Human interleukin 1 family member #9 [IL-1F9; also named Interleukin-36 gamma, IL36G, IL-1 epsilon (epsilon) and IL-1H1] is a member of the IL-1 family, which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (1‑4). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F9 is synthesized as a 19 kDa, 169 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site (1, 2, 5). The molecule is secreted when transfected into 293-T cells (5). Human to mouse, IL-1F9 has 53% aa sequence identity. Within the family, IL-1F9 shares 30%, 23%, 33%, 57%, 35%, 45%, and 32% aa sequence identity with IL-1 ra, IL-1 beta, IL-1F5, F6, F7, F8, and F10, respectively. Cells reported to express IL-1F9 include Langerhans cells, keratinocytes/stratified squamous epithelium, plus Chief cells and Parietal cells of the stomach (4, 5). The receptor for IL‑1F9 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL-1F9, along with IL-1F8 and IL-1F6, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.
Human IL‑36 gamma /IL‑1F9 Antibody
R&D Systems | Catalog # AF2320
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Blockade of Receptor-ligand Interaction
Cited:
Immunohistochemistry, Western Blot, ELISA Development
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
E. coli-derived recombinant human IL‑36 gamma /IL‑1F9 (R&D Systems, Catalog # 2320-IL)
Met1-Asp169
Accession # Q9NZH8
Met1-Asp169
Accession # Q9NZH8
Specificity
Detects human IL‑36 gamma /IL‑1F9 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) IL-36 alpha is observed, approximately 5% cross-reactivity with rhIL-37 is observed, and less than 1% cross-reactivity with rhIL-1 alpha, rhIL-1 beta, rhIL‑36Ra, and rhIL‑36 beta is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IL‑36 gamma /IL‑1F9 Antibody
Detection of Human IL‑36 gamma /IL‑1F9 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 6 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-36 gamma/IL-1F9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-36 gamma/IL-1F9 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human IL‑36 gamma /IL‑1F9 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.7-2.8 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera (Catalog # 872-RP) to immobilized Recombinant Human IL‑36 gamma /IL‑1F9 (Catalog # 2320-IL) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
Western Blot
0.5 µg/mL
Sample: A431 human epithelial carcinoma cell line treated with Recombinant Human IFN‑ gamma (Catalog # 285-IF) and Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Sample: A431 human epithelial carcinoma cell line treated with Recombinant Human IFN‑ gamma (Catalog # 285-IF) and Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Reviewed Applications
Read 1 review rated 5 using AF2320 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IL-36 gamma/IL-1F9
References
- Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
- Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
- Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
- Dunn, E. et al. (2001) Trends Immunol. 22:533.
- Debets, R. et al. (2001) J. Immunol. 167:1440.
- Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 36 gamma/Interleukin 1 Family 9
Alternate Names
IL-1 H1, IL-1rp2, IL1F9, IL36 gamma, IL36G
Gene Symbol
IL36G
UniProt
Additional IL-36 gamma/IL-1F9 Products
Product Documents for Human IL‑36 gamma /IL‑1F9 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human IL‑36 gamma /IL‑1F9 Antibody
For research use only
Related Research Areas
Citations for Human IL‑36 gamma /IL‑1F9 Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
