Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MED4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MED4
Met80-Asp261
Accession # Q9NPJ6

Specificity

Detects endogenous human MED4 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MED4 Antibody

Detection of Human MED4 antibody by Western Blot.

Detection of Human MED4 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human MED4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5089) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for MED4 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

MED4 antibody in Human Prostate by Immunohistochemistry (IHC-P).

MED4 in Human Prostate.

MED4 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human MED4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5089) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human MED4 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and JEG-3 human epithelial choriocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MED4

MED4 (Mediator complex 4 subunit; also DRIP36) is a 34‑36 kDa member of the mediator complex subunit 4 family of proteins. It is part of a 20+ subunit complex named Mediator that serves as a bridge between RNA polymerase II and DNA binding regulatory proteins that cooperate during RNA synthesis. Human MED4 is 270 amino acids (aa) in length and contains two N-terminal coiled-coil regions (aa 24‑48 and 90‑131) plus a C-terminal poly-Ser region (aa 262‑269). Multiple splice variants may exist. There is an alternate start site at Met47 that may be accompanied by a premature truncation after Asp239. There is also a form with a six aa substitution for aa 105‑270, and a fourth form that shows a 19 aa substitution for aa 1‑41, accompanied by a truncation after Thr260. Over aa 80‑261, human MED4 is 97% aa identical to mouse MED4.

Long Name

Mediator Complex Subunit 4

Alternate Names

ARC36, DRIP36, HSPC126, TRAP36, VDRIP

Entrez Gene IDs

29079 (Human); 67381 (Mouse); 306030 (Rat)

Gene Symbol

MED4

UniProt

Q9NPJ6

Additional MED4 Products

Product Documents for Human MED4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MED4 Antibody

For research use only

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Protocols

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