Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 750835
View all Lgr5/GPR49 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line, CHO derived recombinant human Lgr5/GPR-49
Met1-Ile560
Accession # O75473

Specificity

Detects human Lgr5/GPR-49 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Lgr5/GPR49 MAb (Clone 750835)

Detection of Lgr5/GPR49 antibody in NS0 Mouse Cell Line Transfected with Human Lgr5/GPR49 and eGFP antibody by Flow Cytometry.

Detection of Lgr5/GPR49 in NS0 Mouse Cell Line Transfected with Human Lgr5/GPR49 and eGFP by Flow Cytometry.

NS0 mouse myeloma cell line transfected with (A) human Lgr5/GPR49 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Lgr5/GPR49 Monoclonal Antibody (Catalog # MAB80781) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB0041). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human Lgr5/GPR49 MAb (Clone 750835)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human Lgr5/GPR49 and eGFP

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Lgr5/GPR49

GPR49 (G-protein-coupled receptor 49), also called LGR5 (leucine-rich repeat GPR 5) is a seven-transmembrane glycoprotein receptor that negatively regulates of Wnt signaling in the developing intestine. Expression of GPR49 is upregulated in intestinal stem cells and intestinal cancer stem cells and promotes carcinogenesis. GPR49 cDNA encodes 907 amino acids (aa), including a long N-terminal extracellular domain (aa 22-561) with 16 LRR domains. Human GPR49 shares 90% aa sequence identity with mouse and rat GPR45 within aa 22-561.

Long Name

Leucine-rich Repeat Containing G Protein-coupled Receptor 5

Alternate Names

FEX, GPR49, GPR67, HG38

Entrez Gene IDs

8549 (Human); 14160 (Mouse); 299802 (Rat); 102120216 (Cynomolgus Monkey)

Gene Symbol

LGR5

UniProt

O75473 (Human)

Additional Lgr5/GPR49 Products

Product Documents for Human Lgr5/GPR49 MAb (Clone 750835)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Lgr5/GPR49 MAb (Clone 750835)

For research use only

Customer Reviews for Human Lgr5/GPR49 MAb (Clone 750835)

There are currently no reviews for this product. Be the first to review Human Lgr5/GPR49 MAb (Clone 750835) and earn rewards!

Have you used Human Lgr5/GPR49 MAb (Clone 750835)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies