Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2388C
View all KLRG1 Primary Antibodies »

Product Specifications

Immunogen

 Chinese hamster ovary cell line CHO-derived recombinant  human KLRG1
Leu60-Phe195
Accession # Q96E93

Specificity

Detects human KLRG1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human KLRG1 Antibody

Detection of KLRG1 antibody in Human Peripheral Blood antibody by Flow Cytometry.

Detection of KLRG1 in Human Peripheral Blood by Flow Cytometry.

Human peripheral blood was stained with (A) Rabbit anti-Human KLRG1 Monoclonal Antibody (Catalog # MAB70293) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111) and CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.
KLRG1 antibody in Human Tonsil by Immunohistochemistry (IHC-P).

KLRG1 in Human Tonsil.

KLRG1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rabbit Anti-Human KLRG1 Monoclonal Antibody (Catalog # MAB70293) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human KLRG1 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Reviewed Applications

Read 1 review rated 4 using MAB70293 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KLRG1

KLRG1 (killer cell lectin-like receptor G1), also called MAFA (mast cell function associated), is a 30-38 kDa type II transmembrane inhibitory glycoprotein of the C‑type lectin family, designated CLEC15A. KLRG1 cDNA encodes 195 amino acids (aa) including an intracellular ITIM motif and a 136 aa extracellular domain (ECD) with a single C‑type lectin domain. The human KLRG1 ECD shares 57% and 54% aa identity with mouse and rat KLRG1, respectively. A 189 aa isoform diverges at aa 186. KLRG1 binds E-, N- and R-cadherins and functions as an MHC‑independent means of identifying non-self pathogens and epithelial tumor cells with low E‑cadherin expression. It is expressed as a monomer or disulfide‑linked homodimer on NK and T cell subsets such as tumor-infiltrating lymphocytes.

Long Name

Killer Cell Lectin-like Receptor Subfamily G Member 1

Alternate Names

CLEC15A, MAFA, MAFAL

Entrez Gene IDs

10219 (Human); 50928 (Mouse); 58975 (Rat)

Gene Symbol

KLRG1

UniProt

Q96E93

Additional KLRG1 Products

Product Documents for Human KLRG1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human KLRG1 Antibody

For research use only

Citations for Human KLRG1 Antibody

Customer Reviews for Human KLRG1 Antibody (1)

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  • Human KLRG1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Tonsil
    Species: Human
    Verified Customer | Posted 03/30/2020
    KLRG1 1:50 on human tonsil
    Human KLRG1 Antibody MAB70293

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Protocols

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