Human MIA Antibody

Catalog # Availability Size / Price Qty
MAB20501
MAB20501-SP
Human MIA Antibody in Immunohistochemistry
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Product Details
Citations (2)
FAQs
Supplemental Products
Reviews (1)

Human MIA Antibody Summary

Species Reactivity
Human
Specificity
Detects human MIA in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL-5 or recombinant rat IL-5 is observed.
Source
Monoclonal Mouse IgG2B Clone # 294203
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MIA
Gly25-Gln131
Accession # Q16674
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry MIA in Human Pancreas. View Larger

MIA in Human Pancreas. MIA was detected in immersion fixed paraffin-embedded sections of human pancreas array using Mouse Anti-Human MIA Monoclonal Antibody (Catalog # MAB20501) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIA

MIA, also named Cartilage-Derived Retinoic Acid-Sensitive Protein (CD-RAP), is a secreted protein that plays an important role in melanoma metastasis. It is highly expressed in malignant melanomas and is associated with tumor progression.

Long Name
Melanoma Inhibitory Activity
Entrez Gene IDs
8190 (Human); 12587 (Mouse)
Alternate Names
CD-RAP; Melanoma inhibitory activity protein; melanoma inhibitory activity; melanoma-derived growth regulatory protein; MIA

Product Datasheets

Citations for Human MIA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Storkhead box 2 and melanoma inhibitory activity promote oral squamous cell carcinoma progression
    Oncotarget, 2016;7(18):26751-64.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. MIA is a potential biomarker for tumour load in neurofibromatosis type 1.
    Authors: Kolanczyk M, Mautner V, Kossler N, Nguyen R, Kuhnisch J, Zemojtel T, Jamsheer A, Wegener E, Thurisch B, Tinschert S, Holtkamp N, Park SJ, Birch P, Kendler D, Harder A, Mundlos S, Kluwe L
    BMC Med, 2011;9(0):82.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human MIA Antibody

Average Rating: 4 (Based on 1 Review)

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Human MIA Antibody
By Anonymous on 05/22/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human

AF2050 was tested along with MAB20501 in a sandwich assay. Although both antibodies worked as either the capture or detection, AF2050 had significantly higher affinity, which allowed quantification at a lower dilution of samples. The version of the assay we ended up using was AF2050 as both the capture and detection.