Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1059503
View all mGluR1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide
Accession # P42261

Specificity

Detects mouse GluR1 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human mGluR1 Antibody

Detection of mGluR1 in Human Hippocampus.

mGluR1 was detected in immersion fixed paraffin-embedded sections of Human Hippocampus using Mouse Anti-Human mGluR1 Monoclonal Antibody (Catalog # MAB48362) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm in neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human mGluR1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of Human Hippocampus

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: mGluR1

GluR1 is a 4-transmembrane protein that belongs to the glutamate-gated ion channel family. It is one of four AMPA receptor subunits that form a functional heterotetrameric glutamate receptor. GluR1 only interacts with GluR2. In addition to constitutive phosphorylation on a key serine in the C-terminal extracellular region, neurotransmitter-induced phosphorylation of another key serine by CaMKII leads to a potentiation of glutamate-mediated current.

Long Name

Metabotropic Glutamate Receptor 1

Alternate Names

GPRC1A, GRM1

Entrez Gene IDs

2911 (Human); 14816 (Mouse); 24414 (Rat)

Gene Symbol

GRM1

UniProt

P42261 (Human)

Additional mGluR1 Products

Product Documents for Human mGluR1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human mGluR1 Antibody

For research use only

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Protocols

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