Jumonji/JARID2 in LNCaP Human Cell Line.
Jumonji/ JARID2 was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human Jumonji/JARID2 Monoclonal Antibody (Catalog # MAB6090) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
JARID2 (Jumonji/ARID domain-containing protein 2; also Jumonji [Japanese for “cruciform/cross”] and JMJ) is a 150-160 kDa member of the JMJ family of proteins. It is a nuclear complex coordinator that interacts with the G9a and GLP methyltransferases to repress gene transcription. JARID2 is expressed in mammalian embryonic neurons, megakaryocytes, hepatocytes and cardiomyocytes. Human JARID2 is 1246 amino acids (aa) in length. It contains an NLS (aa 1-131), a transcriptional repressor domain (aa 132-222), a DNA binding region (aa 558-709) and a nonfunctional histone demethylase domain (aa 916-1042). There are potential splice variants. One shows an alternative start site at Met173, another shows a deletion of aa 304-1151, and a third contains a 22 aa substitution for aa 1-60, coupled to a 50 aa substitution for aa 949-1246. Over aa 1-159, human JARID2 shares 96% aa identity with mouse JARID2.
Entrez Gene IDs:
3720 (Human); 16468 (Mouse)
JARID2; JMJ; JMJjumonji-like protein; jumonji (mouse) homolog; jumonji homolog; Jumonji; jumonji, AT rich interactive domain 2; Jumonji/ARID domain-containing protein 2; protein Jumonji
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