Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all COX4-I1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human COX4-I1
Ala23-Lys169
Accession # P13073

Specificity

Detects human and mouse COX-4I1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse COX4‑I1 Antibody

Detection of Human/Mouse COX4-I1 antibody by Western Blot.

Detection of Human/Mouse COX4-I1 by Western Blot.

Western blot shows lysates of human brain tissue, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse COX4-I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5814) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for COX4-I1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
COX4-I1 antibody in Human Kidney by Immunohistochemistry (IHC-P).

COX4-I1 in Human Kidney.

COX4-I1 was detected in immersion fixed paraffin-embedded sections of normal human kidney using Goat Anti-Human/Mouse COX4-I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5814) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
COX4-I1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

COX4‑I1 in HeLa Human Cell Line.

COX4-I1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse COX4-I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5814) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to mitochondria. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human COX4‑I1 antibody by Simple WesternTM.

Detection of Human COX4‑I1 by Simple WesternTM.

Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for COX4-I1 at approximately 24 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse COX4-I1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5814) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse COX4‑I1 Antibody

Application
Recommended Usage

Immunocytochemistry

This antibody has been used at a concentration of 5-15 μg/mL to detect COX4-I1 in immersion fixed HeLa human cervical epithelial carcinoma cell line. Internal testing was not able to validate staining in a mouse cell line.

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of normal human kidney

Simple Western

10 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: Human brain tissue, Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, and BaF3 mouse pro-B cell line

Reviewed Applications

Read 2 reviews rated 4 using AF5814 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: COX4-I1

Cytochrome c oxidase subunit 4 isoform 1 (COX-4I1) is a 21‑22 kDa member of the cytochrome c oxidase IV family of proteins. It is a component of COX, an inner mitochondrial membrane multimeric dimer that catalyzes the transfer of electrons from cytochrome c to dioxygen. COX-4I1 is the largest of 10 distinct nuclear DNA‑encoded subunits that form each COX monomer. Human COX4-I1 is 169 amino acids (aa) in length and possesses a mitochondrial transit peptide between aa 1‑22, an ATP binding site (aa 42; 95‑100), and multiple subunit interface sequences. The ATP binding site is suggested to make COX-4I1 a regulatory subunit within the COX complex. There are two potential splice variants for COX-4I1 that show a three aa substitution for aa 81‑169, and a single Gly substitution for aa 125‑169. COX‑4I2, the product of a different gene, shares only 50% aa identity with COX-4I1. Over amino acid 23‑169, human COX4‑1 shares 78% aa identity with mouse COX‑4I1.

Long Name

Cytochrome c Oxidase Subunit IV Isoform 1

Alternate Names

COX IV-1, COX4I1

Entrez Gene IDs

1327 (Human); 12857 (Mouse); 29445 (Rat)

Gene Symbol

COX4I1

UniProt

P13073 (Human)

Additional COX4-I1 Products

Product Documents for Human/Mouse COX4‑I1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse COX4‑I1 Antibody

For research use only

Citations for Human/Mouse COX4‑I1 Antibody

Customer Reviews for Human/Mouse COX4‑I1 Antibody (2)

4 out of 5
2 Customer Ratings
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Customer Images

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  • Human/Mouse COX4-I1 Antibody
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: NSC-34 cells
    Species: Human and Mouse
    Verified Customer | Posted 09/18/2018
    NSC-34 cells were cultured and fixed in methanol before immunofluorescence staining was conducted. COXIV antibody was used at 1 in 50 and no primary antibody and secondary antibody controls were conducted. Imaging was done on a confocal microscope.
    PBS 0.01% Triton 0.3% Fish Skin Gelatin
    Human/Mouse COX4‑I1 Antibody AF5814
  • Human/Mouse COX4-I1 Antibody
    Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: NSC-34 cells
    Species: Human and Mouse
    Verified Customer | Posted 09/18/2018
    NSC-34 cells were cultured and fixed in methanol before immunofluorescence staining was conducted. COXIV antibody was used at 1 in 50 and no primary antibody and secondary antibody controls were conducted. Imaging was done on a confocal microscope.
    Human/Mouse COX4‑I1 Antibody AF5814

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Protocols

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