Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MOG Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human MOG
Gly30-Gly154 (Asp131Ala)
Accession # Q8IYG5

Specificity

Detects human MOG in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human MOG Antibody

MOG in Human Brain (Cerebellum).

MOG was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Goat Anti-Human MOG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2395) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human MOG Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (cerebellum)

Western Blot

0.1 µg/mL
Sample: Recombinant Human MOG

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MOG

MOG is an Ig superfamily member found exclusively in myelinating oligodendrocytes in the central nervous system. Multiple isoforms of human MOG exist. The amino-terminal extracellular domain of human MOG shares 92% and 91% amino acid sequence homology with the corresponding regions of the mouse and rat protein, respectively.

Long Name

Myelin Oligodendrocyte Glycoprotein

Alternate Names

MGC26137, MOG Ig-AluB, MOGIG2, myelin oligodendrocyte glycoprotein, myelin-oligodendrocyte glycoprotein

Entrez Gene IDs

4340 (Human); 17441 (Mouse)

Gene Symbol

MOG

UniProt

Q8IYG5

Additional MOG Products

Product Documents for Human MOG Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human MOG Antibody

For research use only

Citations for Human MOG Antibody

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Protocols

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