Human LIF Antibody

(9 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human LIF in direct ELISAs. In direct ELISAs, less than 45% cross-reactivity with recombinant mouse LIF and less than
    15% cross-reactivity with recombinant rat LIF is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human LIF
    Ser23-Phe202
    Accession # P15018
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize LIF-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.04-0.08 µg/mL in the presence of 3 ng/mL Recombinant Human LIF.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
LIF in Human Lung. LIF was detected in immersion fixed paraffin-embedded sections of human lung array using Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Immunohistochemistry
LIF in Human Alzheimer's Brain. LIF was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. Recombinant Human LIF (Catalog # 7734-LF) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-depend­ent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by 3 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA). The ND50 is typically
0.04‑0.08 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LIF
LIF (Leukemia inhibitory factor; also  Differentiation-stimulating factor) is a 22 kDa (predicted) glycoprotein, member of  the leukemia inhibitory factor/interleukin-6 (LIF/IL-6) family of cytokines. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, it is produced by a variety of cells including T cells, monocytes, fibroblasts, osteoblasts and mast cells. LIF induces hematopoietic differentiation in normal and myeloid leukemia cells, neuronal cell differentiation and stimulation of acute-phase protein synthesis in hepatocytes. Human LIF acts through a receptor comprising a 190 kDa LIF-binding alpha -chain and a 130 kDa signal-transducing beta -chain (gp130), which is shared with the other members of the IL-6 family. Human and mouse LIF exhibit 78% aa sequence identity.
  • Long Name:
    Leukemia Inhibitory Factor
  • Entrez Gene IDs:
    3976 (Human); 16878 (Mouse); 403449 (Canine)
  • Alternate Names:
    CDF; D factor; DIA; differentiation inhibitory activity; differentiation stimulating factor; Differentiation-stimulating factor; Emfilermin; HILDAcholinergic differentiation factor; leukemia inhibitory factor (cholinergic differentiation factor); leukemia inhibitory factor; LIF; Melanoma-derived LPL inhibitor; MLPLI
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species
Applications
Sample Type
  1. Leukemia inhibitory factor is dysregulated in the endometrium and uterine flushing fluid of patients with adenomyosis during implantation window.
    Authors: Xiao Y, Sun X, Yang X, Zhang J, Xue Q, Cai B, Zhou Y
    Fertil. Steril., 2010;94(1):85-9.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. Effect of levonorgestrel and mifepristone on endometrial receptivity markers in a three-dimensional human endometrial cell culture model.
    Authors: Meng CX, Andersson KL, Bentin-Ley U, Gemzell-Danielsson K, Lalitkumar PG
    Fertil. Steril., 2009;91(1):256-64.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. Disturbances in the LIF pathway in the endometrium among women with unexplained infertility.
    Authors: Aghajanova L, Altmae S, Bjuresten K, Hovatta O, Landgren BM, Stavreus-Evers A
    Fertil. Steril., 2009;91(6):2602-10.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Leukemia inhibitory factor functions as a growth factor in pancreas carcinoma cells: Involvement of regulation of LIF and its receptor expression.
    Authors: Kamohara H, Ogawa M, Ishiko T, Sakamoto K, Baba H
    Int. J. Oncol., 2007;30(4):977-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  5. Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells.
    Authors: Aghajanova L, Skottman H, Stromberg AM, Inzunza J, Lahesmaa R, Hovatta O
    Fertil. Steril., 2006;86(4):1193-209.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
    Authors: He W, Gong K, Smith DK, Ip NY
    FEBS Lett., 2005;579(20):4317-23.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis.
    Authors: Andersson By U, Tani E, Andersson U
    J. Pediatr. Hematol. Oncol., 2004;26(11):706-11.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
    Authors: Sengupta J, Dhawan L, Ghosh D
    Cytokine, 2003;24(6):277-85.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Leukemia inhibitory factor regulates prolactin secretion in prolactinoma and lactotroph cells.
    Authors: Ben-Shlomo A, Miklovsky I, Ren SG, Yong WH, Heaney AP, Culler MD, Melmed S
    J. Clin. Endocrinol. Metab., 2003;88(2):858-63.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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