Human/Mouse COX-2 Antibody

Catalog # Availability Size / Price Qty
AF4198
AF4198-SP
Human/Mouse COX-2 Antibody in Data
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Product Details
Citations (5)
FAQs
Supplemental Products
Reviews (3)

Human/Mouse COX-2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse COX-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human COX-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human COX-2
Ala18-Leu604
Accession # P35354
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
5-15 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human and Mouse COX‑2 by Western Blot. View Larger

Detection of Human and Mouse COX‑2 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cell (PBMC) and RAW 264.7 mouse monocyte/macrophage cell line untreated (-) or treated (+) with 1 ug/mL LPS for 24 hours and U937 human histiocytic lymphoma cell line untreated or treated with 100 nM PMA and 1 ug/mL LPS for 48 hours and 24 hours, respectively. PVDF membrane was probed with 1 µg/mL of Human/Mouse COX-2 Polyclonal Antibody (Catalog # AF4198), followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for COX-2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry COX‑2 in HUVEC Human Cells. View Larger

COX‑2 in HUVEC Human Cells. COX-2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using 10 µg/mL Human/Mouse COX-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4198) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry COX‑2 in RAW 264.7 Mouse Cells. View Larger

COX‑2 in RAW 264.7 Mouse Cells. COX-2 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cells stimulated with LPS using Goat Anti-Human/Mouse COX-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4198) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Intracellular Staining by Flow Cytometry Detection of COX‑2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. View Larger

Detection of COX‑2 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line treated with 1 µg/mL LPS for 24 hours was stained with Goat Anti-Human/Mouse COX-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4198, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: COX-2

Cyclooxygenase-2 (COX-2) also known as prostaglandin G/H synthase 2 (PGHS2) is a 70 kDa microsomal enzyme that belongs to the prostaglandin G/H synthase family. It is inducibly-expressed by a number of cell types, including fibroblasts, vascular smooth muscle cells, endothelium, and monocytes. Functionally, COX-2 is a homodimer that catalyzes two steps in the conversion of arachadonic acid to prostaglandin H2. Mature human COX-2 is 587 amino acids (aa) in length and contains one EGF-like domain (aa 18‑55), a potential membrane interacting region (aa 277‑292) and a globular catalytic domain (aa 293‑604). At least one splice form exists that shows an 11 aa substitution for the C-terminal 451 amino acids. Mature human COX-2 shows 87% aa identity to mouse COX-2.

Long Name
Cyclooxygenase 2
Entrez Gene IDs
5743 (Human); 19225 (Mouse); 29527 (Rat)
Alternate Names
COX2; COX-2; COX2cyclooxygenase 2b; cyclooxygenase-2; EC 1.14.99; EC 1.14.99.1; GRIPGHS; hCox-2; PGG/HS; PGH synthase 2; PGHS-2; PHS II; PHS-2; PHS-II; prostaglandin G/H synthase 2; prostaglandin G/H synthase and cyclooxygenase; Prostaglandin H2 synthase 2; prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase andcyclooxygenase); Prostaglandin-endoperoxide synthase 2; PTGS2

Product Datasheets

Citations for Human/Mouse COX-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Multi-Staged Regulation of Lipid Signaling Mediators during Myogenesis by COX-1/2 Pathways
    Authors: C Mo, Z Wang, L Bonewald, M Brotto
    Int J Mol Sci, 2019;20(18):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Increased Toxoplasma gondii Intracellular Proliferation in Human Extravillous Trophoblast Cells (HTR8/SVneo Line) Is Sequentially Triggered by MIF, ERK1/2, and COX-2
    Authors: ICB Milian, RJ Silva, C Manzan-Mar, BF Barbosa, PM Guirelli, M Ribeiro, A de Oliveir, F Ietta, JR Mineo, P Silva Fran, EAV Ferro
    Front Microbiol, 2019;10(0):852.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. Response gene to complement 32 expression in macrophages augments paracrine stimulation-mediated colon cancer progression
    Authors: P Zhao, B Wang, Z Zhang, W Zhang, Y Liu
    Cell Death Dis, 2019;10(10):776.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. TET2 Regulates the Neuroinflammatory Response in Microglia
    Authors: A Carrillo-J, Ö Deniz, MV Niklison-C, R Ruiz, K Bezerra-Sa, V Stratoulia, R Amouroux, PK Yip, A Vilalta, M Cheray, AM Scott-Eger, E Rivas, K Tayara, I García-Dom, J Garcia-Rev, JC Fernandez-, AM Espinosa-O, X Shen, P St George-, GC Brown, P Hajkova, B Joseph, JL Venero, MR Branco, MA Burguillos
    Cell Rep, 2019;29(3):697-713.e8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Stromal versus tumoral inflammation differentially contribute to metastasis and poor survival in laryngeal squamous cell carcinoma
    Authors: B Höing, O Kanaan, P Altenhoff, R Petri, K Thangavelu, A Schlüter, S Lang, A Bankfalvi, S Brandau
    Oncotarget, 2018;9(9):8415-8426.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Reviews for Human/Mouse COX-2 Antibody

Average Rating: 4.3 (Based on 3 Reviews)

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Human/Mouse COX-2 Antibody
By Anonymous on 11/04/2020
Application: ICC/IF Sample Tested: Melanoma tissue Species: Human

Human/Mouse COX-2 Antibody
By Anonymous on 07/30/2019
Application: WB Sample Tested: Adipose tissue Species: Mouse

Human/Mouse COX-2 Antibody
By Anonymous on 05/15/2017
Application: ICC/IF Sample Tested: Spleen tissue Species: Mouse