Human MED4 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human MED4 in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human MED4
    Met80-Asp261
    Accession # Q9NPJ6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human MED4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and JEG‑3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human MED4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5089) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for MED4 at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunohistochemistry
MED4 in Human Prostate. MED4 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human MED4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5089) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MED4

MED4 (Mediator complex 4 subunit; also DRIP36) is a 34‑36 kDa member of the mediator complex subunit 4 family of proteins. It is part of a 20+ subunit complex named Mediator that serves as a bridge between RNA polymerase II and DNA binding regulatory proteins that cooperate during RNA synthesis. Human MED4 is 270 amino acids (aa) in length and contains two N-terminal coiled-coil regions (aa 24‑48 and 90‑131) plus a C-terminal poly-Ser region (aa 262‑269). Multiple splice variants may exist. There is an alternate start site at Met47 that may be accompanied by a premature truncation after Asp239. There is also a form with a six aa substitution for aa 105‑270, and a fourth form that shows a 19 aa substitution for aa 1‑41, accompanied by a truncation after Thr260. Over aa 80‑261, human MED4 is 97% aa identical to mouse MED4.

  • Long Name:
    Mediator Complex Subunit 4
  • Entrez Gene IDs:
    29079 (Human); 67381 (Mouse); 306030 (Rat)
  • Alternate Names:
    Activator-recruited cofactor 36 kDa component; ARC36; DRIP36; DRIP36mediator of RNA polymerase II transcription, subunit 4 homolog (S. cerevisiae); FLJ10956; HSPC126; HSPC126vitamin D receptor interacting protein; MED4; mediator complex subunit 4TRAP/SMCC/PC2 subunit p36 subunit; subunit 4 homolog; TRAP36; VDRIP
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