Human Importin  alpha 2/KPNA2 Antibody

Catalog # Availability Size / Price Qty
MAB6207
MAB6207-SP
Detection of Human Importin  alpha 2/KPNA2 by Western Blot.
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Product Details
Citations (4)
FAQs
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Human Importin  alpha 2/KPNA2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Importin  alpha 2/KPNA2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human KPNA1, A3, A4, A5, or B1 is observed.
Source
Monoclonal Mouse IgG2A Clone # 682239
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Importin  alpha 2/KPNA2
Ser2-Asp132
Accession # P52292
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Importin a2/KPNA2 antibody by Western Blot. View Larger

Detection of Human Importin alpha 2/KPNA2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human Importin a2/KPNA2 Monoclonal Antibody (Catalog # MAB6207) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Importin a2/KPNA2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Importin a2/KPNA2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Importin alpha 2/KPNA2 in HeLa Human Cell Line. Importin a2/KPNA2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Importin a2/KPNA2 Monoclonal Antibody (Catalog # MAB6207) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Importin alpha 2/KPNA2

Importin alpha 2, also known as KPNA2 (Karyopherin subunit alpha 2), SRP1a, or RAG1, is a 58-60 kDa member of the Importin alpha family of proteins. It is ubiquitously expressed, and found in both nucleus and cytoplasm. Importin alpha 2 functions as a cargo carrier that transports various complexes from cytoplasm into nucleus. For the MRN DNA repair complex, Importin alpha 2 first binds to NBS1, and then to Importin beta 1, which mediates movement through the nuclear pore. Human Importin alpha 2 is 529 amino acids (aa) in length. It contains an N-terminal IBB/Importin beta domain (aa 2-60), ten Armadillo repeats that bind “cargo” (aa 71-496) and two intervening NLS binding sites. There is one splice variant that shows a deletion of aa 72-310, coupled to an 11 aa substitution for aa 389-406. Over aa 1-132, human Importin alpha 2 shares 94% aa identity with mouse Importin alpha 2.

Long Name
Karyopherin alpha 2
Entrez Gene IDs
3838 (Human); 16647 (Mouse); 85245 (Rat)
Alternate Names
Importin alpha 1; Importin alpha 2; importin-alpha-P1; IPOA1; karyopherin alpha 2 (RAG cohort 1, importin alpha 1); Karyopherin subunit alpha-2; KPNA2; Pendulin; QIP2; QIP2importin alpha 2; RAG cohort 1; RAG cohort protein 1; RCH1; RCH1importin subunit alpha-2; SRP1; SRP1alpha; SRP1-alpha

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Citations for Human Importin  alpha 2/KPNA2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Mechanical force application to the nucleus regulates nucleocytoplasmic transport
    Authors: I Andreu, I Granero-Mo, NR Chahare, K Clein, M Molina-Jor, AEM Beedle, A Elosegui-A, JF Abenza, L Rossetti, X Trepat, B Raveh, P Roca-Cusac
    Nature Cell Biology, 2022-06-09;24(6):896-905.
  2. Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death
    Authors: Gasset-Rosa F, Lu S, Yu H et al.
    Neuron
  3. Ran pathway-independent regulation of mitotic Golgi disassembly by Importin-α
    Authors: CC Chang, CJ Chen, C Grauffel, YC Pien, C Lim, SY Tsai, KC Hsia
    Nat Commun, 2019-09-20;10(1):4307.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Immunoprecipitation
  4. Ciliary Entry of the Hedgehog Transcriptional Activator Gli2 Is Mediated by the Nuclear Import Machinery but Differs from Nuclear Transport in Being Imp-?/?1-Independent
    PLoS ONE, 2016-08-31;11(8):e0162033.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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