. Frucht (2003) J. Leukoc. Biol. 73:49.
Parham, C. et al. (2002) J. Immunol. 168:5448.
Belladonna, M.L. et al. (2002) J. Immunol. 168:5448.
Aggarwal, S. et al. (2003) J. Biol. Chem. 278:1910.

Long Name

Interleukin 23 Receptor

Entrez Gene IDs

149233 (Human); 209590 (Mouse); 102133210 (Cynomolgus Monkey)

Alternate Names

IBD17; IL-23 R; IL-23 receptor; IL23R; IL-23R; interleukin 23 receptor; interleukin-23 receptor

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human IL-23R Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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IL23 Promotes Antimicrobial Pathways in Human Macrophages, Which Are Reduced With the IBD-Protective IL23R R381Q Variant
Authors: R Sun, C Abraham
Cell Mol Gastroenterol Hepatol, 2020-05-29;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Prognostic value of serum soluble interleukin-23 receptor and related T-helper 17 cell cytokines in non-small cell lung carcinoma
Authors: D Liu, S Xing, W Wang, X Huang, H Lin, Y Chen, K Lan, L Chen, F Luo, S Qin, R Liang, C Bai, J Xu, W Liu
Cancer Sci., 2020-03-04;111(4):1093-1102.
Species: Human
Sample Types: Tissue
Applications: ELISA (Capture), IHC
Th17-related cytokines contribute to recall-like expansion/effector function of HMBPP-specific Vgamma2Vdelta2 T cells after Mycobacterium tuberculosis infection or vaccination.
Authors: Shen H, Wang Y, Chen C, Frencher J, Huang D, Yang E, Ryan-Payseur B, Chen Z
Eur J Immunol, 2015-02-01;45(2):442-51.
Species: Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Sample Types: Whole Cells
Applications: Bioassay, Neutralization
The human IL-23 receptor rs11209026 A allele promotes the expression of a soluble IL-23R-encoding mRNA species.
Authors: Yu R, Brazaitis J, Gallagher G
J Immunol, 2014-12-31;194(3):1062-8.
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)
Characterization of human CD39+ Th17 cells with suppressor activity and modulation in inflammatory bowel disease.
Authors: Longhi M, Moss A, Bai A, Wu Y, Huang H, Cheifetz A, Quintana F, Robson S
PLoS ONE, 2014-02-05;9(2):e87956.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Blocking of alpha4beta7 gut-homing integrin during acute infection leads to decreased plasma and gastrointestinal tissue viral loads in simian immunodeficiency virus-infected rhesus macaques.
Authors: Ansari AA, Reimann KA, Mayne AE, Takahashi Y, Stephenson ST, Wang R, Wang X, Li J, Price AA, Little DM, Zaidi M, Lyles R, Villinger F
J. Immunol., 2010-12-13;186(2):1044-59.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Flow Cytometry
A naturally occurring, soluble antagonist of human IL-23 inhibits the development and in vitro function of human Th17 cells.
Authors: Yu RY, Gallagher G
J. Immunol., 2010-11-12;185(12):7302-8.
Species: Human
Sample Types: Cell Culture Supernates, Recombinant Protein
Applications: ELISA Development, Western Blot
Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
Authors: Cabezon R, Sintes J, Llinas L, Benitez-Ribas D
Immunol. Lett., 2010-10-07;134(2):167-73.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Low expression of the IL-23/Th17 pathway in atopic dermatitis compared to psoriasis.
Authors: Guttman-Yassky E, Lowes MA, Fuentes-Duculan J, Zaba LC, Cardinale I, Nograles KE, Khatcherian A, Novitskaya I, Carucci JA, Bergman R, Krueger JG
J. Immunol., 2008-11-15;181(10):7420-7.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr