Human Importin alpha 2/KPNA2 Antibody (682239)

R&D Systems | Catalog # MAB6207

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 682239
View all Importin alpha 2/KPNA2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Importin  alpha 2/KPNA2
Ser2-Asp132
Accession # P52292

Specificity

Detects human Importin  alpha 2/KPNA2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human KPNA1, A3, A4, A5, or B1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Importin alpha 2/KPNA2 Antibody (682239)

Detection of Human Importin a2/KPNA2 antibody by Western Blot.

Detection of Human Importin alpha 2/KPNA2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Importin a2/KPNA2 Monoclonal Antibody (Catalog # MAB6207) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Importin a2/KPNA2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Importin a2/KPNA2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Importin alpha 2/KPNA2 in HeLa Human Cell Line.

Importin a2/KPNA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Importin a2/KPNA2 Monoclonal Antibody (Catalog # MAB6207) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Importin alpha 2/KPNA2 by Western Blot

Detection of Importin alpha 2/KPNA2 by Western Blot

GM130 K34A mutation causes proliferative defects and G2/M arrest. a GST pulldown assays of Importin-alpha FL with GM130-S25D mutants. GST-fused GM130-S25D mutants were incubated with recombinant Importin-alpha FL. GST-bound samples were analyzed by SDS–PAGE and stained with Coomassie blue. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31541088), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Importin alpha 2/KPNA2 by Western Blot

Detection of Importin alpha 2/KPNA2 by Western Blot

GM130 interacts with full-length Importin-alpha. a, b ITC titration curves (upper) and binding isotherms (lower) of Importin-alpha FL with GM130-NLS WT (a) and GM130-NLS phospho-S25 (b). Kd values are indicated. c Pulldown assays of Importin-alpha FL with GM130-WT or GM130-S25D. GST-tagged GM-130-WT or GM130-S25D was incubated with Importin-alpha FL. GST-bound samples were analyzed by SDS–PAGE and stained with Coomassie blue. d Competition binding assay. p115-CTD was incubated with either GST-GM130-WT or GST-GM130-S25D in the presence of indicated concentrations of Importin-alpha FL. Samples pulled down by GST beads were analyzed by SDS–PAGE and stained with Coomassie blue. e, f Band intensities of Importin-alpha FL and p115-CTD from the SDS–PAGE gels in d were quantified and normalized by GST-GM130 intensity. Quantitative plots show normalized Importin-alpha FL intensities (e) and percent p115-CTD remaining on GM130 (f). Data represent mean ± standard deviation from three independent experiments Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31541088), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Importin alpha 2/KPNA2 Antibody (682239)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Importin alpha 2/KPNA2

Importin alpha 2, also known as KPNA2 (Karyopherin subunit alpha 2), SRP1a, or RAG1, is a 58-60 kDa member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. Importin alpha 2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, Importin alpha 2 first binds to NBS1, and then to Importin beta 1, which mediates movement through the nuclear pore. Human Importin alpha 2 is 529 amino acids (aa) in length. It contains an N-terminal IBB/Importin beta domain (aa 2-60), ten Armadillo repeats that bind “cargo” (aa 71-496) and two intervening NLS binding sites. There is one splice variant that shows a deletion of aa 72-310, coupled to an 11 aa substitution for aa 389-406. Over aa 1-132, human Importin alpha 2 shares 94% aa identity with mouse Importin alpha 2.

Long Name

Karyopherin alpha 2

Alternate Names

Importin alpha 1, IPOA1, KPNA2, Pendulin, QIP2, RCH1, SRP1-alpha

Entrez Gene IDs

3838 (Human); 16647 (Mouse); 85245 (Rat)

Gene Symbol

KPNA2

UniProt

P52292 (Human)

Additional Importin alpha 2/KPNA2 Products

Product Documents for Human Importin alpha 2/KPNA2 Antibody (682239)

Certificate of Analysis

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Product Specific Notices for Human Importin alpha 2/KPNA2 Antibody (682239)

For research use only

Related Research Areas

Cancer Biomarkers
Cell Cycle

Citations for Human Importin alpha 2/KPNA2 Antibody (682239)

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Protocols

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