Human IRF4 Antibody

Catalog # Availability Size / Price Qty
MAB55251-100
MAB55251-SP

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Detection of Human IRF4 by Western Blot.
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Human IRF4 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IRF4 in direct ELISAs.
Source
Monoclonal Mouse IgG2B Clone # 503210
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IRF4
Glu130-Glu451
Accession # Q15306
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Ramos human Burkitt's lymphoma cell line and HDLM‑2 human Hodgkin's lymphoma cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human IRF4 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, HDLM‑2 human Hodgkin’s lymphoma cell line, U937 human histiocytic lymphoma cell line (negative control), and HUVEC human umbilical vein endothelial cells (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IRF4 Monoclonal Antibody (Catalog # MAB55251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for IRF4 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IRF4

Interferon Regulatory Factor 4 (IRF4), also known as MUM1 and LSIRF, is a 51 kDa lymphocyte-restricted transcription factor. It is required for immunoglobulin class switching and terminal differentiation of B cells. IRF4 is overexpressed in multiple myeloma and cooperates with Myc in an autoregulatory loop. In T cells, IRF4 is required for the production of IL-4. IRF4 contains an N-terminal DNA binding domain that is homologous to that in other IRF proteins. Within the C-terminal domain (aa 130‑451), human IRF4 shares 90% aa sequence identity with mouse and rat IRF4. Alternate splicing may generate isoforms with N-terminal, C-terminal, or internal deletions.

Long Name
Interferon Regulatory Factor 4
Entrez Gene IDs
3662 (Human); 16364 (Mouse); 291100 (Rat)
Alternate Names
interferon regulatory factor 4; IRF4; IRF-4; LSIRF; LSIRFMUM1; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM1; NF-EM5

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Human IRF4 Antibody
By Anonymous on 02/05/2022
Application: WB Sample Tested: HDLM‑2 human Hodgkin’s lymphoma cell line Species: Human