Human Jade-1/PHF17 Antibody

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Detection of Human Jade‑1/PHF17 by Western Blot.
2 Images
Product Details
Supplemental Products

Human Jade-1/PHF17 Antibody Summary

Species Reactivity
Detects human JADE-1/PHF-17 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant human Jade-2/PHF15 is observed.
Monoclonal Mouse IgG2B Clone # 658005
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human JADE-1/PHF-17
Accession # Q6IE81
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Jade-1/PHF17 antibody by Western Blot. View Larger

Detection of Human Jade‑1/PHF17 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Jade‑1/PHF17 Monoclonal Antibody (Catalog # MAB6275) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Jade‑1/PHF17 at approximately 92 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Jade-1/PHF17 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

Jade‑1/PHF17 in HeLa Human Cell Line. Jade-1/PHF17 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Jade-1/PHF17 Monoclonal Antibody (Catalog # MAB6275) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Jade-1/PHF17

Jade-1 (Gene for Apoptosis and Differentiation in Epithelia; also PHF17) is a 94 kDa member of the JADE family of proteins. It is expressed in renal tubule cells as well as embryonic primitive streak (in mouse). Initially, Jade-1 was described as a proapoptotic tumor suppressor. Jade-1 also interacts with the HBO1 complex to specifically acetylate nucleosome histones, thus acting as a transcriptional activator that likely impacts DNA replication, recombination and repair. Human Jade-1 is 842 amino acids (aa) in length. It contains one PEST domain with multiple Ser phosphorylation sites (aa 5-28), and two PHD Zinc fingers (aa 203-253 and 312-371) that are required for histone acetylation. There are two splice variants, one that is 61-64 kDa in size and contains a 7 aa substitution for aa 503-842, and another that shows a deletion of aa 99-110. Over aa 1-137, human Jade-1 shares 96% aa identity with mouse Jade-1.

Entrez Gene IDs
79960 (Human); 269424 (Mouse); 310352 (Rat)
Alternate Names
FLJ22479; Jade1; Jade-1; KIAA1807; PHD finger protein 17JADE1gene for apoptosis and differentiation in epithelia; PHD protein Jade-1; PHF17; protein Jade-1

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Jade-1/PHF17 Antibody

There are currently no reviews for this product. Be the first to review Human Jade-1/PHF17 Antibody and earn rewards!

Have you used Human Jade-1/PHF17 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review