Human IL-33 Propeptide Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-33 Propeptide in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with mature recombinant human IL-33 and mature recombinant mouse IL-33 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human IL-33 Propeptide
    Met1-Ser111
    Accession # O95760
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human IL‑33 Propeptide by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, Raji human Burkitt's lymphoma cell line, and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL‑33 Propeptide Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4810) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL‑33 Propeptide at approximately 31 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunocytochemistry
IL‑33 in HUVEC Human Cells.

IL‑33 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human IL‑33 Propeptide Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4810) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern­Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-33
IL-33 (interleukin 33; also NF-HEV and IL-1F11) is a 31-32 kDa member of the IL-1 family of proteins. It is expressed in endothelial cells and reportedly has two functions; first, it induces Th2-type cytokines, and second, it acts as a nuclear transcription factor. Human IL-33 is 270 amino acids (aa) in length. It contains a putative proregion (aa 1-111) and a mature segment (aa 112-270). The proregion contains a bipartite NLS (aa 61-78), plus an  alpha -helical homeodomain-like HTH motif (aa 1-65). The HTH motif mediates nuclear localization and heterochromatin association. A potential caspase 1 cleavage site exists in human IL-33 (Ser111-Ser112), but not in IL-33 from canine, porcine or bovine. There is one splice variant that shows a deletion of aa 113-118. Over aa 1-111, human IL-33 propeptide is 45% aa identical to mouse IL-33 propeptide.
  • Long Name:
    Interleukin 33
  • Entrez Gene IDs:
    90865 (Human); 77125 (Mouse)
  • Alternate Names:
    C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2
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