Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 674702
View all Jumonji/JARID2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Jumonji/JARID2
Met1-Thr159
Accession # Q92833

Specificity

Detects human Jumonji/JARID2 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Jumonji/JARID2 Antibody

Jumonji/JARID2 antibody in LNCaP Human Cell Line by Immunocytochemistry (ICC).

Jumonji/JARID2 in LNCaP Human Cell Line.

Jumonji/ JARID2 was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human Jumonji/JARID2 Monoclonal Antibody (Catalog # MAB6090) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Jumonji/JARID2 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed LNCaP human prostate cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Jumonji/JARID2

JARID2 (Jumonji/ARID domain-containing protein 2; also Jumonji [Japanese for “cruciform/cross”] and JMJ) is a 150-160 kDa member of the JMJ family of proteins. It is a nuclear complex coordinator that interacts with the G9a and GLP methyltransferases to repress gene transcription. JARID2 is expressed in mammalian embryonic neurons, megakaryocytes, hepatocytes and cardiomyocytes. Human JARID2 is 1246 amino acids (aa) in length. It contains an NLS (aa 1-131), a transcriptional repressor domain (aa 132-222), a DNA binding region (aa 558-709) and a nonfunctional histone demethylase domain (aa 916-1042). There are potential splice variants. One shows an alternative start site at Met173, another shows a deletion of aa 304-1151, and a third contains a 22 aa substitution for aa 1-60, coupled to a 50 aa substitution for aa 949-1246. Over aa 1-159, human JARID2 shares 96% aa identity with mouse JARID2.

Alternate Names

JARID2, JMJ

Entrez Gene IDs

3720 (Human); 16468 (Mouse)

Gene Symbol

JARID2

UniProt

Q92833 (Human)

Additional Jumonji/JARID2 Products

Product Documents for Human Jumonji/JARID2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Jumonji/JARID2 Antibody

For research use only

Related Research Areas

Epigenetics

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