Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 697213
View all KLRG1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human KLRG1
Leu60-Phe195
Accession # Q96E93

Specificity

Detects human KLRG1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human KLRG1 Antibody

Detection of Human KLRG1 antibody by Western Blot.

Detection of Human KLRG1 by Western Blot.

Western blot shows lysates of NK-92 human natural killer lymphoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human KLRG1 Monoclonal Antibody (Catalog # MAB70291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for KLRG1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human KLRG1 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: NK‑92 human natural killer lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: KLRG1

KLRG1 (killer cell lectin-like receptor G1), also known as MAFA (mast cell function associated), is a 30-38 kDa type II transmembrane inhibitory glycoprotein of the C‑type lectin family, designated CLEC15A. KLRG1 cDNA encodes 195 amino acids (aa) including an intracellular ITIM motif and a 136 aa extracellular domain (ECD) with a single C-type lectin domain. The human KLRG1 ECD shares 57% and 54% aa identity with mouse and rat KLRG1, respectively. A 189 aa isoform diverges at aa 186. KLRG1 binds E-, N- and R-cadherins and functions as an MHC-independent means of identifying non-self pathogens and epithelial tumor cells with low E-cadherin expression. It is expressed as a monomer or disulfide-linked homodimer on NK and T cell subsets such as tumor-infiltrating lymphocytes.

Long Name

Killer Cell Lectin-like Receptor Subfamily G Member 1

Alternate Names

CLEC15A, MAFA, MAFAL

Entrez Gene IDs

10219 (Human); 50928 (Mouse); 58975 (Rat)

Gene Symbol

KLRG1

UniProt

Q96E93

Additional KLRG1 Products

Product Documents for Human KLRG1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human KLRG1 Antibody

For research use only

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