Detects human Jagged 2 in direct ELISAs and Western blots. In Western blots, approximately 35% cross-reactivity with recombinant human Jagged 1 is observed and less than 1% cross-reactivity with recombinant rat Jagged 1 is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant human Jagged 2 Met27-Asp307 Accession # Q9Y219
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Jagged 2 in HUVEC Human Cells.
Jagged 2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Jagged 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Jagged 2
Human Jagged 2 is a 131 kDa (predicted) member of the Delta-Serrate-Lag-2 (DSL) family of ligands. This family activates LIN12/Notch proteins and thereby regulates cell fate determination during development (1-5). It is a type 1 transmembrane protein that is synthesized as a 1238 amino acid (aa) precursor. It contains a 23 aa signal sequence, a large 1057 aa extracellular region, a 21 aa transmembrane region, and a short 137 aa cytoplasmic region. The extracellular region contains four potential N-linked glycosylation sites, a DSL domain, 16 EGF-like repeats (many of which are also sites of calcium binding), a von Willebrand factor (vWF) type C domain, and a cysteine-rich region just proximal to the transmembrane segment (2). There are two isoforms for human Jagged 2, named long and short. The short form lacks a splicing variant region (aa 421-461) that is present in the long form of the protein. Human Jagged 2 shares 90% and 87% aa sequence identity with mouse and rat Jagged 2, respectively. During murine embryonic development, Jagged 2 is expressed highest in fetal thymus, epidermis, foregut, dorsal root ganglia, and inner ear (2). In 2 week old mice, the Jagged 2 transcript is most abundant in heart, lung, thymus, skeletal muscle, brain, and testis (2). Functionally, it is suggested that Jagged 2 engages the Notch1 pathway of signal transduction (2). It is involved in the development of the mammalian limb, branchial arches, central and peripheral nervous systems, T cell lineage differentiation, natural killer cells, and the establishment of functional natural killer cell lines (3, 5, 6).
Shawber, C. et al. (1996) Dev. Biol. 180:370.
Luo, B. et al. (1997) Mol. Cell. Biol. 17:6057.
Valsecchi, V. et al. (1997) Mech. Dev. 69:203.
Schickwann, T. et al. (2000) Blood 96:950.
DeHart, S. et al. (2005) Blood 105:3521.
de La Coste, A. and A.A. Freitas (2006) Immunol. Lett. 102:1.
Entrez Gene IDs:
3714 (Human); 16450 (Mouse); 29147 (Rat)
HJ2; JAG2; Jagged 2; Jagged2; protein jagged-2; SER2
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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunocytochemistry/Immunofluorescence.