Human Jagged 2 Antibody

(2 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Jagged 2 in direct ELISAs and Western blots. In Western blots, approximately 35% cross-reactivity with recombinant human Jagged 1 is observed and less than 1% cross-reactivity with recombinant rat Jagged 1 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Jagged 2
    Met27-Asp307
    Accession # Q9Y219
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Jagged 2 Fc Chimera (Catalog # 1726-JG)
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed paraffin-embedded sections of human skeletal muscle
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Jagged 2 in HUVEC Human Cells. Jagged 2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Jagged 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Jagged 2

Human Jagged 2 is a 131 kDa (predicted) member of the Delta-Serrate-Lag-2 (DSL) family of ligands. This family activates LIN12/Notch proteins and thereby regulates cell fate determination during development (1-5). It is a type 1 transmembrane protein that is synthesized as a 1238 amino acid (aa) precursor. It contains a 23 aa signal sequence, a large 1057 aa extracellular region, a 21 aa transmembrane region, and a short 137 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), a von Willebrand factor (vWF) type C domain, and a cysteine-rich region just proximal to the transmembrane segment (2). There are two isoforms for human Jagged 2, named long and short. The short form lacks a splicing variant region (aa 421-461) that is present in the long form of the protein. Human Jagged 2 shares 90% and 87% aa sequence identity with mouse and rat Jagged 2, respectively. During murine embryonic development, Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (2). In 2 week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (2). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (2). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, T cell lineage differentiation, natural killer cells, and the establishment of functional natural killer cell lines (3, 5, 6).

  • References:
    1. Shawber, C. et al. (1996) Dev. Biol. 180:370.
    2. Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
    3. Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
    4. Schickwann, T. et al. (2000) Blood 96:950.
    5. DeHart, S. et al. (2005) Blood 105:3521.
    6. de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.
  • Entrez Gene IDs:
    3714 (Human); 16450 (Mouse); 29147 (Rat)
  • Alternate Names:
    HJ2; JAG2; Jagged 2; Jagged2; protein jagged-2; SER2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Hypoxia-induced Jagged2 promotes breast cancer metastasis and self-renewal of cancer stem-like cells.
    Authors: Xing F, Okuda H, Watabe M, Kobayashi A, Pai SK, Liu W, Pandey PR, Fukuda K, Hirota S, Sugai T, Wakabayshi G, Koeda K, Kashiwaba M, Suzuki K, Chiba T, Endo M, Mo YY, Watabe K
    Oncogene, 2011;30(39):4075-86.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Differential induction of Th1-prone immunity by human dendritic cells activated with Sporothrix schenckii of cutaneous and visceral origins to determine their different virulence.
    Authors: Uenotsuchi T, Takeuchi S, Matsuda T, Urabe K, Koga T, Uchi H, Fukagawa S, Kawasaki M, Kajiwara H, Moroi Y, Furue M
    Int. Immunol., 2006;18(12):1637-46.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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