Human INHBE Antibody

R&D Systems | Catalog # MAB11746

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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1111910
View all INHBE Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide
Accession # P58166

Specificity

Detects a sythetic peptide specific for human IHNBE around amino acid 200 in Direct ELISA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human INHBE Antibody

Detection of INHBE in Human Liver.

INHBE was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human INHBE Monoclonal Antibody (Catalog # MAB11746) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human INHBE Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: INHBE

Inhibin beta E (INHBE) is a member of the transforming growth factor-beta (TGF-beta ) superfamily of cytokines, with a molecular weight of approximately 40 kDa. INHBE plays critical roles in the regulation of diverse physiological processes, including cell growth, differentiation, and metabolism. It is a key component of the activin signaling pathway, where it dimerizes with other beta -subunits to form bioactive ligands involved in endocrine regulation. INHBE is primarily expressed in hepatic and reproductive tissues and has been implicated in metabolic homeostasis, particularly in energy expenditure and glucose metabolism. Dysregulation of INHBE expression has been linked to pathophysiological conditions such as obesity, insulin resistance, and liver disease. Furthermore, INHBE has gained attention for its tumor-suppressive role in certain cancers, suggesting its potential as a regulator of cell proliferation and apoptosis. Its specificity and involvement in metabolic and oncogenic pathways highlight INHBE as a promising biomarker and a potential target for therapeutic strategies.

References

  1. Hedger MP, de Kretser DM. The activins and their binding protein, follistatin-Diagnostic and therapeutic targets in inflammatory disease and fibrosis. Cytokine Growth Factor Rev. 2013 Jun;24(3):285-95. doi: 10.1016/j.cytogfr.2013.03.003. Epub 2013 Mar 29. PMID: 23541927.
  2. Hashimoto O, Funaba M, Sekiyama K, Doi S, Shindo D, Satoh R, Itoi H, Oiwa H, Morita M, Suzuki C, Sugiyama M, Yamakawa N, Takada H, Matsumura S, Inoue K, Oyadomari S, Sugino H, Kurisaki A. Activin E Controls Energy Homeostasis in Both Brown and White Adipose Tissues as a Hepatokine. Cell Rep. 2018 Oct 30;25(5):1193-1203. doi: 10.1016/j.celrep.2018.10.008. PMID: 30380411.
  3. Griffin JD, Buxton JM, Culver JA, Barnes R, Jordan EA, White AR, Flaherty SE, Bernardo B, Ross T, Bence KK, Birnbaum MJ. Hepatic Activin E mediates liver-adipose inter-organ communication, suppressing adipose lipolysis in response to elevated serum fatty acids. Mol Metab. 2023 Dec;78:101830. doi: 10.1016/j.molmet.2023.101830. Epub 2023 Oct 28. PMID: 38787338; PMCID: PMC10656223.

Long Name

Inhibin Subunit Beta E

Alternate Names

Inhibin Beta E, MGC4638

Entrez Gene IDs

83729 (Human); 16326 (Mouse)

Gene Symbol

INHBE

UniProt

P58166 (Human)

Additional INHBE Products

Product Documents for Human INHBE Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human INHBE Antibody

For research use only

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Protocols

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