Human IL-36 gamma /IL-1F9 Antibody Summary
Accession # Q9NZH8
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human IL‑36 gamma /IL‑1F9 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human IFN‑ gamma (Catalog # 285‑IF) and 20 ng/mL Recombinant Human TNF‑ alpha (Catalog # 210‑TA) for 6 hours. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL‑36 gamma /IL‑1F9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2320) followed by HRP‑conjugated Anti‑Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑36 gamma /IL‑1F9 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-36 gamma/IL-1F9
Human interleukin 1 family member #9 [IL-1F9; also named Interleukin-36 gamma, IL36G, IL-1 epsilon (epsilon) and IL-1H1] is a member of the IL-1 family, which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (1‑4). All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). IL-1F9 is synthesized as a 19 kDa, 169 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site (1, 2, 5). The molecule is secreted when transfected into 293-T cells (5). Human to mouse, IL-1F9 has 53% aa sequence identity. Within the family, IL-1F9 shares 30%, 23%, 33%, 57%, 35%, 45%, and 32% aa sequence identity with IL-1 ra, IL-1 beta, IL-1F5, F6, F7, F8, and F10, respectively. Cells reported to express IL-1F9 include Langerhans cells, keratinocytes/stratified squamous epithelium, plus Chief cells and Parietal cells of the stomach (4, 5). The receptor for IL‑1F9 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (6). Recombinant IL-1F9, along with IL-1F8 and IL-1F6, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1 Rrp2 dependent manner.
- Smith, D.E. et al. (2000) J. Biol. Chem. 275:1169.
- Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
- Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
- Dunn, E. et al. (2001) Trends Immunol. 22:533.
- Debets, R. et al. (2001) J. Immunol. 167:1440.
- Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.
Citations for Human IL-36 gamma /IL-1F9 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Application of IL-36 receptor antagonist weakens CCL20 expression and impairs recovery in the late phase of murine acetaminophen-induced liver injury.
Authors: Scheiermann P, Bachmann M, Hardle L, Pleli T, Piiper A, Zwissler B, Pfeilschifter J, Muhl H
Sci Rep, 2015;5(0):8521.
Sample Types: Cell Lysates
The double-stranded RNA analogue polyinosinic-polycytidylic acid induces keratinocyte pyroptosis and release of IL-36 gamma.
Authors: Lian LH, Milora KA, Manupipatpong KK, Jensen LE
J. Invest. Dermatol., 2012;132(5):1346-53.
Sample Types: Cell Culture Supernates
Applications: ELISA Development
IL-36gamma/IL-1F9, an innate T-bet target in myeloid cells.
Authors: Bachmann M, Scheiermann P, Hardle L, Pfeilschifter J, Muhl H
J Biol Chem, 2012;287(50):41684-96.
Sample Types: Cell Culture Supernates
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