Human IL-36 gamma /IL-1F9 Antibody

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Detection of Human IL‑36 gamma /IL‑1F9 by Western Blot.
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Product Details
Citations (6)
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Reviews (1)

Human IL-36 gamma /IL-1F9 Antibody Summary

Species Reactivity
Detects human IL‑36 gamma /IL‑1F9 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human (rh) IL-36 alpha is observed, approximately 5% cross-reactivity with rhIL-37 is observed, and less than 1% cross-reactivity with rhIL-1 alpha, rhIL-1 beta, rhIL‑36Ra, and rhIL‑36 beta is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human IL‑36 gamma /IL‑1F9 (R&D Systems, Catalog # 2320-IL)
Accession # Q9NZH8
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.7-2.8 µg/mL of this antibody will block 50% of the binding of 5 μg/mL of Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera (Catalog # 872-RP) to immobilized Recombinant Human IL‑36 gamma /IL‑1F9 (Catalog # 2320-IL) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IL-36?/IL-1F9 antibody by Western Blot. View Larger

Detection of Human IL‑36 gamma /IL‑1F9 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 6 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-36 gamma/IL-1F9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-36 gamma/IL-1F9 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 gamma/IL-1F9

Human interleukin 1 family member #9 [IL-1F9; also named Interleukin-36 gamma, IL36G, IL-1 epsilon  (epsilon) and IL-1H1] is a member of the IL-1 family, which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (1‑4). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F9 is synthesized as a 19 kDa, 169 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site (1, 2, 5). The molecule is secreted when transfected into 293-T cells (5). Human to mouse, IL-1F9 has 53% aa sequence identity. Within the family, IL-1F9 shares 30%, 23%, 33%, 57%, 35%, 45%, and 32% aa sequence identity with IL-1 ra, IL-1 beta, IL-1F5, F6, F7, F8, and F10, respectively. Cells reported to express IL-1F9 include Langerhans cells, keratinocytes/stratified squamous epithelium, plus Chief cells and Parietal cells of the stomach (4, 5). The receptor for IL‑1F9 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL-1F9, along with IL-1F8 and IL-1F6, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.

  1. Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  4. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  5. Debets, R. et al. (2001) J. Immunol. 167:1440.
  6. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Long Name
Interleukin 36 gamma/Interleukin 1 Family 9
Entrez Gene IDs
56300 (Human); 215257 (Mouse); 499744 (Rat)
Alternate Names
IL-1 epsilon; IL-1 H1; IL-1 Related Protein 2; IL-1(EPSILON); IL1E; IL-1-epsilon; IL1F9; IL-1F9; IL1H1; IL-1H1; IL-1-Related Protein 2; IL1RP2; IL-1rp2; IL36 gamma; IL-36 gamma; IL36G; interleukin 1 family, member 9; interleukin 1-related protein 2; Interleukin 36, Gamma; Interleukin-1 epsilon; interleukin-1 family member 9; Interleukin-1 homolog 1; Interleukin-36 Gamma

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Citations for Human IL-36 gamma /IL-1F9 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. IL-36 receptor agonist and antagonist imbalance drives neutrophilic inflammation in COPD
    Authors: Jonathan R. Baker, Peter S. Fenwick, Carolin K. Koss, Harriet B. Owles, Sarah L. Elkin, Jay Fine et al.
    JCI Insight
  2. Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells
    Authors: JM Murrieta-C, RN Gutiérrez-, HM El-Shorafa, T Groten, S Rodríguez-, ME Cancino-Di, JC Cancino-Di, RR Favaro, UR Markert, DM Morales-Pr
    International Journal of Molecular Sciences, 2020-12-30;22(1):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. IL-36 gamma drives skin toxicity induced by EGFR/MEK inhibition and commensal Cutibacterium acnes
    Authors: Takashi K. Satoh, Mark Mellett, Barbara Meier-Schiesser, Gabriele Fenini, Atsushi Otsuka, Hans-Dietmar Beer et al.
    Journal of Clinical Investigation
  4. Application of IL-36 receptor antagonist weakens CCL20 expression and impairs recovery in the late phase of murine acetaminophen-induced liver injury.
    Authors: Scheiermann P, Bachmann M, Hardle L, Pleli T, Piiper A, Zwissler B, Pfeilschifter J, Muhl H
    Sci Rep, 2015-02-17;5(0):8521.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. IL-36gamma/IL-1F9, an innate T-bet target in myeloid cells.
    Authors: Bachmann M, Scheiermann P, Hardle L, Pfeilschifter J, Muhl H
    J Biol Chem, 2012-10-24;287(50):41684-96.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  6. The double-stranded RNA analogue polyinosinic-polycytidylic acid induces keratinocyte pyroptosis and release of IL-36 gamma.
    Authors: Lian LH, Milora KA, Manupipatpong KK, Jensen LE
    J. Invest. Dermatol., 2012-02-09;132(5):1346-53.
    Species: Human
    Sample Types: Cell Culture Supernates, Cell Lysates
    Applications: ELISA Development, Western Blot


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Human IL-36 gamma /IL-1F9 Antibody
By Anonymous on 08/17/2020
Application: Func Sample Tested: Peripheral blood cells Species: Human