Human IL-38/IL-1F10 Antibody
Human IL-38/IL-1F10 Antibody Summary
Accession # Q8WWZ1
This antibody functions as an ELISA capture antibody when paired with Goat Anti-Human IL‑38/IL‑1F10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2427).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human IL-38/IL-1F10 DuoSet ELISA Kit (Catalog # DY9110-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Human IL‑38/IL‑1F10 ELISA Standard Curve. Recombinant Human IL-38/IL-1F10 protein was serially diluted 2-fold and captured by Mouse Anti-Human IL-38/IL-1F10 Monoclonal Antibody (Catalog # MAB24271) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL-38/IL-1F10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2427) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human IL-38, previously called IL-1F10 or IL-1HY2, is a secreted cytokine belonging to the IL-1 family. It is synthesized as a 152 amino acid mature protein that lacks an identifiable signal sequence. It binds to soluble IL-1 RI. Human IL-38/IL-1F10 shares 82% aa sequence identity with mouse IL-38/IL-1F10. Human IL-38/IL-1F10 also shares 39% and 27% aa sequence identity with IL-1ra/IL-1F3 and IL-1 beta /IL-1F2, respectively.
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