Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 437310
View all mGluR7 Primary Antibodies »

Product Specifications

Immunogen

HEK293 human embryonic kidney cell line transfected with human mGluR7
Gln35-Ile915
Accession # Q14831

Specificity

Detects human mGluR7. Stains human mGluR7 transfectants but not irrelevant transfectants.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human mGluR7 Antibody

Detection of mGluR7 antibody in A172 Human Cell Line antibody by Flow Cytometry.

Detection of mGluR7 in A172 Human Cell Line by Flow Cytometry.

A172 human glioblastoma cell line was stained with Human mGluR7 Monoclonal Antibody (Catalog # MAB4405, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Applications for Human mGluR7 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: A172 human glioblastoma cell line

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: mGluR7

Metabotropic glutamate receptor 7 (mGluR7), also called GRM7 (G protein-coupled metabotropic glutamate receptor 7) is a 102 kDa member of the GRM family of 7‑transmembrane G protein-coupled receptors. mGluR7 is concentrated in the hippocampus and the amygdala and has a putative role in anxiety and spatial working memory. Within the 555 aa N-terminal extracellular domain, human mGluR7 shares > 99% aa identity with mouse, rat and dog mGluR7 and 90% aa identity with chicken mGluR7. Five isoforms (911‑924 aa) vary at the C‑terminus and are differentially expressed.

Long Name

Metabotropic Glutamate Receptor 7

Alternate Names

GPRC1G, GRM7

Entrez Gene IDs

2917 (Human); 108073 (Mouse); 81672 (Rat)

Gene Symbol

GRM7

UniProt

Q14831

Additional mGluR7 Products

Product Documents for Human mGluR7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human mGluR7 Antibody

For research use only

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