Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA Capture (Matched Antibody Pair)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 264725
View all LYVE-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LYVE-1
Ser24-Thr238
Accession # Q9Y5Y7

Specificity

Detects human LYVE-1 in ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human LYVE‑1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human LYVE‑1 (Catalog # 2089-LY)
under non-reducing conditions only

Human LYVE-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human LYVE‑1 Biotinylated Antibody (Catalog # BAF2089)
  • Standard: Recombinant Human LYVE-1 Protein, CF (Catalog # 2089-LY)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: LYVE-1

Lymphatic vessel endothelial hyaluronan (HA) receptor-1 (LYVE-1) is a receptor of HA, a linear high molecular weight polymer composed of alternating units of D‑glucuronic acid and N-acetyl-D-glucosamine. HA is found in the extracellular matrix of most animal tissues and in body fluids. It modulates cell behavior and functions during tissue remodeling, development, homeostasis, and disease (1). The turnover of HA (several grams/day in humans) occurs primarily in the lymphatics and liver, the two major clearance systems that catabolize approximately 85% and 15% of HA, respectively (1 - 3). LYVE-1 shares 41% homology with the other known HA receptor, CD44 (4). The homology between the two proteins increases to 61% within the HA binding domain. The HA binding domain, known as the link module, is a common structural motif found in other HA binding proteins such as link protein, aggrecan and versican (1, 5). Human and mouse LYVE-1 share 69% amino acid sequence identity. LYVE-1 is primarily expressed on both the luminal and abluminal surfaces of lymphatic vessels (4, 5). In addition, LYVE-1 is also present in normal hepatic blood sinusoidal endothelial cells (6). LYVE-1 mediates the endocytosis of HA and may transport HA from tissue to lymph by transcytosis, delivering HA to lymphatic capillaries for removal and degradation in the regional lymph nodes (5, 7, 8). Because of its restricted expression patterns, LYVE-1, along with other lymphatic proteins such as VEGF R3, podoplanin and the homeobox protein propero-related (Prox-1), constitute a set of markers useful for distinguishing between lymphatic and blood microvasculature (4, 5, 9 - 11).

References

  1. Knudson, C.B. and W. Knudson (1993) FASEB J. 7:1233.
  2. Evered, D and J. Whelan (1989) Ciba Found. Symp. 143:1.
  3. Laurent, T.C. and J.R.F. Fraser (1992) FASEB J. 6:2397.
  4. Banerji, S. et al. (1999) J. Cell Biol. 144:789.
  5. Prevo, R. et al. (2001) J. Biol. Chem. 276:19420.
  6. Jackson, D.J. et al. (2001)Trends Immunol. 22:317.
  7. Zhou, B. et al. (2000) J. Biol. Chem. 275:37733.
  8. Achen, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95:548.
  9. Breiteneder-Gellef, S. et al. (1999) Am. J. Pathol. 154:385.
  10. Wiggle, J.T. and G. Oliver (1999) Cell 98:769.

Long Name

Lymphatic Vessel Endothelial Hyaluronan Receptor 1

Alternate Names

LYVE1, XLKD1

Entrez Gene IDs

10894 (Human); 114332 (Mouse); 293186 (Rat)

Gene Symbol

LYVE1

UniProt

Q9Y5Y7

Additional LYVE-1 Products

Product Documents for Human LYVE‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human LYVE‑1 Antibody

For research use only

Citations for Human LYVE‑1 Antibody

Customer Reviews for Human LYVE‑1 Antibody

There are currently no reviews for this product. Be the first to review Human LYVE‑1 Antibody and earn rewards!

Have you used Human LYVE‑1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies