Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Immunohistochemistry, Neutralization

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all LIF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human LIF
Ser23-Phe202
Accession # P15018

Specificity

Detects human LIF in direct ELISAs.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human LIF Antibody

LIF antibody in Human Lung by Immunohistochemistry (IHC-P).

LIF in Human Lung.

LIF was detected in immersion fixed paraffin-embedded sections of human lung array using Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
LIF antibody in Human Alzheimer's Brain by Immunohistochemistry (IHC-P).

LIF in Human Alzheimer's Brain.

LIF was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody.

Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody.

Recombinant Human LIF (7734-LF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-depend-ent manner (orange line), as measured by Resazurin (AR002). Proliferation elicited by 3 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA). The ND50 is typically 0.04-0.08 µg/mL.
Detection of Human LIF by Western Blot

Detection of Human LIF by Western Blot

LIF promotes Glut1 PM translocation through the activation of AKT signaling.A LIF activated the AKT signaling, which can be blocked by MK2206, an AKT inhibitor (upper panel), or Wortmannin (Wort), a PI3K inhibitor (lower panel), in MCF7, MDA-MB 231 and T47D cells. The levels of p-AKTSer473 (p-AKT), which reflect the activity of AKT were measured by Western-blot assays. B Blocking AKT signaling by MK2206 (left) or Wortmannin (right) largely abolished the promoting effect of LIF on Glut1 PM translocation in breast cancer cells. C, D Blocking AKT signaling by expression of dominant-negative AKT (AKT-DN) largely abolished the promoting effect of LIF on Glut1 PM translocation in breast cancer cells. Cells were transfected with vectors expressing AKT-DN. The levels of p-AKT and total AKT (C), as well as Glut1 PM translocation (D), were determined by Western-blot assays. Uncropped Western-blot images are shown in Supplementary Fig 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35440095), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human LIF by Western Blot

Detection of Human LIF by Western Blot

LIF promotes Glut1 PM translocation through the activation of AKT signaling.A LIF activated the AKT signaling, which can be blocked by MK2206, an AKT inhibitor (upper panel), or Wortmannin (Wort), a PI3K inhibitor (lower panel), in MCF7, MDA-MB 231 and T47D cells. The levels of p-AKTSer473 (p-AKT), which reflect the activity of AKT were measured by Western-blot assays. B Blocking AKT signaling by MK2206 (left) or Wortmannin (right) largely abolished the promoting effect of LIF on Glut1 PM translocation in breast cancer cells. C, D Blocking AKT signaling by expression of dominant-negative AKT (AKT-DN) largely abolished the promoting effect of LIF on Glut1 PM translocation in breast cancer cells. Cells were transfected with vectors expressing AKT-DN. The levels of p-AKT and total AKT (C), as well as Glut1 PM translocation (D), were determined by Western-blot assays. Uncropped Western-blot images are shown in Supplementary Fig 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35440095), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human LIF by Western Blot

Detection of Human LIF by Western Blot

LIF promotes Glut1 PM translocation through the activation of AKT signaling.A LIF activated the AKT signaling, which can be blocked by MK2206, an AKT inhibitor (upper panel), or Wortmannin (Wort), a PI3K inhibitor (lower panel), in MCF7, MDA-MB 231 and T47D cells. The levels of p-AKTSer473 (p-AKT), which reflect the activity of AKT were measured by Western-blot assays. B Blocking AKT signaling by MK2206 (left) or Wortmannin (right) largely abolished the promoting effect of LIF on Glut1 PM translocation in breast cancer cells. C, D Blocking AKT signaling by expression of dominant-negative AKT (AKT-DN) largely abolished the promoting effect of LIF on Glut1 PM translocation in breast cancer cells. Cells were transfected with vectors expressing AKT-DN. The levels of p-AKT and total AKT (C), as well as Glut1 PM translocation (D), were determined by Western-blot assays. Uncropped Western-blot images are shown in Supplementary Fig 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35440095), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human LIF by Western Blot

Detection of Human LIF by Western Blot

LIF promotes Glut1 PM translocation through the activation of AKT signaling.A LIF activated the AKT signaling, which can be blocked by MK2206, an AKT inhibitor (upper panel), or Wortmannin (Wort), a PI3K inhibitor (lower panel), in MCF7, MDA-MB 231 and T47D cells. The levels of p-AKTSer473 (p-AKT), which reflect the activity of AKT were measured by Western-blot assays. B Blocking AKT signaling by MK2206 (left) or Wortmannin (right) largely abolished the promoting effect of LIF on Glut1 PM translocation in breast cancer cells. C, D Blocking AKT signaling by expression of dominant-negative AKT (AKT-DN) largely abolished the promoting effect of LIF on Glut1 PM translocation in breast cancer cells. Cells were transfected with vectors expressing AKT-DN. The levels of p-AKT and total AKT (C), as well as Glut1 PM translocation (D), were determined by Western-blot assays. Uncropped Western-blot images are shown in Supplementary Fig 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35440095), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human LIF Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lung and human Alzheimer's brain subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013).

Neutralization

Measured by its ability to neutralize LIF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.04-0.08 µg/mL in the presence of 3 ng/mL Recombinant Human LIF.

Reviewed Applications

Read 1 review rated 4 using AF-250-NA in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LIF

LIF (Leukemia inhibitory factor; also  Differentiation-stimulating factor) is a 22 kDa (predicted) glycoprotein, member of  the leukemia inhibitory factor/interleukin-6 (LIF/IL-6) family of cytokines. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, it is produced by a variety of cells including T cells, monocytes, fibroblasts, osteoblasts and mast cells. LIF induces hematopoietic differentiation in normal and myeloid leukemia cells, neuronal cell differentiation and stimulation of acute-phase protein synthesis in hepatocytes. Human LIF acts through a receptor comprising a 190 kDa LIF-binding alpha -chain and a 130 kDa signal-transducing beta -chain (gp130), which is shared with the other members of the IL-6 family. Human and mouse LIF exhibit 78% aa sequence identity.

Long Name

Leukemia Inhibitory Factor

Alternate Names

D Factor, Emfilermin, HILDA, MLPLI

Entrez Gene IDs

3976 (Human); 16878 (Mouse); 403449 (Canine)

Gene Symbol

LIF

UniProt

P15018

Additional LIF Products

Product Documents for Human LIF Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human LIF Antibody

For research use only

Citations for Human LIF Antibody

Customer Reviews for Human LIF Antibody (1)

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  • Human LIF Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Skin tissue
    Species: Human
    Verified Customer | Posted 06/19/2021
    Human LIF Antibody AF-250-NA

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