Human IL-33 Propeptide Antibody

Catalog # Availability Size / Price Qty
Detection of Human IL‑33 Propeptide by Western Blot.
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Product Details
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Human IL-33 Propeptide Antibody Summary

Species Reactivity
Detects human IL-33 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant human IL-33 and mature recombinant mouse IL-33 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human IL-33 Propeptide
Accession # O95760
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL‑33 Propeptide antibody by Western Blot. View Larger

Detection of Human IL‑33 Propeptide by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-33 Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL-33 Propeptide at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry IL-33 antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

IL‑33 in HUVEC Human Cells. IL-33 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human IL-33 Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4810) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-33

IL-33 (interleukin 33; also NF-HEV and IL-1F11) is a 31-32 kDa member of the IL-1 family of proteins. It is expressed in endothelial cells and reportedly has two functions; first, it induces Th2-type cytokines, and second, it acts as a nuclear transcription factor. Human IL-33 is 270 amino acids (aa) in length. It contains a putative proregion (aa 1-111) and a mature segment (aa 112-270). The proregion contains a bipartite NLS (aa 61-78), plus an  alpha -helical homeodomain-like HTH motif (aa 1-65). The HTH motif mediates nuclear localization and heterochromatin association. A potential caspase 1 cleavage site exists in human IL-33 (Ser111-Ser112), but not in IL-33 from canine, porcine or bovine. There is one splice variant that shows a deletion of aa 113-118. Over aa 1-111, human IL-33 propeptide is 45% aa identical to mouse IL-33 propeptide.

Long Name
Interleukin 33
Entrez Gene IDs
90865 (Human); 77125 (Mouse)
Alternate Names
C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2

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