Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all IRAK4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IRAK4
Met1-Ser460
Accession # Q9NWZ3

Specificity

Detects human IRAK4 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) IRAK1 and rhIRAK2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IRAK4 Antibody

Detection of Human IRAK4 antibody by Western Blot.

Detection of Human IRAK4 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human IRAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3919) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). For additional reference, recombinant human IRAK1, IRAK2, and IRAK4 (2 ng/lane) were included. A specific band for IRAK4 was detected at approxi­mately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IRAK4 antibody in THP-1 Human Cell Line by Immunocytochemistry (ICC).

IRAK4 in THP‑1 Human Cell Line.

IRAK4 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Goat Anti-Human IRAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3919) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Human IRAK4 by Simple WesternTM.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and THP‑1 human acute monocytic leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for IRAK4 at approximately 55 kDa (as indicated) using 20 µg/mL of Goat Anti-Human IRAK4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3919). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human IRAK4 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human peripheral blood mononuclear cells and immersion fixed THP‑1 human acute monocytic leukemia cell line

Simple Western

20 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and THP‑1 human acute monocytic leukemia cell line

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IRAK4

IL-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that help mediate signaling from Toll-like receptor (TLR) and IL-1 receptor family members. Four human IRAKs have been identified: IRAK1, IRAK2, IRAK-M, and IRAK4. Upon TLR ligand challenge, IRAK4 knockout mice exhibit severely impaired NF-kappa B activation. IRAK4 mutations have been described in patients with recurrent bacterial infections and poor inflammatory responses.

Long Name

Interleukin-1 Receptor-Associated Kinase 4

Alternate Names

REN64

Entrez Gene IDs

51135 (Human); 266632 (Mouse); 300177 (Rat)

Gene Symbol

IRAK4

UniProt

Q9NWZ3

Additional IRAK4 Products

Product Documents for Human IRAK4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human IRAK4 Antibody

For research use only

Citations for Human IRAK4 Antibody

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Protocols

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