Key Product Details

Species Reactivity

Human, Mouse

Applications

Multiplex Immunofluorescence, Immunohistochemistry, Western Blot, COMET

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1006132
View all Cadherin Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human E‑Cadherin
Accession # P12830

Specificity

Detects human E-Cadherin, P-Cadherin, N-Cadherin and mouse E-Cadherin in Western blots. Due to the high homology of the immunogen used, this antibody is designed to be pan specific to Cadherins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse Cadherin Pan Specific Antibody

Detection of Cadherin in Human Liver via Multiplex Immunofluorescence staining on COMET™

Cadherin was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human/Mouse Cadherin Pan Specific Monoclonal Antibody (MAB18385) at 15 μg/mL at 37° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Cadherin in Human Colon via Multiplex Immunofluorescence staining on COMET™

Cadherin was detected in immersion fixed paraffin-embedded sections of human colon using Mouse Anti-Human/Mouse Cadherin Pan Specific Monoclonal Antibody (MAB18385) at 15 μg/mL at 37° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Cadherin in Human Colon Cancer via Multiplex Immunofluorescence staining on COMET™

Cadherin was detected in immersion fixed paraffin-embedded sections of human colon cancer using Mouse Anti-Human/Mouse Cadherin Pan Specific Monoclonal Antibody (MAB18385) at 15 μg/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.

Detection of Cadherin in Mouse Stomach via seqIF™ staining on COMET™​

Cadherin Antibody was detected in immersion fixed paraffin-embedded sections of Mouse Stomach using Mouse Anti-Human/Mouse E-Cadherin, Monoclonal Antibody (Catalog #MAB18385) at 15ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder.​​
Detection of Human and Mouse Cadherin antibody by Western Blot.

Detection of Human and Mouse Cadherin by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MCF-7 human breast cancer cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Cadherin Pan Specific Monoclonal Antibody (Catalog # MAB18385) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). Specific bands were detected for Cadherin at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cadherin antibody in Human Cystadenocarcinoma by Immunohistochemistry (IHC-P).

Cadherin in Human Cystadenocarcinoma.

Cadherin was detected in immersion fixed paraffin-embedded sections of human papillary serous cystadenocarcinoma of the colon using Mouse Anti-Human/Mouse Cadherin Pan Specific Monoclonal Antibody (Catalog # MAB18385) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Human Cadherins antibody by Western Blot.

Detection of Human Cadherins by Western Blot.

Western blot shows lysates of HEK293T human embryonic kidney cell line mock transfected or transfected with human E-Cadherin and NS0 mouse myeloma cell line transfected with Human P-Cadherin, N-Cadherin, or mock transfected. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse Cadherin Pan Specific Monoclonal Antibody (Catalog # MAB18385) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Cadherin at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse Cadherin Pan Specific Antibody

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed human papillary serous cystadenocarcinoma of the colon

Multiplex Immunofluorescence

15 µg/mL
Sample: Immersion fixed paraffin embedded sections of human liver, human colon, human colon cancer, and mouse stomach

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, MCF‑7 human breast cancer cell line, C2C12 mouse myoblast cell line, HEK293T human embryonic kidney cell line, HEK293T human embryonic kidney cell line transfected with human E-Cadherin, and NS0 mouse myeloma cell line transfected with Human P-Cadherin or N-Cadherin

Reviewed Applications

Read 1 review rated 5 using MAB18385 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Cadherin

Epithelial (E)‑Cadherin (ECAD), also known as Cadherin-1, cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules (gene name CDH1). Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each (amino acids 155-697).

References

  1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
  2. Overduin, M. et al. (1995) Science 267:386.
  3. Takeichi, M. (1991) Science 251:1451.

Long Name

Cadherin Pan Specific

Alternate Names

Arc-1, cadherin 1, E-cadherin (epithelial), cadherin 1, type 1, E-cadherin (epithelial), cadherin-1, calcium-dependent adhesion protein, epithelial, CAM 120/80, CD324, CD324 antigen, CDHE, cell-CAM 120/80, ECAD, E-cadherin, Epithelial cadherin, LCAM, UVOE-Cadherin, uvomorulin

Entrez Gene IDs

999 (Human)

Gene Symbol

CDH1

UniProt

P12830

Additional Cadherin Products

Product Documents for Human/Mouse Cadherin Pan Specific Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse Cadherin Pan Specific Antibody

For research use only

Related Research Areas

Adhesion Molecules
Cadherin Superfamily

Customer Reviews for Human/Mouse Cadherin Pan Specific Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human/Mouse Cadherin Pan Specific Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Human/Mouse Cadherin Pan Specific Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Colon tissue
    Species: Mouse
    Verified Customer | Posted 12/24/2021
    Human/Mouse Cadherin Pan Specific Antibody MAB18385

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies